Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-19T10:01:15.541Z Has data issue: false hasContentIssue false
  • English
  • Français

Streptococcus spp. from bulk-tank milk and milking machine teatcups on small ruminant farms, and factors potentially associated with their isolation

Published online by Cambridge University Press:  04 September 2020

Dimitris C. Chatzopoulos ,
Daphne T. Lianou ,
Charalambia K. Michael ,
Dimitris A. Gougoulis ,
Vasia S. Mavrogianni ,
Natalia G. C. Vasileiou ,
Angeliki I. Katsafadou ,
Antonis P. Politis ,
Nikos G. Kordalis  and
Eleni I. Katsarou
...Show all authors
Dimitris C. Chatzopoulos
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Daphne T. Lianou
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Charalambia K. Michael
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Dimitris A. Gougoulis
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Vasia S. Mavrogianni
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Natalia G. C. Vasileiou
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Angeliki I. Katsafadou
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Antonis P. Politis
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Nikos G. Kordalis
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Eleni I. Katsarou
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Katerina S. Ioannidi
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Efthimia Petinaki
Affiliation:
University Hospital of Larissa, Larissa, Greece
George C. Fthenakis*
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
*
Author for correspondence: George C. Fthenakis, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The objectives of this work were (a) to determine the presence of streptococci in samples from small ruminant dairy farms (bulk-tank milk and, where possible, teatcup swabs), (b) to investigate the potential adverse effects of streptococci on milk quality and (c) to investigate the importance of some husbandry factors for the isolation of streptococci. Bulk-tank milk samples and teatcups swab samples were examined bacteriologically for the presence of streptococci. Somatic cell counting and milk composition measurements were also performed. The husbandry factors present in each farm were assessed for potential associations with the isolation of streptococci. Streptococci were isolated from milk samples from 31.4% of sheep and 17.4% of goat farms and from 4.8% of sheep and 5.9% of goat teatcups. Streptococci were isolated more frequently from the upper part than the lower part of teatcups: 5.0% vs. 1.9%. Most isolates (57.9%) were identified as Streptococcus uberis. Most isolates (68.4%) were slime-producing; slime-production was more frequent among isolates from teatcups (83.3%) than from bulk-tank milk (55.0%). Somatic cell counts and milk composition did not differ between farms in which streptococci were or were not isolated. Machine-milking was associated with the isolation of streptococci from bulk-tank milk samples. The initial stage of the milking period (first two months) was found to be associated with the isolation of streptococci from milking machine teatcups in sheep farms only.

Keywords

Goatintramammary infectionmastitissheepsomatic cell countsStreptococcussubclinical mastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 3 , August 2020 , pp. 277 - 281
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

