Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-09T22:59:38.522Z Has data issue: false hasContentIssue false
  • English
  • Français

Model proteolysis of β-casein by immobilized trypsin

Published online by Cambridge University Press:  01 June 2009

Ernst H. Reimerdes
Ernst H. Reimerdes
Affiliation:
Institute for Chemistry, Federal Dairy Research Centre, D 2300 Kiel, Federal Republic of Germany
Get access Rights & Permissions [Opens in a new window]

Summary

The enzymic degradation of β-casein by immobilized trypsin is described and its use as a model system to study proteolytic reactions which occur in milk during processing is examined. Quantitative determination of proteolysis can be obtained by densitometric transmission measurement of patterns obtained by polyacrylamide-gel electrophoresis. Staining properties of some of the individual components in the electrophoretic patterns were determined by calibration with pure protein and peptide preparations. The isolation of pure β- and γ-caseins and the preparation of several forms of immobilized trypsin are outlined.

Type
Section B. Milk Proteins: Comparative Aspects, and the Study of some of the Minor Caseins
Information
Journal of Dairy Research , Volume 46 , Issue 2: Symposium on the Physics and Chemistry of Milk Proteins , April 1979 , pp. 223 - 226
Copyright
Copyright © Proprietors of Journal of Dairy Research 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aschaffenburg, R. (1963). Journal of Dairy Research 30, 259.CrossRefGoogle Scholar
Eigel, W. N. (1977). Journal of Biochemistry 8, 178.Google Scholar
Garnier, J., Ribadeau Dumas, B. & Mocquot, G. (1964). Journal of Dairy Research 31, 131.CrossRefGoogle Scholar
Jenness, R. & Koops, J. (1962). Netherlands Milk and Dairy Journal 16, 153.Google Scholar
Kamtnogawa, S., Mizobuchi, H. & Yamauchi, K. (1972). Agricultural and Biological Chemistry (Japan) 36, 12.Google Scholar
Mercier, M., Grosclaude, F. & Ribadeau Dumas, B. (1972). Milchwissenschaft 27, 402.Google Scholar
Mikami, M. (1971). Japanese Journal of Zootechnological Science 42, 281.Google Scholar
Reimerdes, E. H. & Klostermeyer, H. (1974). Milchwissenschaft 29, 517.Google Scholar