Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-05T09:31:05.483Z Has data issue: false hasContentIssue false
  • English
  • Français

Milk β-hydroxybutyrate concentration measured by Fourier-transform infrared and flow-injection analyses from samples taken at different times relative to milking

Published online by Cambridge University Press:  30 April 2019

Mélissa Duplessis ,
Débora E. Santschi ,
Sabrina Plante ,
Camille Bergeron ,
Daniel M. Lefebvre ,
Jean Durocher  and
Roger I. Cue
Mélissa Duplessis
Affiliation:
Valacta, Sainte-Anne-de-Bellevue, Québec, H9X 3R4, Canada
Débora E. Santschi*
Affiliation:
Valacta, Sainte-Anne-de-Bellevue, Québec, H9X 3R4, Canada
Sabrina Plante
Affiliation:
Département des sciences animales, Université Laval, Québec, Québec, G1V 0A6, Canada
Camille Bergeron
Affiliation:
Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, Québec, H9X 3V9, Canada
Daniel M. Lefebvre
Affiliation:
Valacta, Sainte-Anne-de-Bellevue, Québec, H9X 3R4, Canada
Jean Durocher
Affiliation:
Valacta, Sainte-Anne-de-Bellevue, Québec, H9X 3R4, Canada
Roger I. Cue
Affiliation:
Department of Animal Science, McGill University, Sainte-Anne-de-Bellevue, Québec, H9X 3V9, Canada
*
Author for correspondence: Débora E. Santschi, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Analysis of milk BHB concentration by Fourier-transform infrared (FTIR) spectrometry more frequently than regular milk testing could help dairy producers in decision making, particularly if it would be possible to use small hand-stripped samples (hereinafter simply called samples) taken between dairy herd improvement (DHI) test-samples analysed using DHI algorithms. The aim of this Research Communication was to evaluate milk BHB concentration and the prevalence of elevated milk BHB concentration analysed by FTIR spectrometry compared with flow-injection analysis (SKALAR) from samples taken at different times relative to the milking. A total of 293 early-lactation cows in 44 commercial dairy herds were involved in the study. Herds were visited once during the morning milking when a routine DHI test-sample was obtained using in-line milk samplers. Additional milk samples were taken by hand stripping as follows: (1) Just before connecting the milking machine; (2) immediately after removing the milking machine; (3) 3 h after milking and (4) 6 h after milking. Milk samples were analysed for BHB concentration by FTIR and SKALAR, the latter being the reference method. Milk BHB concentration from samples taken before milking was different between FTIR and SKALAR whereas no difference was noted for other sampling times, although milk BHB concentration rose as time after milking increased. Except for DHI test-samples for which prevalence was not different between analysis methods, prevalence of elevated milk BHB concentration (≥0.15 mmol/l) was greater for FTIR analysis. However, no difference in prevalence was observed between SKALAR and FTIR when using a threshold of ≥0.20 mmol/l. In summary, hand-stripped milk samples taken any time after removing the milking machine until 6 h after the milking can be recommended for FTIR analysis of elevated milk BHB concentration prevalence provided a threshold of 0.20 mmol/l is used.

Keywords

Cowdairy herd improvementFourier-transform infraredβ-hydroxybutyratemilk
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 208 - 210
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

*

Present address: Agriculture et Agroalimentaire Canada, Centre de recherche et développement de Sherbrooke, Sherbrooke, Québec, J1M 0C8, Canada.

References

Canadian Council on Animal Care 2009 Guide to the Care and Use of Experimental Animals, 2nd Edn. Ottawa, Ontario, Canada: Canadian Council on Animal Care.Google Scholar
Denis-Robichaud, J, Dubuc, J, Lefebvre, D & DesCôteaux, L 2014 Accuracy of milk ketone bodies from flow-injection analysis for the diagnosis of hyperketonemia in dairy cows. Journal of Dairy Science 97 3364–3370.Google Scholar
de Roos, APW, van den Bijgaart, HJCM, Hørlyk, J & de Jong, G 2007 Screening for subclinical ketosis in dairy cattle by Fourier transform infrared spectrometry. Journal of Dairy Science 90 1761–1766.Google Scholar
Duplessis, M, Cue, RI, Santschi, DE, Lefebvre, DM & Girard, CL 2018 Short communication: relationships among plasma and milk vitamin B12, plasma free fatty acids, and blood β-hydroxybutyrate concentrations in early lactation dairy cows. Journal of Dairy Science 101 8559–8565.Google Scholar
Foss 2009 Foss Application Note 35. MilkoScan FT+ Ketosis Calibrations. Hillerød, Denmark: Foss.Google Scholar
Goff, JP & Horst, RL 1997 Physiological changes at parturition and their relationship to metabolic disorders. Journal of Dairy Science 80 1260–1268.Google Scholar
McArt, JAA, Nydam, DV & Oetzel, GR 2012 Epidemiology of subclinical ketosis in early lactation dairy cattle. Journal of Dairy Science 95 5056–5066.Google Scholar
Raboisson, D, Mounié, M & Maigné, E 2014 Diseases, reproductive performance, and changes in milk production associated with subclinical ketosis in dairy cows: a meta-analysis and review. Journal of Dairy Science 97 7547–7563.Google Scholar
Santschi, DE, Lacroix, R, Durocher, J, Duplessis, M, Moore, RK & Lefebvre, DM 2016 Prevalence of elevated milk β-hydroxybutyrate concentrations in Holstein cows measured by Fourier-transform infrared analysis in Dairy Herd Improvement milk samples and association with milk yield and components. Journal of Dairy Science 99 9263–9270.Google Scholar
SAS Institute 2012 User's Guide: Statistics. Version 9.4. Cary, NC, USA: SAS Institute.Google Scholar
Supplementary material: PDF

Duplessis et al. supplementary material

Duplessis et al. supplementary material 1

Download Duplessis et al. supplementary material(PDF)
PDF 326 KB