Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-03T02:42:48.958Z Has data issue: false hasContentIssue false

The relationship of bovine milk somatic cell count to neutrophil level in samples of cow's milk assessed by an automatic cell counter

Published online by Cambridge University Press:  08 July 2021

Zlatina Chengolova*
Affiliation:
Biotechnology Department, “Prof. Dr Asen Zlatarov” University of Burgas, Burgas, Bulgaria
Yavor Ivanov
Affiliation:
Biotechnology Department, “Prof. Dr Asen Zlatarov” University of Burgas, Burgas, Bulgaria
Galina Grigorova
Affiliation:
Biotechnology Department, “Prof. Dr Asen Zlatarov” University of Burgas, Burgas, Bulgaria
*
Author for correspondence: Zlatina Chengolova, Email: [email protected]

Abstract

This research communication describes the application of a fluorescent automatic cell counter Lactoscan SCC for simultaneous determination of somatic cell count and neutrophils in bovine milk. The obtained results were compared with results obtained by a flow cytometer and a light microscope. The Pearson correlations between the methods were calculated. A comparison between the main characteristics of the three kinds of analysis was made – the assay duration and the intra-assay precision. A relation between the SCC and neutrophil cells was observed in 55 milk samples. The obtained results confirm that the simultaneous determination of SCC and neutrophil analysis are necessary and support the early diagnosis of mastitis, the timely treatment of the animal and the avoidance of major economic losses.

Type
Research Article
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alhussien, M, Manjari, P, Sheikh, AA, Seman, SM, Reddi, S, Mohanty, AK and Dang, AK (2016) Immunological attributes of blood and milk neutrophils isolated from crossbred cows during different physiological conditions. Czech Journal of Animal Science 61, 223231.10.17221/63/2015-CJASCrossRefGoogle Scholar
Becheva, Z, Gabrovska, K and Godjevargova, T (2017) Immunofluorescence microscope assay of neutrophils and somatic cells in bovine milk. Food and Agricultural Immunology 28, 11961210.10.1080/09540105.2017.1332012CrossRefGoogle Scholar
Carrillo-Casas, EM and Miranda-Morales, RE (2012) Bovine mastitis pathogens: prevalence and effects on somatic cell count. Milk Production-An Up-to-Date Overview of Animal Nutrition, Management and Health 359-374 London UK IntechOpen. Editor Narongsak Chaiyabutr.Google Scholar
Commission Regulation (EC). No 1662/2006 of 6 November 2006 amending Regulation (EC) No 853/2004 of the European Parliament and of the Council laying down specific hygiene rules for food of animal origin (Text with EEA relevance). Available at https://op.europa.eu/en/publication-detail/-/publication/befe5c7a-c8a0-4518-8742-dc3a1b891836/language-enGoogle Scholar
Damm, M, Holm, C, Blaabjerg, M, Bro, MN and Schwarz, D (2017) Differential somatic cell count − A novel method for routine mastitis screening in the frame of Dairy Herd Improvement testing programs. Journal of Dairy Science 100, 49264940.CrossRefGoogle Scholar
Gargouri, A, Hamed, H and ElFeki, A (2008) Total and differential bulk cow milk somatic cell counts and their relation with lipolysis. Livestock Science 113, 274279.CrossRefGoogle Scholar
ISO 13366-1 (2008) [IDF 148-1:2008] Milk. Enumeration of somatic cells. Part 1: Microscopic method (Reference method). ICS : 67.100.10 Milk and processed milk products. Available at https://www.iso.org/standard/40259.htmlGoogle Scholar
ISO 707 (2008) Milk and milk products – Guidance on sampling. Available at https://www.iso.org/obp/ui/#iso:std:iso:707:ed-3:v1:enGoogle Scholar
Kelly, A, Tiernan, D, O'Sullivan, C and Joyce, P (2000) Correlation between bovine milk somatic cell count and polymorphonuclear leukocyte level for samples of bulk milk and milk from individual cows. Journal of Dairy Science 83, 300304.10.3168/jds.S0022-0302(00)74878-8CrossRefGoogle ScholarPubMed
Mehne, D, Drees, S, Schuberth, HJ and Sauter-Louis, C (2010) Accurate and rapid flow cytometric leukocyte differentiation in low and high somatic cell count milk. Milchwissenschaft 65, 235238.Google Scholar
Oliveira, L, Langoni, H, Hulland, C and Ruegg, PL (2012) Minimum inhibitory concentrations of Staphylococcus aureus recovered from clinical and subclinical cases of bovine mastitis. Journal of Dairy Science 95, 19131920.CrossRefGoogle ScholarPubMed
Schwarz, D, Diesterbeck, US, König, S, Brügemann, K, Schlez, K, Zschöck, M, Wolter, W and Czerny, CP (2011) Flow cytometric differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands. Journal of Dairy Science 10, 50335044.CrossRefGoogle Scholar
Schwarz, D, Lipkens, Z and Piepers, S (2019) Investigation of differential somatic cell count as a potential new supplementary indicator to somatic cell count for identification of intramammary infection in dairy cows at the end of the lactation period. Preventive Veterinary Medicine 172, 104803.CrossRefGoogle Scholar
Wald, R, Hess, C, Urbantke, V, Wittek, T and Baumgartner, M (2019) Characterization of Staphylococcus species isolated from bovine quarter milk samples. Animals 9, E200.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Chengolova et al. supplementary material

Chengolova et al. supplementary material

Download Chengolova et al. supplementary material(PDF)
PDF 516.8 KB