Hostname: page-component-cd9895bd7-fscjk Total loading time: 0 Render date: 2024-12-18T15:30:15.834Z Has data issue: false hasContentIssue false
  • English
  • Français

Recovery of milk fat globule membrane (MFGM) from buttermilk: effect of Ca-binding salts

Published online by Cambridge University Press:  23 August 2019

Vitaly L. Spitsberg ,
Liza Ivanov  and
Vladimir Shritz
Vitaly L. Spitsberg*
Affiliation:
Biovita Technologies, Bat Yam, Israel
Liza Ivanov
Affiliation:
AstraZeneca, Raanana, Israel
Vladimir Shritz
Affiliation:
Baemek Advanced Technology, Afula, Israel
*
Author for correspondence: Vitaly L. Spitsberg, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In this Research Communication we present a study of the effect of Ca-binding salts on the recovery of milk fat globule membrane (MFGM) from buttermilk. Sodium phosphate buffer was used for the purpose of MFGM recovery from buttermilk for the first time and we showed that 0.1 M buffer at pH 7.2 was the most effective for the recovery of MFGM. The fact of high efficacy of sodium phosphate buffer in recovery of MFGM from buttermilk allowed us to suggest that MFGM in buttermilk is present in association with casein through Ca- bridges formed between phospholipids of MFGM and phosphate groups of casein, primarily with k-casein as the peripheral protein of casein micelles.

Keywords

Buttermilkmilk fat globule membranephosphate buffer
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 3 , August 2019 , pp. 374 - 376
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Billeaud, C, Puccio, G, Salida, E, Guillois, B, Vaysse, C, Pecquet, S and Steenhout, PH (2014) Safety and tolerance evaluation of milk fat globule membrane-enriched infant formulas: a randomized controlled multicenter non-inferiority trial in healthy term infants. Clinical Medicine Insights: Pediatrics 8, 51–60.Google Scholar
Corredig, M, Roesch, RR and Dalgleish, DG (2003) Production of a novel ingredient from buttermilk. Journal of Dairy Science 86, 2744–2750.Google Scholar
Dalgleish, DG (1998) Casein micelles as colloids: surface structures and stabilities. Journal of Dairy Science 81, 3013–3018.Google Scholar
Fuller, KL, Kuhlenschmidt, TB, Kuhlenschmidt, MS, Jimenez-Flores, R and Donavan, R (2013) Milk fat globule membrane isolated from buttermilk or whey cream and their lipid components inhibit infectivity of rotavirus in vitro. Journal of Dairy Science 86, 2725–2743.Google Scholar
Hernell, O, Timby, N, Domellöf, M and Lönnerdal, B (2016) Clinical benefits of milk fat globule membranes for infants and children. Journal of Pediatrics 173, 60–65.Google Scholar
Holt, C, Davis, DT and Law, AJR (1986) Effects of colloidal calcium phosphate content and free calcium concentration in the milk serum on the dissociation of bovine casein micelles. Journal of Dairy Research 53, 557–572.Google Scholar
Holzmuller, W and Kulozik, U (2016) Quantification of MFGM proteins in buttermilk and butter serum by means a stain free SDS-PAGE method. Journal of Food Composition and Analysis 69, 102–109.Google Scholar
Mather, IM (2000) A review and proposed nomenclature for major proteins of the milk-fat globule membrane. Journal of Dairy Science 83, 203–247.Google Scholar
Snow, DR, Jimenez-Flores, R, Ward, RE, Cambell, J, Young, MJ, Nemere, I and Hintze, KJ (2010) Dietary milk fat globule membrane reduces the incidence of aberrant crypt foci in Fischer-344 rats. Journal of Agricultural and Food Chemistry 58, 2157–2163.Google Scholar
Spitsberg, VL (2005) Invited review: Bovine milk fat globule membrane as a potential nutraceutical. Journal of Dairy Science 88, 2289–2294.Google Scholar
Spitsberg, VL, Matitashvili, E and Gorewit, RC (1995) Association of fatty acid binding protein and glycoprotein CD36 in the bovine mammary gland. Journal of Biochemistry 230, 872–878.Google Scholar