Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T22:54:11.545Z Has data issue: false hasContentIssue false
  • English
  • Français

New αs2-casein variant from caprine milk

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Rosalba Mauriello ,
Nunziatina Intorcia ,
Luigi Moio  and
Francesco Addeo
Lina Chianese
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Rosalba Mauriello
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Nunziatina Intorcia
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Luigi Moio
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Francesco Addeo
Affiliation:
Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici (NA), Italia
Get access Rights & Permissions [Opens in a new window]

Summary

A new αs2-casein variant was isolated from goat milk by means of covalent chromatography. With gel electrophoresis at alkaline pH, the αs2-casein variant showed a double band with the highest anodic mobility amongst the casein components. The mobility of the isolated αs2-casein variant was similar to that occurring in three individual samples of whole casein. Two dimensional electrophoresis revealed a more pronounced heterogeneity of this protein. Developing the two dimensional plate with polyclonal antibodies obtained against the four main bovine casein fractions, we demonstrated that the variant in question belongs to the αs2-casein family. Since the αs2-casein fractions, immunospecifically developed, spread along two different zones on the gel, it was concluded that a heterozygous αs2-casein form was present in the sample.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 3 , August 1992 , pp. 299 - 305
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Addeo, F., Mauriello, R., & Di Luccia, A. 1988. A gel electrophoretic study of caprine casein. Journal of Dairy Research 55 413–421CrossRefGoogle Scholar
Aschaffenburg, R. & Drewry, J. 1959 New procedure for the routine determination of the various non-casein proteins of milk. 15th International Dairy Congress, London 3 1631–1637Google Scholar
Blakesley, R. W. & Boezi, J. A. 1977 A new staining technique for proteins in polyacrylamide gels using Coomassie brilliant blue G250. Analytical Biochemistry 82 580–582CrossRefGoogle ScholarPubMed
Boulanger, A., Grosclaude, F. & Mahé, M. F. 1984 [Caprine αs1- and αs2-casein polymorphism (Capra hircus).] Génétique, Sélection, Évolution 16 157–176Google Scholar
Brignon, G., Ribadeau Dumas, B., Mercier, J. C., Pélissier, J. P. & Das, B. C. 1977 Complete amino acid sequence of bovine αs2-casein. FEBS Letters 76 274–279CrossRefGoogle ScholarPubMed
Chianese, L., D'Acierno, C., Mauriello, R., Nizza, A., Di Meo, C. & Luongo, D. 1990 [Milk casein composition in caprine breed reared in Campania.] Zootecnica e Nutrizione Animale 16 419–425Google Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1992 Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques. Journal of Dairy Research 59 39–47Google Scholar
Grosclaude, F., Mahé, M.-F., Brignon, G., Di Stasio, L. & Jeunet, R. 1987 A Mendelian polymorphism underlying quantitative variations of goat αs1-casein. Génétique, Sélection, Évolution 19 399–411Google Scholar
Mercier, J. C., Maubois, J. L., Poznańzki, S. & Ribadeau Dumas, B. 1968 [Preparative fractionation of bovine and ovine casein by DEAE-cellulose chromatography in a medium containing urea and 2- mercaptoethanol.] Bulletin de la Société de Chimie Biologigue 50 521–530Google Scholar
Mercier, J. C., Grosclaude, F. & Ribadeau Dumas, B. 1971 [Primary structure of bovine αs1-casein. Complete sequence.] European Journal of Biochemistry 23 41–51Google Scholar
Moio, L., Chianese, L., Di Luccia, A., Mauriello, R., Stingo, C. & Addeo, F. 1989 Identification of casein variants by immunoblotting. In Electrophoresis Forum ′89 pp. 475–478 (Ed. Radola, B. J.). MünchenGoogle Scholar
Nijhuis, H. & Kostermeyer, H. 1975 Partial fractionation of whole casein by affinity chromatography. Milchwissenschaft 30 530–531Google Scholar
Trieu-Cuot, P. & Gripon, J. C. 1981 Electrofocusing and two-dimensional electrophoresis of bovine caseins. Journal of Dairy Research 48 303–310CrossRefGoogle Scholar