Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-27T03:56:08.817Z Has data issue: false hasContentIssue false
  • English
  • Français

Protein degradation in bovine milk caused by Streptococcus agalactiae

Published online by Cambridge University Press:  01 August 2012

Maria Åkerstedt ,
Ewa Wredle ,
Vo Lam  and
Monika Johansson
Maria Åkerstedt*
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, SE-750 07, Uppsala, Sweden
Ewa Wredle
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, SE-750 07, Uppsala, Sweden
Vo Lam
Affiliation:
Department of Animal Nutrition and Management, Swedish University of Agricultural Sciences, SE-750 07, Uppsala, Sweden Department of Animal Husbandry and Veterinary Sciences, Angiang University, An Giang Province, Vietnam
Monika Johansson*
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Streptococcus (Str.) agalactiae is a contagious mastitis bacterium, often associated with cases of subclinical mastitis. Different mastitis bacteria have been evaluated previously from a diagnostic point of view, but there is a lack of knowledge concerning their effect on milk composition. Protein composition is important in achieving optimal yield and texture when milk is processed to fermented products, such as cheese and yoghurt, and is thus of great economic value. The aim of this in vitro study was to evaluate protein degradation mainly caused by exogenous proteases originating from naturally occurring Str. agalactiae. The samples were incubated at 37°C to imitate degradation caused by the bacteria in the udder. Protein degradation caused by different strains of Str. agalactiae was also investigated. Protein degradation was observed to occur when Str. agalactiae was added to milk, but there were variations between strains of the bacteria. Caseins, the most economically important proteins in milk, were degraded up to 75% in milk inoculated with Str. agalactiae in relation to sterile ultra-high temperature (UHT) milk, used as control milk. The major whey proteins, α-lactalbumin and β-lactoglobulin, were degraded up to 21% in relation to the sterile control milk. These results suggest that different mastitis bacteria but also different strains of mastitis bacteria should be evaluated from a milk quality perspective to gain knowledge about their ability to degrade the economically important proteins in milk.

