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On-farm use of a water hardness test kit to assess total blood calcium level in dairy cattle

Published online by Cambridge University Press:  03 February 2020

Filippo Fiore ,
Raffaella Cocco ,
Daniele Musina  and
Nicoletta Spissu
Filippo Fiore*
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100Sassari, Italy
Raffaella Cocco
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100Sassari, Italy
Daniele Musina
Affiliation:
Loc. Perdas Arbas, 08100Nuoro, Italy
Nicoletta Spissu
Affiliation:
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100Sassari, Italy
*
Author for correspondence: Filippo Fiore, Email: [email protected]
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Abstract

In this Research Communication we report the measurement of total blood calcium concentration with a water hardness test kit (WHTK) in dairy cattle and compare the results to a reference method. The study was carried out on 107 Holstein Friesian cows from three commercial dairy farms in Sardinia (Italy). Blood samples were collected ≤7 d from calving and total serum calcium concentration was determined using a biochemical analyzer and a WHTK. The relationship between the laboratory results and the WHTK results was investigated using Spearman's rank correlation test and simple linear regression was determined. Sensitivity, specificity, negative and positive predictive values, intra and inter-assay coefficient of variation were also calculated. The test values were highly correlated with the laboratory values (R2 = 0.72, P < 0.001). Sensitivity and specificity were respectively 88 and 83% for the WHTK and intra and inter-assay coefficients of variation were 7.3 and 11.3% respectively. The WHTK was identified as a potential on-farm tool for monitoring early postpartum Ca concentrations at herd level.

Keywords

Dairy cowdiagnostic kithypocalcemia
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 56 - 59
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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