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Separation of the submicelles from micellar casein by high performance gel chromatography on a TSK-GEL G4000SW column

Published online by Cambridge University Press:  01 June 2009

Tomotada Ono ,
Satoshi Odagiri  and
Toshio Takagi
Tomotada Ono
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate 020, Japan
Satoshi Odagiri
Affiliation:
Department of Agricultural Chemistry, Iwate University, Morioka, Iwate 020, Japan
Toshio Takagi
Affiliation:
Institute for Protein Research, Osaka University, Suita, Osaka 565, Japan
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Summary

Bovine caseins from skim-milk (micellar casein and acid precipitated casein) were separated into 4 discrete components by high performance gel chromatography on a TSK-GEL G4000SW columm. The resolution was better than that obtained by conventional gel chromatography which resulted in separation into 2 components at best. Our results showed that the second and third components consisted of αs1- and κ-caseins and αs1- and β-caseins respectively. They are presumed to be submicellar fractions. Samples prepared by removal of Ca phosphate from large and small micelles were rich in the third and the second components respectively. It is suggested that casein micelles consist of αs1- and κ-casein complexes and complexes and polymers of αs1- and β-casein which are situated preferentially on the periphery and in the interior of the micelles respectively.

Type
Original Articles
Information
Journal of Dairy Research , Volume 50 , Issue 1 , February 1983 , pp. 37 - 44
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

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References

REFERENCES

Aschaffenburg, R. 1963 Preparation of β-casein by a modified urea fractionation method. Journal of Dairy Research 30 259–260CrossRefGoogle Scholar
Chiba, H., Tatsumi, K., Sasaki, R. & Sugimoto, E. 1970 Chemical modification αs- and κ-caseins. Journal of the Agricultural Chemical Society of Japan 44 371–379 (Dairy Science Abstracts 34 772)Google Scholar
Creamer, L. K. & Berry, G. P. 1975 A study of the properties of dissociated bovine casein micelles. Journal of Dairy Research 42 169–183CrossRefGoogle Scholar
Downey, W. K. & Murphy, R. F. 1970 Association of lipases with micellar and soluble casein complexes. Journal of Dairy Research 37 47–59CrossRefGoogle Scholar
Fox, P. F. & Guiney, J. 1972 A procedure for the partial fractionation of the αs-casein complex. Journal of Dairy Research 39 49–53CrossRefGoogle Scholar
Fukano, K., Komiya, K., Sasaki, H. & Hashimoto, T. 1978 Evaluation of new supports for high-pressure aqueous gel permeation chromatography: TSK-GEL SW type columns. Journal of Chromatography 166 47–54CrossRefGoogle Scholar
Gordon, A. H. 1969 Electrophoresis of proteins in polyaerylamide and starch gels, pp. 34–64. (Laboratory Techniques in Biochemistry and Molecular Biology Vol. 1, part 1) Amsterdam: North-Holland Publishing Co.Google Scholar
Jenness, R. & Koops, J. 1962 Preparation and properties of a salt solution which simulates milk ultrafiltrate. Netherlands Milk and Dairy Journal 16 153–164Google Scholar
Nakai, S. & Van De Voort, F. 1979 Application of multiple regression analysis to sedimentation equilibrium data of αs1- and κ-casein interactions for calculation of molecular weight distributions. Journal of Dairy Research 46 283–290CrossRefGoogle Scholar
Ono, T., Dan, H. T. & Odagiri, S. 1978 Dissociation of bovine casein micelles by dialysis. Agricultural & Biological Chemistry 42 1063–1064Google Scholar
Ono, T., Yutani, K. & Odagiri, S. 1974 Conformation of αs-casein in various concentrations. Agricultural & Biological Chemistry 38 1609–1616Google Scholar
Schmidt, D. G. 1969 Effect of the addition of αs1-,β- and κ-casein on the size of the casein micelles in UHTST-sterilized concentrated skim milk. Netherlands Milk and Dairy Journal 23 128–135Google Scholar
Schmidt, D. G. 1980 Colloidal aspects of casein. Netherlands Milk and Dairy Journal 34 42–64Google Scholar
Schmidt, D. G. & Buchheim, W. 1970 [An electron-microscopical investigation of the sub-structure of the casein micelles in cow's milk.] Milchmissenschaft 25 596–600Google Scholar
Schmidt, D. G., Koops, J. & Westbrbeek, D. 1977 Properties of artificial casein micelles. 1. Preparation, size distribution and composition. Netherlands Milk and Dairy Journal 31 328–341Google Scholar
Schultz, B. C. & Bloomfield, V. A. 1976 Structure of bovine casein oligomers. Archives of Biochemistry and Biophysics 173 18–26CrossRefGoogle ScholarPubMed
Shimmin, P. D. & Hill, R. D. 1964 An electron microscope study of the internal structure of casein micelles. Journal of Dairy Research 31 121–123CrossRefGoogle Scholar
Slattery, C. W. & Evard, R. 1973 A model for the formation and structure of casein micelles from subunits of variable composition. Biochimica et Biophysica Acta 317 529–538CrossRefGoogle Scholar
Sullivan, R. A., Fitzpatrick, M. M. & Stanton, E. K. 1959 Distribution of kappa-casein in skim milk. Nature 183 616–617CrossRefGoogle ScholarPubMed
Takagi, T. 1981 Confirmation of molecular weight of Aspergillus oryzae α-amylase using the low angle laser light scattering technique in combination with high pressure silica gel chromatography. Journal of Biochemistry 89 363–368CrossRefGoogle ScholarPubMed
Takase, K., Niki, R. & Arima, S. 1980 A sedimentation equilibrium study of the temperature-dependent association of bovine β-casein. Biochimica et Biophysica Acta 622 1–8CrossRefGoogle ScholarPubMed
Waugh, D. F., Creamer, L. K., Slattery, C. W. & Dresdner, G. W. 1970 Core polymers of casein micelles. Biochemistry 9 786–795CrossRefGoogle ScholarPubMed
Waugh, D. F. & Talbot, B. 1971 Equilibrium casein micelle systems. Biochemistry 10 4153–4162CrossRefGoogle ScholarPubMed
Zittle, C. A. & Custer, J. H. 1963 Purification and some of the properties of αs-casein and κ-casein. Journal of Dairy Science 46 1183–1188CrossRefGoogle Scholar