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Proteomic profiling of ovine milk after grading up

Published online by Cambridge University Press:  14 May 2021

Xiaohu Su ,
Zhong Zheng ,
Liguo Zhang ,
Urhan Bai ,
Ying Ma ,
Yingjie Dou ,
Xiaoran Zhang ,
Guanghua Su ,
Ningcong Zhou  and
Guangpeng Li
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Xiaohu Su*
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Zhong Zheng
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Liguo Zhang
Affiliation:
Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Ulanqab, Inner Mongolia Autonomous Region, 012000, PR China
Urhan Bai
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Ying Ma
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Yingjie Dou
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Xiaoran Zhang
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Guanghua Su
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Ningcong Zhou
Affiliation:
Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Ulanqab, Inner Mongolia Autonomous Region, 012000, PR China
Guangpeng Li
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Li Zhang*
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
*
Author for correspondence: Li Zhang, Email: [email protected]; Xiaohu Su, Email: [email protected]
Author for correspondence: Li Zhang, Email: [email protected]; Xiaohu Su, Email: [email protected]
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Abstract

We have previously bred Chinese local dairy sheep through grading up with local Small-Tailed Han (STH) sheep as female parent and DairyMeade (DM) sheep as male parent. In this research communication we characterize the whey protein profile of STH sheep and their offspring (F1, F2) to reveal physiological differences and variation in milk traits. A total of 1032 whey proteins were identified through tandem mass tag labeling (TMT) proteome profiling. Three proteins were significantly differentially abundant between F1 and STH milk, six between F2 and STH milk and five between F1 and F2 milk. In terms of differential changes between generations, WASHC4 and CUTA of F1 and Ig-like domain-containing protein of F2 milk were dominant whey proteins. Overall, the results showed that the whey protein profiles of different generations varied little. The crossbreeds of STH and DM sheep would be suitable for the development of the Chinese local sheep milk industry, and the F2 may be a better population for sheep milk production.

Keywords

Milkovineproteomic
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 2 , May 2021 , pp. 201 - 204
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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Footnotes

*

These authors contributed equally to this project.

References

Anagnostopoulos, AK, Katsafadou, AI, Pierros, V, Kontopodis, E, Fthenakis, GC, Arsenos, G, Karkabounas, SC, Tzora, A, Skoufos, I and Tsangaris, GT (2016) Milk of Greek sheep and goat breeds; characterization by means of proteomics. Journal of Proteomics 147, 76–84.CrossRefGoogle ScholarPubMed
Chen, Q, Ke, X, Zhang, JS, Lai, SY, Fang, F, Mo, WM and Ren, YP (2016) Proteomics method to quantify the percentage of cow, goat, and sheep milks in raw materials for dairy products. Journal of Dairy Science 99, 9483–9492.Google ScholarPubMed
de Groot, N, van Kuik-Romeijn, P, Lee, SH and de Boer, HA (2001) Over-expression of the murine pIgR gene in the mammary gland of transgenic mice influences the milk composition and reduces its nutritional value. Transgenic Research 10, 285–291.CrossRefGoogle ScholarPubMed
Ferro, MM, Tedeschi, LO and Atzori, AS (2017) The comparison of the lactation and milk yield and composition of selected breeds of sheep and goats. Translational Animal Science 1, 498–506.CrossRefGoogle ScholarPubMed
Greco, E, El-Aguizy, O, Ali, MF, Foti, S, Cunsolo, V, Saletti, R and Ciliberto, E (2018) Proteomic analyses on an ancient Egyptian cheese and biomolecular evidence of brucellosis. Analytical Chemistry 90, 9673–9676.CrossRefGoogle Scholar
Hou, P, Liu, G, Zhao, Y, Shi, Z, Zheng, Q, Bu, G, Xu, H and Zhang, YW (2015) Role of copper and the copper-related protein CUTA in mediating APP processing and Aβ generation. Neurobiology of Aging 36, 1310–1315.CrossRefGoogle ScholarPubMed
King, ME, King, JE and Powell, CB (2014) Sheep dairying in New Zealand – the Kingsmeade story. Proceedings of the New Zealand Society of Animal Production 74, 58–61.Google Scholar
Kustermann, M, Manta, L, Paone, C, Kustermann, J, Lausser, L, Wiesner, C, Eichinger, L, Clemen, CS, Schröder, R, Kestler, HA, Sandri, M, Rottbauer, W and Just, S (2018) Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo. Autophagy 14, 1911–1927.CrossRefGoogle ScholarPubMed
Lönnerdal, B (2014) Infant formula and infant nutrition: bioactive proteins of human milk and implications for composition of infant formulas. The American Journal of Clinical Nutrition 99, 712s–717s.CrossRefGoogle ScholarPubMed
Lu, Y, Wang, WS, Lin, YK, Lu, JW, Li, WJ, Zhang, CY and Sun, K (2019) Enhancement of cortisol-induced SAA1 transcription by SAA1 in the human amnion. Journal of Molecular Endocrinology 62, 149–158.CrossRefGoogle ScholarPubMed
Pietrzak-Fiećko, R and Kamelska-Sadowska, AM (2020) The comparison of nutritional value of human milk with other mammals' milk. Nutrients 12, 1404.CrossRefGoogle ScholarPubMed
Reznikov, EA, Comstock, SS, Yi, C, Contractor, N and Donovan, SM (2014) Dietary bovine lactoferrin increases intestinal cell proliferation in neonatal piglets. The Journal of Nutrition 144, 1401–1408.CrossRefGoogle ScholarPubMed
Tomazou, M, Oulas, A, Anagnostopoulos, AK, Tsangaris, GT and Spyrou, GM (2019) In silico identification of antimicrobial peptides in the proteomes of goat and sheep milk and Feta cheese. Proteomes 7, 32.CrossRefGoogle ScholarPubMed
Wang, J, Zhou, H, Hickford, JGH, Hao, Z, Shen, J, Luo, Y, Hu, J, Liu, X and Li, S (2020) Comparison of the transcriptome of the ovine mammary gland in lactating and non-lactating small-tailed Han sheep. Frontiers in Genetics 11, 472.CrossRefGoogle ScholarPubMed
Yang, S, Gao, Y, Zhang, S, Zhang, Q and Sun, D (2016) Identification of genetic associations and functional polymorphisms of SAA1 gene affecting milk production traits in dairy cattle. PLoS One 11, e0162195.Google ScholarPubMed
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