Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-16T01:20:25.984Z Has data issue: false hasContentIssue false

Mild protein hydrolysation of lactose-free milk further reduces milk-related gastrointestinal symptoms

Published online by Cambridge University Press:  01 April 2016

Anu Turpeinen*
Affiliation:
Valio Ltd, R&D, P.O. Box 30, 00039 Valio, Finland
Hanna Kautiainen
Affiliation:
Valio Ltd, R&D, P.O. Box 30, 00039 Valio, Finland
Marja-Leena Tikkanen
Affiliation:
Valio Ltd, R&D, P.O. Box 30, 00039 Valio, Finland
Timo Sibakov
Affiliation:
Valio Ltd, R&D, P.O. Box 30, 00039 Valio, Finland
Olli Tossavainen
Affiliation:
Valio Ltd, R&D, P.O. Box 30, 00039 Valio, Finland
Eveliina Myllyluoma
Affiliation:
Valio Ltd, R&D, P.O. Box 30, 00039 Valio, Finland
*
*For correspondence; e-mail: [email protected]

Abstract

Gastrointestinal symptoms associated with milk are common. Besides lactose, milk proteins may cause symptoms in sensitive individuals. We have developed a method for mild enzymatic hydrolysation of milk proteins and studied the effects of hydrolysed milk on gastrointestinal symptoms in adults with a self-diagnosed sensitive stomach. In a double blind, randomised placebo-controlled study, 97 subjects consumed protein-hydrolysed lactose-free milk or commercially available lactose-free milk for 10 d. Frequency of gastrointestinal symptoms during the study period was reported and a symptom score was calculated. Rumbling and flatulence decreased significantly in the hydrolysed milk group (P < 0·05). Also, the total symptom score was lower in subjects who consumed hydrolysed milk (P < 0·05). No difference between groups was seen in abdominal pain (P = 0·47) or bloating (P = 0·076). The results suggest that mild enzymatic protein hydrolysation may decrease gastrointestinal symptoms in adults with a sensitive stomach.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anthoni, S 2009 Cow's milk related gastrointestinal symptoms in adults. Doctoral Thesis, University of Helsinki, Helsinki University PrintGoogle Scholar
Becker, A, Hempel, G, Grecksch, G & Matthies, H 1990 Effects of beta-casomorphin derivatives on gastrointestinal transit in mice. Biomedica Biochimica Acta 49 12031207Google Scholar
Bengtsson, U, Knutson, TW, Knutson, L, Dannaeus, A, Hallgren, R & Ahlstedt, S 1997 Eosinophil cationic protein and histamine after intestinal challenge in patients with cow`s milk intolerance. Journal of Allergy and Clinical Immunology 100 216221Google Scholar
Claustre, J, Toumi, F, Trompette, A, Jourdan, G, Guignard, H, Chayvialle, JA & Plaisancie, P 2002 Effects of peptides derived from dietary proteins on mucus secretion in rat jejunum. American Journal of Physiology- Gastrointestinal and Liver Physiology 283 521528Google Scholar
Crittenden, RG & Bennett, LE 2005 Cow's milk allergy – a complex disorder. Journal of American College of Nutrition 24 582S591SGoogle Scholar
Daniel, H, Vohwinkel, M & Rehner, G 1990 Effect of casein and {beta}-casomorphins on gastrointestinal motility in rats. Journal of Nutrition 120 252257Google Scholar
De Noni, I 2008 Release of β -casomorphins 5 and 7 during simulated gastro-intestinal digestion of bovine β-casein variants and milk-based infant formulas. Food Chemistry 110 897903Google Scholar
Ho, S, Woodford, K, Kukuljan, S & Pal, S 2014 Comparative effects of A1 versus A2 beta-casein on gastrointestinal measures: a blinded randomized cross-over pilot study. European Journal of Clinical Nutrition 68 9941000Google Scholar
Høst, A 2002 Frequency of cow's milk allergy in childhood. Annals of Allergy, Asthma and Immunology 89 3337Google Scholar
Koopman, R, Crombach, N, Gijsen, AP, Walrand, S, Fauquant, J, Kies, AK, Lemosquet, S, Saris, WHM, Boirie, Y & van Loon, LJC 2009 Ingestion of a protein hydrolysate is accompanied by an accelerated in vivo digestion and absorption rate when compared with its intact protein. American Journal of Clinical Nutrition 90 106115CrossRefGoogle ScholarPubMed
Korbes, HJ, van der Kemp, HJ, Frankhuisen, R & de Jong, J 1994 Quantitative Determination of Skimmed Milk Powder in Animal Feedstuffs by FPLC. RIKILT Method. Netherlands: State Institute for Quality Control of Agricultural ProductsGoogle Scholar
Laemmli, UK 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680685Google Scholar
Martini, MC & Savaiano, DA 1988 Reduced intolerance symptoms from lactose consumed during meal. American Journal of Clinical Nutrition 47 5760Google Scholar
Matsubara, H, Hagihara, B, Nakai, M, Komaki, T, Yonetani, T & Okunuki, K 1958 Crystalline bacterial proteinase II. General properties of crystalline proteinase of Bacillus subtilis N’. Journal of Biochemistry 45 251258Google Scholar
Miralles, B, Rothbauer, V, Manso, MA, Amigo, L, Krause, I & Ramos, M 2001 Improved method for the simultaneous determination of whey proteins, caseins and para-κ-casein in milk and dairy products by capillary electrophoresis. Journal of Chromatography A 915 225230CrossRefGoogle ScholarPubMed
Pal, S, Woodford, K, Kukuljan, S & Ho, S 2015 Milk intolerance, beta-casein and lactose. Nutrients 7 72857297Google Scholar
Patel, SM, Stason, WB, Legedza, A, Ock, SM, Kaptchuk, TJ, Conboy, L, Canenguez, K, Park, JK, Kelly, E, Jacobson, E, Kerr, CE & Lembo, AJ 2005 The placebo effect in irritable bowel syndrome trials: a meta-analysis. Neurogastroenterology and Motility 17 332340CrossRefGoogle ScholarPubMed
Pelto, L, Impivaara, O, Salminen, S, Poussa, T, Seppänen, R & Lilius, EM 1999 Milk hypersensitivity in young adults. European Journal of Clinical Nutrition 53 620624CrossRefGoogle ScholarPubMed
Pelto, L, Salminen, S, Lilius, EM, Nuutila, J & Isolauri, E 1998 Milk hypersensitivity–key to poorly defined gastrointestinal symptoms in adults. Allergy 53 307310CrossRefGoogle ScholarPubMed
Rice, BH, Quann, EE & Miller, GD 2013 Meeting and exceeding dairy recommendations: effects of dairy consumption on nutrient intakes and risk of chronic disease. Nutrition Reviews 71 209223Google Scholar
Ulanova, M, Torebring, M, Porcelli, SA, Bengtsson, U, Magnusson, J, Magnusson, O, Lin, XP, Hanson, LA & Telemo, E 2000 Expression of CD1d in the duodenum of patients with cow's milk hypersensitivity. Scandanavian Journal of Immunology 52 609617Google Scholar
Vesa, TH, Marteau, PR, Briet, FB, Boutron-Ruault, MC & Rambaud, JC 1997 Raising milk energy content retards gastric emptying in lactose-intolerant humans with little effect on lactose digestion. Journal of Nutrition 127 23162320Google Scholar