Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-08T12:23:54.024Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular characterisation of the buffalo SCAP gene and its association with milk production traits in water buffaloes

Published online by Cambridge University Press:  22 May 2018

Tingxian Deng ,
Xiaoya Ma ,
Chunying Pang ,
Shasha Liang ,
Xingrong Lu ,
Anqin Duan  and
Xianwei Liang
Tingxian Deng
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Xiaoya Ma
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Chunying Pang
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Shasha Liang
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Xingrong Lu
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Anqin Duan
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
Xianwei Liang*
Affiliation:
Guangxi provincial Key Laboratory of Buffalo Genetics, Breeding and Reproduction technology, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The study reported in this Research Communication was conducted to investigate the molecular characterisation of buffalo SCAP gene, expression analysis, and the association between single nucleotide polymorphisms and milk production traits in 384 buffaloes. Sequence analysis revealed the SCAP gene had an open reading frame of 3837 bp encoding 1279 amino acids. A ubiquitous expression profile of SCAP gene was detected in various tissues with extreme predominance in the mammary gland during early lactation. Moreover, eleven SNPs in buffalo SCAP gene were identified, six of them (g.1717600A>G, g.1757922C>T, g.1758953G>A, g.1759142C>T, g.1760740G>A, and g.1766036T>C) were found to be significantly associated with 305-day milk yield. Thus, buffalo SCAP could sever as a candidate gene affecting milk production traits in buffalo and the identified SNPs might potentially be genetic markers.

Keywords

Buffalomilk production traitsSCAPsingle nucleotide polymorphism
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 2 , May 2018 , pp. 133 - 137
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aspilcueta-Borquis, RR, Sesana, RC, Berrocal, MH, de Oliveira Seno, L, Bignardi, AB, Faro, LE, de Albuquerque, LG, de Camargo, GM & Tonhati, H 2010 Genetic parameters for milk, fat and protein yields in Murrah buffaloes (Bubalus bubalis Artiodactyla, Bovidae). Genetics and Molecular Biology 33 71–77Google Scholar
Bionaz, M & Loor, JJ 2008 Gene networks driving bovine milk fat synthesis during the lactation cycle. BMC Genomics 9 366Google Scholar
Cao, H, Miskie, BA & Hegele, RA 2002 Functional promoter polymorphism in SREBP cleavage-activating protein (SCAP). Journal of Human Genetics 47 492–496Google Scholar
Deng, T, Pang, C, Ma, X, Lu, X, Duan, A, Zhu, P & Liang, X 2016 Four novel polymorphisms of buffalo INSIG2 gene are associated with milk production traits in Chinese buffaloes. Molecular and Cellular Probes 30 294–299Google Scholar
Dixit, SP, Sivalingam, J, Tyagi, AK, Saroha, V, Sharma, A & Nagda, RK 2015 Association of novel SNPs in the candidate genes affecting caprine milk fatty acids related to human health. Meta Gene 4 45–56Google Scholar
Iwaki, K, Nakajima, T, Ota, N & Emi, M 1999 A common Ile796Val polymorphism of the human SREBP cleavage-activating protein (SCAP) gene. Journal of Human Genetics 44 421–422Google Scholar
Liu, FH, Song, JY, Shang, XR, Meng, XR, Ma, J & Wang, HJ 2014 The gene-gene interaction of INSIG-SCAP-SREBP pathway on the risk of obesity in Chinese children. BioMed Resarch International 2014 538564Google Scholar
Luskey, KL & Stevens, B 1985 Human 3-hydroxy-3-methylglutaryl coenzyme A reductase. Conserved domains responsible for catalytic activity and sterol-regulated degradation. Journal of Biological Chemistry 260 10271–10277Google Scholar
Qiu, H, Xia, T, Chen, X, Zhao, X, Gan, L, Feng, S, Lei, T & Yang, Z 2006 Cloning, comparative characterization of porcine SCAP gene, and identification of its two splice variants. Molecular Genetics and Genomics 276 187–196Google Scholar
Rincon, G, Islas-Trejo, A, Castillo, AR, Bauman, DE, German, BJ & Medrano, JF 2012 Polymorphisms in genes in the SREBP1 signalling pathway and SCD are associated with milk fatty acid composition in Holstein cattle. Journal of Dairy Research 79 66–75Google Scholar
Seegmiller, AC, Dobrosotskaya, I, Goldstein, JL, Ho, YK, Brown, MS & Rawson, RB 2002 The SREBP pathway in drosophila: regulation by palmitate, not sterols. Developmental Cell 2 229–238CrossRefGoogle Scholar
Stirnimann, CU, Petsalaki, E, Russell, RB & Muller, CW 2010 WD40 proteins propel cellular networks. Trends in Biochemical Science 35 565–574Google Scholar
Swalve, HH 1995 The effect of test day models on the estimation of genetic parameters and breeding values for dairy yield traits. Journal of Dairy Science 78 929–938Google Scholar
Weber, LW, Boll, M & Stampfl, A 2004 Maintaining cholesterol homeostasis: sterol regulatory element-binding proteins. World Journal of Gastroenterol 10 3081–3087Google Scholar
Supplementary material: PDF

Deng et al. supplementary material

Tables S1-S3 and Figures S1-S3

Download Deng et al. supplementary material(PDF)
PDF 1.1 MB