These authors have contributed equally and their names are listed alphabetically

References

Addis, MF, Pisanu, S, Marogna, G, Cubeddu, T, Pagnozzi, D, Cacciotto, C, Campesi, F, Schianchi, G, Rocca, S and Uzzau, S (2013) Production and release of antimicrobial and immune defense proteins by mammary epithelial cells following Streptococcus uberis infection of sheep. Infection and Immunity 81, 3182–3197.CrossRefGoogle ScholarPubMed
Albenzio, M, Santillo, A, Caroprese, M, Ciliberti, MG, Marino, R and Sevi, A (2016) Effect of stage of lactation on the immune competence of goat mammary gland. Journal of Dairy Science 99, 3889–3895.CrossRefGoogle ScholarPubMed
Barrow, GI and Feltham, RKA (1993) Manual for the Identification of Medical Bacteria, 3rd Edn. Cambridge, UK: Cambridge University Press.Google Scholar
Bramley, AJ (1982) Sources of Streptococcus uberis in the dairy herd: I. Isolation from bovine faeces and from straw bedding of cattle. Journal of Dairy Research 49, 369–373.CrossRefGoogle ScholarPubMed
Devriese, LA, Vandamme, P, Collins, MD, Alvarez, N, Pot, B, Hommez, J, Butaye, P and Haesebrouck, F (1999) Streptococcus pluranimalium sp. nov., from cattle and other animals. International Journal of Systematic Bacteriology 49, 1221–1226.Google ScholarPubMed
Dieser, SA, Fessia, AS, Zanotti, AR, Raspanti, CG and Odierno, LM (2019) Fibronectin and laminin induce biofilm formation by Streptococcus uberis and decrease its penicillin susceptibility. Microbial Pathogenesis 136, 103652.CrossRefGoogle ScholarPubMed
Euzeby, JP (1997) List of bacterial names with standing in nomenclature: a folder available on the Internet. International Journal of Systematic Bacteriology 47, 590–592. (list of prokaryotic names with standing in nomenclature, http://www.bacterio.net).Google ScholarPubMed
Fernandez, E, Blume, V, Garrido, P, Collins, MD, Mateos, A, Dominguez, L and Fernandez-Garayzabal, JF (2004) Streptococcus equi subsp. ruminatorum Subsp. nov., isolated from mastitis in small ruminants. International Journal of Systematic and Evolutionary Microbiology 54, 2291–2296.CrossRefGoogle ScholarPubMed
Freeman, DJ, Falkiner, FR and Keane, CT (1989) New method of detecting slime production by coagulase negative staphylococci. Journal of Clinical Pathology 42, 872–874.CrossRefGoogle ScholarPubMed
Gelasakis, AI, Mavrogianni, VS, Petridis, IG, Vasileiou, NGC and Fthenakis, GC (2015) Mastitis in sheep – The last 10 years and the future of research. Veterinary Microbiology 185, 136–146.CrossRefGoogle Scholar
Hickey, EE, Wong, HS, Khazandi, M, Ogunniyi, AD, Petrovski, KR, Garg, S, Page, SW, O'Handley, R and Trott, DJ (2018) Repurposing ionophores as novel antimicrobial agents for the treatment of bovine mastitis caused by Gram-positive pathogens. Journal of Veterinary Pharmacology and Therapeutics 41, 746–754.Google ScholarPubMed
Hillerton, JE (2020) Timing of entry of Streptococcus uberis into the mammary gland of the dairy cow. Journal of Dairy Research 87. doi: 10.1017/S0022029920000357.Google Scholar
Kaczorek, E, Małaczewska, J, Wójcik, R and Siwicki, AK (2017) Biofilm production and other virulence factors in Streptococcus Spp. isolated from clinical cases of bovine mastitis in Poland. BMC Veterinary Research 13, 398.Google ScholarPubMed
Kruze, J and Bramley, AJ (1982) Sources of Streptococcus uberis in the dairy herd: I. Evidence of colonization of the bovine intestine by Str. uberis. Journal of Dairy Research 49, 375–379.CrossRefGoogle ScholarPubMed
Marogna, G, Pilo, C, Vidili, A, Tola, S, Schianchi, G and Leori, SG (2012) Comparison of clinical findings, microbiological results, and farming parameters in goat herds affected by recurrent infectious mastitis. Small Ruminant Research 102, 74–83.CrossRefGoogle Scholar
Pisanu, S, Cubeddu, T, Pagnozzi, D, Rocca, S, Cacciotto, C, Alberti, A, Marogna, G, Uzzau, S and Addis, MF (2015) Neutrophil extracellular traps in sheep mastitis. Veterinary Research 46, 59.CrossRefGoogle ScholarPubMed
Queiroga, MC (2017) Prevalence and aetiology of sheep mastitis in Alentejo region of Portugal. Small Ruminant Research 153, 123–130.CrossRefGoogle Scholar
Santos, Al, Pires, ACS, Behaine, JJS, Araújo, EA, de Andrade, NJ and de Carvalho, AF (2013) Effect of cleaning treatment on adhesion of Streptococcus agalactiae to milking machine surfaces. Food and Bioprocess Technology 6, 1868–1872.CrossRefGoogle Scholar
Schönborn, S, Wente, N, Paduch, JH and Krömker, V (2017) In vitro ability of mastitis causing pathogens to form biofilms. Journal of Dairy Research 84, 198–201.CrossRefGoogle ScholarPubMed
Vasileiou, NGC, Cripps, PJ, Ioannidi, KS, Chatzopoulos, DC, Gougoulis, DA, Sarrou, S, Orfanou, DC, Politis, A, Calvo Gonzalez-Valerio, T, Argyros, S, Mavrogianni, VS, Petinaki, E and Fthenakis, GC (2018) Extensive countrywide field investigation of subclinical mastitis in sheep in Greece. Journal of Dairy Science 101, 7297–7310.CrossRefGoogle Scholar
Vasileiou, NGC, Chatzopoulos, DC, Sarrou, S, Fragkou, IA, Katsafadou, AI, Mavrogianni, VS, Petinaki, E and Fthenakis, GC (2019) Role of staphylococci in mastitis in sheep. Journal of Dairy Research 86, 254–266.CrossRefGoogle Scholar
Supplementary material: PDF

Chatzopoulos et al. supplementary material

Chatzopoulos et al. supplementary material

Download Chatzopoulos et al. supplementary material(PDF)
PDF 121.5 KB