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Milk fatty acid variability: effect of some candidate genes involved in lipid synthesis

Published online by Cambridge University Press:  11 March 2013

Cinzia Marchitelli ,
Giovanna Contarini ,
Giovanna De Matteis ,
Alessandra Crisà ,
Lorraine Pariset ,
Maria Carmela Scatà ,
Gennaro Catillo ,
Francesco Napolitano  and
Bianca Moioli
Cinzia Marchitelli
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Giovanna Contarini
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Lombardo 19, 26900 Lodi, Italy
Giovanna De Matteis
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Alessandra Crisà
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Lorraine Pariset
Affiliation:
University of Tuscia, via de Lellis, 01100 Viterbo, Italy
Maria Carmela Scatà
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Gennaro Catillo
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Francesco Napolitano
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
Bianca Moioli*
Affiliation:
Consiglio per la ricerca e la sperimentazione in agricoltura, via Salaria 31, 00015 Monterotondo, Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

In this work, the genetic variation of milk FA was investigated in three different bovine breeds, the Jersey, the Piedmontese and the Valdostana, and at different lactation stages. All animals were genotyped for 21 Single Nucleotide Polymorphisms located within nine candidate genes involved in lipid synthesis: diacylglycerol acyltransferase 1 and 2 (DGAT1, 2); stearoyl-CoA desaturase (SCD); growth hormone receptor (GHR); fatty acid synthase (FASN); acyl-CoA dehydrogenase (ACAD); fatty acid binding protein (FABP4); lipoprotein lipase (LPL); and leptin gene (LEP). The highest milk-fat Jersey breed also showed the highest content of saturated FA. Throughout lactation, the breeds showed a similar variation in the FA, with a decrease in the short-chain, this was accompanied by a general increase in the long chain FA at the end of lactation. The increase in long chain saturated FA was particularly evident in the case of the Jersey. The effect of SCD gene on the C14 desaturation index was confirmed; the DGAT1 gene was polymorphic only in the Jersey breed, but its effect was confirmed only on milk fat content; three further potential candidate genes were identified: first, the FABP4 gene, which was found to influence medium and long chain FA in all the breeds, but not the desaturation indices; second, the FASN gene, which was found to influence the amount of PUFA in the Piedmontese and the Valdostana, and third, the LPL gene, which was found to affect fat content in the Piedmontese.

Keywords

Milk fatty acidsSingle Nucleotide PolymorphismCandidate genes
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 2 , May 2013 , pp. 165 - 173
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013

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