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Sandwich enzyme immunoassay of Mucor miehei proteinase (Fromase) in cheese

Published online by Cambridge University Press:  01 June 2009

Pavel Rauch ,
Igor Hochel ,
Eva Beránková  and
Jan Káš
Pavel Rauch
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Igor Hochel
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Eva Beránková
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Jan Káš
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
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Summary

Sandwich enzyme immunoassay of Mucor miehei proteinase (from the commercial preparation Fromase) has been developed using horseradish peroxidase as a marker. The enzyme may be determined to a detection limit of 50 ng/ml. Slight cross reactions by chymosin, bovine, porcine and chicken pepsin did not affect the assay. Recovery of Fromase added to cheese extracts varied between 92 and 106%. The method was used for the determination of M. miehei proteinase in different cheese extracts.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 793 - 797
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

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