Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-27T04:47:27.208Z Has data issue: false hasContentIssue false
  • English
  • Français

Intraruminal administration of Megasphaera elsdenii modulated rumen fermentation profile in mid-lactation dairy cows

Published online by Cambridge University Press:  13 October 2011

Qendrim Zebeli ,
Sarah J Terrill ,
Alberto Mazzolari ,
Suzanna M Dunn ,
Wen Z Yang  and
Burim N Ametaj
Qendrim Zebeli*
Affiliation:
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5
Sarah J Terrill
Affiliation:
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5
Alberto Mazzolari
Affiliation:
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5
Suzanna M Dunn
Affiliation:
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5
Wen Z Yang
Affiliation:
Research Centre, Agriculture and Agri-Food Canada, Lethbridge, Alberta, CanadaT1J 4B1
Burim N Ametaj*
Affiliation:
Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada, T6G 2P5
*
†For correspondence; e-mail: [email protected]
†For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

This study evaluated the effects of intraruminal administration of Megasphaera elsdenii on ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day of M. elsdenii ATCC 25940 (MEGA), containing 108 cfu/ml of bacteria, dissolved in 35 ml of saline (0·15 m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate that M. elsdenii has the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.

Keywords

Megasphaera elsdeniidairy cowsshort-chain fatty acidsplasma metabolites
Type
Research Article
Information
Journal of Dairy Research , Volume 79 , Issue 1 , February 2012 , pp. 16 - 25
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aikman, PC, Henning, PH, Horn, CH & Humphries, DJ 2009 Use of Megasphaera elsdenii NCIMB 41125 as a probiotic for early-lactation dairy cows: Effects on rumen pH and fermentation patterns. Journal of Dairy Science 92 (E Suppl. 2) 289Google Scholar
Allison, MJ, Bryant, MP & Doetsch, RN 1962 Studies on the metabolic function of branched-chain volatile fatty acids, growth factors for Ruminococci. I. Incorporation of isovalerate into leucine. Journal of Bacteriology 83 523–532CrossRefGoogle ScholarPubMed
Aleman, MM, Stein, DR, Allen, DT, Perry, E, Lehloenya, KV, Rehberger, TG, Mertz, KJ, Jones, DA & Spicer, LJ 2007 Effects of feeding two levels of Propionibacteria to dairy cows on plasma hormones and metabolites. Journal of Dairy Research 74 146–153CrossRefGoogle ScholarPubMed
Andries, JI, Buysse, FX, De Brabander, DL & Cottyn, BG 1987 Isoacids in ruminant nutrition: Their role in ruminal and intermediary metabolism and possible influences on performances—a review. Animal Feed Science and Technology 18 169–180CrossRefGoogle Scholar
Barcenilla, A, Pryde, SE, Martin, JC, Duncan, SH, Stewart, CS, Henderson, C & Flint, HJ 2000 Phylogenetic relationships of butyrate-producing bacteria from the human gut. Applied and Environmental Microbiology 66 1654–1661CrossRefGoogle ScholarPubMed
Bauman, DE & Davis, CL 1974 Biosynthesis of milk fat. In: Lactation: a Comprehensive Treatise Volume 2 (Eds. Larson, BL & Smith, VR), pp. 31–75. New York, USA: Academic PressGoogle Scholar
Bauman, DE & Griinari, JM 2003 Nutritional regulation of milk fat synthesis. Annual Reviews of Nutrition 23 203–227CrossRefGoogle ScholarPubMed
Beauchemin, KA, Yang, WZ, Morgavi, DP, Ghorbani, GR, Kautz, W & Leedle, JA 2003 Effects of bacterial direct-fed microbials and yeast on site and extent of digestion, blood chemistry, and subclinical ruminal acidosis in feedlot cattle. Journal of Animal Science 81 1628–1640CrossRefGoogle ScholarPubMed
