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Para-κ-casein during the ripening and storage of low-pH, high-moisture Feta cheese

Published online by Cambridge University Press:  22 May 2018

Voula Alexandraki  and
Golfo Moatsou
Voula Alexandraki
Affiliation:
Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece
Golfo Moatsou*
Affiliation:
Laboratory of Dairy Research, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece
*
*For correspondence; e-mail: [email protected]
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Abstract

The hypothesis of this research paper was that the physicochemical conditions in a low-pH, high-moisture white brined cheese such as Feta would make para-κ-casein vulnerable to residual chymosin activity during ripening and storage. It was important to address this hypothesis, since cheese para-κ-casein could theoretically be used for the assessment of the origin of cheese milk by means of various analytical methods. Feta cheese was manufactured from sheep milk and from four different mixtures of sheep and goat milk in triplicate. The para-κ-casein of Feta samples taken during 120 d of ripening and storage was estimated by means of cation-exchange HPLC and proteolysis was determined in terms of free amino groups. Despite their similarity, sheep and goat para-κ-casein were efficiently separated and the changes of their chromatographic areas indicated that hydrolysis took place during the first stage of ripening. In accordance to the evolution of free amino groups, para-κ-caseins remained stable thereafter. The hydrolysis pattern was not affected by the composition of the cheese milk mixture and after 120 d at least two thirds of the initial quantity remained intact. Considering the efficient separation of sheep and goat para-κ-caseins and their stability during Feta storage, the same method was used for the evaluation of the percentage of goat milk in the cheese milk. The actual and the estimated percentage of goat milk within the range 0–40 were strongly correlated (R = 0·997, n = 60) and the standard error of estimation was 0·914.

Keywords

Para-κ-caseinhydrolysissheep and goat cheese milkFeta
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 226 - 231
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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References

Addeo, F, Moio, L, Chianese, L & Stingo, C 1990 Improved procedure for detecting bovine and ovine milk mixtures in cheese by isoelectric focusing of para-κ-casein. Milchwissenschaft 45 221–224Google Scholar
Coll, A, Folch, JM & Sànchez, A 1993 Rapid communication: nucleotide sequence of the goat κ-casein cDNA. Journal of Animal Science 71 2833Google Scholar
Coolbear, KP, Elgar, DF, Coolbear, T & Ayers, JS 1996 Comparative study of methods for the isolation and purification of bovine κ-casein and its hydrolysis by chymosin. Journal of Dairy Research 63 61–71CrossRefGoogle ScholarPubMed
de Roos, AHL, Geurts, TJ & Walstra, P 2000 The association of chymosin with artificial casein micelles. International Dairy Journal 10 225–232Google Scholar
Ferranti, P, Itolli, E, Barone, F, Malorni, A, Garro, G, Laezza, P, Chianese, L, Migliaccio, F, Stingo, V & Addeo, F 1997 Combined high resolution chromatographic techniques (FPLC and HPLC) and mass spectrometry-based identification of peptides and proteins in Grana Padano cheese. Le Lait 81 463–474Google Scholar
Furet, JP, Mercier, JC, Soulier, S, Gaye, P, Hue-Delahaie, D & Vilotte, JL 1990 Nucleotide sequence of ovine κ-casein. Nucleic Acids Research 18 5286Google Scholar
Juan, B, Zamora, A, Quevedo, JM & Trujillo, A-J 2016 Proteolysis of cheese made from goat milk treated by ultra high pressure homogenization. LWT – Food Science and Technology 69 17–23Google Scholar
Larsson, KI & Andrén, A 1997 Affinity between chymosin and individual caseins at varying pH-values. International Dairy Journal 7 615–618Google Scholar
Mallatou, H & Pappa, EC 2005 Comparison of the characteristics of teleme cheese made from ewe's, goat's and cow's milk or a mixture of ewe's and goat's milk. International Journal of Dairy Technology 58 158–163Google Scholar
Mallatou, H, Pappa, EC & Boumba, VA 2004 Proteolysis in Teleme cheese made from sheep's, goat's or a mixture of sheep's and goat's milk. International Dairy Journal 14 977–987Google Scholar
Mayer, HK 2005 Milk species identification in cheese varieties using electrophoretic, chromatographic and PCR techniques. International Dairy Journal 15 595–604CrossRefGoogle Scholar
Mayer, HK, Heidler, D & Rockenbauer, C 1997 Determination of the percentages of cows’, ewes’ and goats’ milk in cheese by isoelectric focusing and cation-exchange HPLC of γ- and para-κ-caseins. International Dairy Journal 7 619–628Google Scholar
McSweeney, PLH 2004 Biochemistry of cheese ripening: introduction and overview. In General Aspects: Vol. 1. Cheese Chemistry, Physics and Microbiology, pp. 347–360 (Eds Fox, PF, McSweeney, PLH, Cogan, TM & Guinee, TP) Amsterdam, The Netherlands: Elsevier Academic PressGoogle Scholar
Michaelidou, A, Alichanidis, E, Urlaub, H, Polychroniadou, A & Zerfiridis, GK 1998 Isolation and identification of some major water-soluble peptides in Feta cheese. Journal of Dairy Science 81 3109–3116Google Scholar
Moatsou, G & Govaris, A 2011 White brined cheeses: a diachronic exploitation of small ruminants milk in Greece. Small Ruminants Research 101 113–121CrossRefGoogle Scholar
Moatsou, G, Hatzinaki, A, Psathas, G & Anifantakis, E 2004 Detection of caprine casein in ovine Halloumi cheese. International Dairy Journal 14 219–226CrossRefGoogle Scholar
Nega, A & Moatsou, G 2012 Proteolysis and related enzymatic activities in ten Greek cheese varieties. Dairy Science and Technology 92 57–73CrossRefGoogle Scholar
Perna, A, Simonetti, A, Intaglietta, I & Gambacorta, E 2014 Effects of genetic type stage of lactation and ripening time on Caciocavallo cheese proteolysis. Journal of Dairy Science 97 1909–1917Google Scholar
Polychroniadou, A 1988 A simple procedure using trinitrobenzenesulphonic acid for monitoring proteolysis in cheese. Journal of Dairy Research 55 585–596Google Scholar
Reid, JR, Coolbear, T, Ayers, JS & Coolbear, KP 1997 The action of chymosin on κ-casein and its macropeptide: effect of pH and analysis of the products of secondary hydrolysis. International Dairy Journal 7 559–569Google Scholar
Tsartsianidou, V, Triantafillidou, D, Karaiskou, N, Tarantili, P, Triantafillidis, G, Georgakis, E & Triantafyllidis, A 2017 Caprine and ovine Greek dairy products: the official German method generates false-positive results due to κ-casein gene polymorphism. Journal of Dairy Science 100 3539–3547CrossRefGoogle ScholarPubMed
Zoidou, E, Plakas, N, Giannopoulou, D, Kotoula, M & Moatsou, G 2015 Effect of supplementation of brine with calcium on the evolution of Feta ripening. International Journal of Dairy Technology 658 420–426Google Scholar