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A method for the quantitative determination of individual free fatty acids in milk by ion exchange resin adsorption and gas-liquid chromatography

Published online by Cambridge University Press:  01 June 2009

Eric C. Needs ,
Graeme D. Ford ,
A. Jane Owen ,
Brian Tuckley  and
Malcolm Anderson
Graeme D. Ford
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
A. Jane Owen
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
Brian Tuckley
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
Malcolm Anderson
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading RG2 9 AT, UK
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Summary

A quantitative method for rapid routine analysis of individual free fatty acids (FFA) in milk was developed. Lipid was extracted from milk in ether and FFA were recovered by shaking the extract with anion exchange resin Amberlyst 26. The resin-bound FFA were methylated directly and the individual acids quantified, using internal standards, by gas-liquid chromatography. The properties of the resin were measured. The validity of the method was established by extraction of FFA mixtures and milk. Individual acids were, on average, found to be within 6% of the actual concentration present in the mixture. An average coefficient of variation of 4·3% was achieved for the major individual fatty acids on repeated extraction of a single milk sample.

Type
Original Articles
Information
Journal of Dairy Research , Volume 50 , Issue 3 , August 1983 , pp. 321 - 329
Copyright
Copyright © Proprietors of Journal of Dairy Research 1983

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References

REFERENCES

Bills, D. D., Khatri, L. L. & Day, E. A. 1963 Method for the determination of free fatty acids in milk. Journal of Dairy Science 46 1342–1347CrossRefGoogle Scholar
Carroll, K. K. 1962 Studies on the mechanisms by which erucic acid affects cholesterol metabolism. Distribution of erucic acid in adrenal and plasma lipids. Canadian Journal of Biochemistry 40 1115–1122Google ScholarPubMed
Castberg, H. B. & Solberg, P. 1974 The lipoprotein lipase and the lipolysis in bovine milk. Meieriposten 63 961–975Google Scholar
Christie, W. W. 1978 The composition, structure and function of lipids in the tissues of ruminant animals. Progress in Lipid Research 17 111–205CrossRefGoogle ScholarPubMed
Connolly, J. F., Murphy, J. J., O'Connor, C. B. & Headon, D. R. 1979 Relationship between lipolysed flavour and free fatty acid levels in milk and butter. Irish Journal of Food Science and Technology 3 79–92Google Scholar
Edwards–Webb, J. D. 1975 Determination of fatty acids in digesta: an improved method for the free fatty acids from C4:0 to C18:3. Journal of the Science of Food and Agriculture 26 1943–1948CrossRefGoogle Scholar
Iyer, M., Richardson, J., Amundson, C. & Boudreau, A. 1967 Improved technique for analysis of free fatty acids in butter oil and Provolone cheese. Journal of Dairy Science 50 285–291CrossRefGoogle Scholar
Hemingway, E. B., Smith, G. H. & Rook, J. A. F. 1970 The pattern of release of free fatty acids from milk fat under the action of intrinsic and added lipases. Journal of Dairy Research 37 83–96CrossRefGoogle Scholar
Hornstein, I., Alford, J. A., Elliott, L. E. & Crowe, P. F. 1960 Determination of free fatty acids in fat. Analytical Chemistry 32 540–542CrossRefGoogle Scholar
Kintner, J. A. & Day, E. A. 1965 Major free fatty acids in milk. Journal of Dairy Science 48 1575–1581.CrossRefGoogle Scholar
McCarthy, R. D. & Duthie, A. M. 1962 A rapid quantitative method for the separation of free fatty acids from other lipids. Journal of Lipid Research 3 117–119CrossRefGoogle Scholar
Randolph, M. E. & Erwin, R. E. 1974 Influence of mastitis on properties of milk. X. Fatty acid composition. Journal of Dairy Science 57 865–868CrossRefGoogle ScholarPubMed
Salih, A. M. A., Anderson, M. & Tuckley, B. 1977 The determination of short-and long-chain free fatty acids in milk. Journal of Dairy Research 44 601–605CrossRefGoogle Scholar
Scanlan, R. A., Sather, L. A. & Day, E. A. 1965 Contribution of free fatty acids to the flavour of rancid milk. Journal of Dairy Science 48 1582–1584CrossRefGoogle Scholar
Storry, J. E., Brumby, P. E., Tuckley, B., Welch, V. A., Stead, D. & Fulford, R. J. 1980 Effect of feeding protected lipid to dairy cows in early lactation on the composition of blood lipoproteins and secretion of fatty acids in milk. Journal of Agricultural Science 94 503–516CrossRefGoogle Scholar
Woo, A. H. & Lindsay, R. C. 1980 a Development and characterization of an improved silicic acid–KOH arrestant column for routine quantitative isolation of free fatty acids. Journal of the American Oil Chemists Society 57 414–416.CrossRefGoogle Scholar
Woo, A. H. & Lindsay, R. C. 1980 b Method for the routine quantitative gas chromatographic analysis of major free fatty acids in butter and cream. Journal of Dairy Science 63 1058–1064CrossRefGoogle ScholarPubMed