Hostname: page-component-cd9895bd7-jkksz Total loading time: 0 Render date: 2024-12-18T04:46:54.903Z Has data issue: false hasContentIssue false
  • English
  • Français

Milk trypsin-inhibitor capacity as an indicator of bovine mastitis – a novel principle which can be automated*

Published online by Cambridge University Press:  01 June 2009

Markus Sandholm ,
Tuula Honkanen-Buzalski  and
Reima Kangasniemi
Markus Sandholm
Affiliation:
Department of Pharmacology and Toxicology, College of Veterinary Medicine, Hämeentie 57, Helsinki 5, Finland
Tuula Honkanen-Buzalski
Affiliation:
Department of Pharmacology and Toxicology, College of Veterinary Medicine, Hämeentie 57, Helsinki 5, Finland
Reima Kangasniemi
Affiliation:
Department of Pharmacology and Toxicology, College of Veterinary Medicine, Hämeentie 57, Helsinki 5, Finland
Get access Rights & Permissions [Opens in a new window]

Summary

As mastitis is associated with leakage of small molecular weight plasma proteins, such as α1-antitrypsin, into milk, this antitrypsin can be used as an indicator of mastitis. A colorimetric procedure was developed for large scale monitoring of milk antitrypsin activity, using microtitration plates and the Multiskan system. The effect of stage of lactation and age of the cow on the antitrypsin concentration and its interrelationship with other mastitis indicators (bovine serum albumin (BSA), somatic cell count) was analysed by computer programs on 1029 cows. Milk antitrypsin activity was high after parturition owing to colostral inhibitors. After the first month of lactation the assay measures only blood-derived antitrypsin and is a good indicator for detecting an increased permeability between blood and milk due to mastitis. Increasing lactation number only slightly affected the antitrypsin and BSA concentrations whereas somatic cell content was markedly affected.

Type
Original Articles
Information
Journal of Dairy Research , Volume 51 , Issue 1 , February 1984 , pp. 1 - 9
Copyright
Copyright © Proprietors of Journal of Dairy Research 1984

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Fritz, H., Trautschold, I. & Werle, E. 1974 Protease inhibitors. In Methods of Enzymatic Analysis 2nd edn vol. II pp. 1064–1080 (Ed. Bergmeyer, H. U.). New York: Academic PressCrossRefGoogle Scholar
Giesecke, W. H. & Viljoen, M. H. 1974 The diagnosis of subclinical mastitis in lactating cows: a comparison of cytological methods and a monovalent radial immunodiffusion test. Onderstepoort Journal of Veterinary Research 41 51–74Google Scholar
Honkanen-Buzalski, T., Katila, T. & Sandholm, M. 1981 a Milk antitrypsin activity during clinical and experimental bovine mastitis. Acta Veterinaria Scandinavica 22 360–368CrossRefGoogle ScholarPubMed
Honkanen-Buzalski, T., Kangasniemi, R., Atroshi, F. & Sandholm, M. 1981 b Effect of lactation stage and number on milk albumin (BSA) and somatic cell count. Zentralblatt für Veterinärmedizin A 28 760–767CrossRefGoogle ScholarPubMed
Honkanen-Buzalski, T. & Sandholm, M. 1981 Trypsin-inhibitora in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents. Journal of Dairy Research 48 213–223CrossRefGoogle ScholarPubMed
Laskowski, M. & Kato, I. 1980 Protein inhibitors of proteinases. Annual Review of Biochemistry 49 593–626.CrossRefGoogle ScholarPubMed
Mancini, G., Carbonara, A. O. & Heremans, J. F. 1965 Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2 235–254CrossRefGoogle ScholarPubMed
Rodbard, D. & Munson, P. J. 1980 Radioimmunoassay data processing. In Manual of Clinical Immunology pp. 343–349 (Eds Rose, N. R. and Friedman, H.). Washington, D.C.: American Society of MicrobiologyGoogle Scholar
Sandholm, M. & Honkanen-Buzalski, T. 1979 Colostral trypsin-inhibitor capacity in different animal species. Acta Veterinaria Scandinavica 20 469–476CrossRefGoogle ScholarPubMed
Schalm, O. W., Carroll, E. J. & Jain, N. C. 1971 Bovine Mastitis 372 pp. Philadelphia: Lea & FebigerGoogle Scholar
Schmidt Madsen, P. 1975 Fluoro-opto-electronic cell counting of milk. Bulletin, International Dairy Federation Document no. 85 pp. 133–135Google Scholar
Searle, S. R. 1971 Linear Models 553 pp. New York: John Wiley & Sons Inc.Google Scholar