Keywords

MastitisCapillary electrophoresisin vitromilk protein
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 3 , August 2012 , pp. 297 - 303
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Artursson, K, Nilsson-Öst, M & Persson Waller, K 2010 An improved method to culture Staphylococcus aureus from bovine milk. Journal of Dairy Science 93 1534–1538 CrossRefGoogle ScholarPubMed
Auldist, MJ, Coats, S, Sutherland, BJ, Mayes, JJ, McDowell, GH & Rogers, GL 1996 Effects of somatic cell count and stage of lactation on raw milk composition and the yield and quality of Cheddar cheese. Journal of Dairy Research 63 269–280 CrossRefGoogle ScholarPubMed
Bastian, ED & Brown, RJ 1996 Plasmin in milk and dairy products: an update. International Dairy Journal 6 435–457 CrossRefGoogle Scholar
Bertrand-Harb, C, Ivanova, IV, Dalgalarrondo, M & Haertllé, T 2003 Evolution of β-lactoglobulin and α-lactalbumin content during yoghurt fermentation. International Dairy Journal 13 39–45 CrossRefGoogle Scholar
Bradley, AJ 2002 Bovine mastitis: An evolving disease. Veterinary Journal 164 116–128 CrossRefGoogle ScholarPubMed
Datta, N & Deeth, HC 2003 Diagnosing the cause of proteolysis in UHT milk. LWT – Food Science and Technology 36 173–182 CrossRefGoogle Scholar
Dufour, D, Jameh, N, Dary, A & Le Roux, Y 2009 Short communication: Can the mammopathogenic Escherichia coli P4 strain have a direct role on the caseinolysis of milk observed during bovine mastitis? Journal of Dairy Science 92 1398–1403 CrossRefGoogle ScholarPubMed
Eriksson Unnerstad, H, Lindberg, A, Persson Waller, K, Ekman, T, Artursson, K, Nilsson-Ost, M & Bengtsson, B 2009 Microbial aetiology of acute clinical mastitis and agent-specific risk factors. Veterinary Microbiology 137 90–97 CrossRefGoogle Scholar
Fairbairn, DJ & Law, BA 1986 Proteinases of psychrotrophic bacteria: their production, properties, effects and control. Journal of Dairy Research 53 139–177 CrossRefGoogle ScholarPubMed
Fajardo-Lira, C, Oria, M, Hayes, KD & Nielsen, SS 2000 Effect of psychrotrophic bacteria and of an isolated protease from Pseudomonas fluorescens M3/6 on the plasmin system of fresh milk. Journal of Dairy Science 83 2190–2199 CrossRefGoogle ScholarPubMed
Farrell, HM, Malin, EL, Brown, EM & Mora-Gutierrez, A 2009 Review of the chemistry of αS2-casein and the generation of a homologous molecular model to explain its properties. Journal of Dairy Science 92 1338–1353 CrossRefGoogle ScholarPubMed
Fasola, E, Livdahl, C & Ferrieri, P 1993 Molecular analysis of multiple isolates of the major serotypes of group B streptococci . Journal of Clinical Microbiology 31 2616–2620 CrossRefGoogle ScholarPubMed
Grufferty, MB & Fox, PF 1988 Milk alkaline proteinase. Journal of Dairy Research 55 609–630 CrossRefGoogle ScholarPubMed
Haddadi, K, Moussaoui, F, Hebia, I, Laurent, F & Le Roux, Y 2005 E. coli proteolytic activity in milk and casein breakdown. Reproduction Nutrition Development 45 485–496 CrossRefGoogle ScholarPubMed
Haddadi, K, Prin-Mathieu, C, Moussaoui, F, Faure, GC, Vangroenweghe, F, Burvenich, C & Le Roux, Y 2006 Polymorphonuclear neutrophils and Escherichia coli proteases involved in proteolysis of casein during experimental E. coli mastitis. International Dairy Journal 16 639–647 CrossRefGoogle Scholar
Heck, JML, Olieman, C, Schennink, A, van Valenberg, HJF, Visker, MHPW, Meuldijk, RCR & van Hooijdonk, ACM 2008 Estimation of variation in concentration, phosphorylation and genetic polymorphism of milk proteins using capillary zone electrophoresis. International Dairy Journal 18 548–555 CrossRefGoogle Scholar
Hirai, Y, Permyakov, EA & Berliner, LJ 1992 Proteolytic digestion of alpha-lactalbumin: Physiological implications. Journal of Protein Chemistry 11 51–57 CrossRefGoogle ScholarPubMed
Holt, C 1992 Structure and stability of bovine casein micelles. Advances in Protein Chemistry 43 63–153 CrossRefGoogle ScholarPubMed
Jeffrey, DC & Wilson, J 1987 Effect of mastitis-related bacteria on total bacterial count of bulk milk supplies. International Journal of Dairy Technology 40 23–26 CrossRefGoogle Scholar
Keefe, GP 1997 Streptococcus agalactiae mastitis: A review. Canadian Veterinary Journal 38 429–437 Google ScholarPubMed
Kelly, AL, O'Flaherty, F & Fox, PF 2006 Indigenous proteolytic enzymes in milk: A brief overview of the present state of knowledge. International Dairy Journal 16 563–572 CrossRefGoogle Scholar
Kitchen, BJ 1981 Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. Journal of Dairy Research 48 167–188 CrossRefGoogle ScholarPubMed
Lam, V, Östensson, K, Svennersten-Sjaunja, K, Norell, L & Wredle, E 2011 Management factors influencing milk somatic cell count and udder infection rate in smallholder dairy cow herds in Southern Vietnam. Journal of Animal and Veterinary Advances 10 847–852 Google Scholar
Larsen, LB, Rasmussen, MD, Bjerring, M & Nielsen, JH 2004 Proteases and protein degradation in milk from cows infected with Streptococcus uberis . International Dairy Journal 14 899–907 CrossRefGoogle Scholar
Larsen, LB, McSweeney, PLH, Hayes, MG, Andersen, JB, Ingvartsen, KL & Kelly, AL 2006 Variation in activity and heterogeneity of bovine milk proteases with stage of lactation and somatic cell count. International Dairy Journal 16 1–8 CrossRefGoogle Scholar
Le Roux, Y, Laurent, F & Moussaoui, F 2003 Polymorphonuclear proteolytic activity and milk composition change. Veterinary Research 34 629–645 CrossRefGoogle ScholarPubMed
Leitner, G, Krifucks, O, Merin, U, Lavi, Y & Silanikove, N 2006 Interactions between bacteria type, proteolysis of casein and physico-chemical properties of bovine milk. International Dairy Journal 16 648–654 CrossRefGoogle Scholar
Merin, U, Fleminger, G, Komanovsky, J, Silanikove, N, Bernstein, S & Leitner, G 2008 Sub-clinical udder infection with Streptococcus dysgalactiae impairs milk coagulation properties: the emerging role of proteose peptones. Dairy Science Technology 88 407–419 CrossRefGoogle Scholar
Miralles, B, Ramos, M & Amigo, L 2003 Influence of proteolysis of milk on the whey protein to total protein ratio as determined by capillary electrophoresis. Journal of Dairy Science 86 2813–2817 CrossRefGoogle ScholarPubMed
Murphy, SC, Cranker, K, Senyk, GF, Barbano, DM, Saeman, AI & Galton, DM 1989 Influence of bovine mastitis on lipolysis and proteolysis in milk. Journal of Dairy Science 72 620–626 CrossRefGoogle ScholarPubMed
Ng-Kwai-Hang, KF 2002 Milk proteins heterogeneity, fractionation and isolation. Encyclopedia of Dairy Sciences Volume 1, second edition 1881–1894 CrossRefGoogle Scholar
Olde Riekerink, RGM, Barkema, HW, Veenstra, S, Poole, DE, Dingwell, RT & Keefe, GP 2006 Prevalence of contagious mastitis pathogens in bulk tank milk in Prince Edward Island. Canadian Veterinary Journal 47 567–572 Google ScholarPubMed
Saeman, AI, Verdi, RJ, Galton, DM & Barbano, DM 1988 Effect of mastitis on proteolytic activity in bovine milk. Journal of Dairy Science 71 505–512 CrossRefGoogle ScholarPubMed
Schmidt, DG & Markwijk, BW 1993 Enzymatic hydrolysis of whey proteins. Influence of heat treatment of α-lactalbumin and β-lactoglobulin on their proteolysis by pepsin and papain. Netherlands Milk and Dairy Journal 47 15–22 Google Scholar
Sørhaug, T & Stepaniak, L 1997 Psychrotrophs and their enzymes in milk and dairy products: Quality aspects. Trends in Food Science and Technology 8 35–41 CrossRefGoogle Scholar