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Quantitative fractionation of casein mixtures by fast protein liquid chromatography

Published online by Cambridge University Press:  01 June 2009

D. Thomas Davies  and
Andrew J. R. Law
D. Thomas Davies
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
Andrew J. R. Law
Affiliation:
Hannah Research Institute, Ayr KA6 5HL, UK
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Summary

Alkylation of whole casein samples by reaction with cysteamine and cystamine in a bis-tris-propane–urea buffer (pH 7·0) followed by fast protein liquid chromatography (FPLC) at 20°C on a Mono Q HR5/5 column in the same buffer and using a NaCl gradient led to good resolution of the whole casein into fractions representing (i) γ2- plus γ3-caseins, (ii) κ-caseins, (iii) β-casein, (iv) αs2-caseins and (v) αsl-caseins, together with small amounts of unidentified materials. Quantitatively the FPLC values agreed well with those for αs1-, β-, αs2- and γ2- plus γ3-caseins obtained by ion-exchange chromatography on DEAE cellulose, Whatman DE52 and with those for º-caseins obtained by gel-permeation chromatography on Sephadex G–150.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 3 , August 1987 , pp. 369 - 376
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

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