Bergman, EN & Wolff, JE 1971 Metabolism of volatile fatty acids by liver and portal-drained viscera in sheep. American Journal of Physiology 221 586–592CrossRefGoogle ScholarPubMed
Counotte, GH, Prins, RA, Janssen, RH & Debie, MJ 1981 Role of Megasphaera elsdenii in the fermentation of DL-[2-c]lactate in the rumen of dairy cattle. Applied and Environmental Microbiology 42 649–655CrossRefGoogle ScholarPubMed
Enemark, JM 2008 The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): a review. Veterinary Journal 176 32–43CrossRefGoogle ScholarPubMed
Ghorbani, GR, Morgavi, DP, Beauchemin, KA & Leedle, JA 2002 Effects of bacterial direct-fed microbials on ruminal fermentation, blood variables, and the microbial populations of feedlot cattle. Journal of Animal Science 80 1977–1985CrossRefGoogle ScholarPubMed
Henning, PH, Horn, CH, Leeuw, KJ, Meissner, HH & Hagg, FM 2010a Effect of ruminal administration of the lactate-utilizing strain Megasphaera elsdenii (ME) NCIMB 41125 on abrupt or gradual transition from forage to concentrate diets. Animal Feed Science and Technology 157 20–29CrossRefGoogle Scholar
Henning, PH, Horn, CH, Steyn, DG, Meissner, HH & Hagg, FM 2010b The potential of Megasphaera elsdenii isolates to control ruminal acidosis. Animal Feed Science and Technology 157 13–19CrossRefGoogle Scholar
Iqbal, S, Zebeli, Q, Mazzolari, A, Bertoni, G, Dunn, SM, Yang, WZ & Ametaj, BN 2009 Feeding barley grain steeped in lactic acid modulates rumen fermentation patterns and increases milk fat content in dairy cows. Journal of Dairy Science 92 6023–6032CrossRefGoogle ScholarPubMed
Iqbal, S, Zebeli, Q, Mazzolari, A, Dunn, SM & Ametaj, BN 2010 Feeding rolled barley grain steeped in lactic acid modulated energy status and innate immunity in dairy cows. Journal of Dairy Science 93 5147–5156CrossRefGoogle ScholarPubMed
Leeuw, KJ, Siebrits, FK, Henning, PH & Meissner, HH 2009 Effect of Megasphaera elsdenii NCIMB 41125 drenching on health and performance of steers fed high and low roughage diets in the feedlot. South African Journal of Animal Science 39 337–348Google Scholar
Liepa, GU, Beitz, DC & Linder, JR 1978 Cholesterol synthesis in ruminating and nonruminating goats. Journal of Nutrition 108 535–543CrossRefGoogle ScholarPubMed
Marounek, M, Fliegrova, K & Bartos, S 1989 Metabolism and some characteristics of ruminal strains of Megasphaera elsdenii. Applied and Environmental Microbiology 55 1570–1573CrossRefGoogle ScholarPubMed
Mentschel, J, Leiser, R, Mulling, C, Pfarrer, C & Claus, R 2001 Butyric acid stimulates rumen mucosa development in the calf mainly by a reduction of apoptosis. Archiv fur Tierernarhrung 55 85–102CrossRefGoogle ScholarPubMed
Miura, H, Horiguchi, M, & Matsumoto, T 1980 Nutritional inderdependence among rumen bacteria, Bacteroides amylophilus, Megasphaera elsdenii and Ruminococus albus. Applied and Environmental Microbiology 40 294–300CrossRefGoogle Scholar
Mouriño, F, Akkarawongsa, R & Weimer, PJ 2001 Initial pH as a determinant of cellulose digestion rate by mixed ruminal microorganisms in vitro. Journal of Dairy Science 84 848–859CrossRefGoogle ScholarPubMed
Nelson, DL & Cox, MM 2005 Lehninger Principles Biochemistry 4th Revised Edn. New York NY, USA: WHFreeman and CompanyGoogle Scholar
Nocek, JE & Kautz, WP 2006 Direct-fed microbial supplementation on ruminal digestion, health, and performance of pre- and postpartum dairy cattle. Journal of Dairy Science 89 260–266CrossRefGoogle ScholarPubMed
Nocek, JE, Kautz, WP, Leedle, JAZ & Block, E 2003 Direct-fed microbial supplementation on the performance of dairy cattle during the transition period. Journal of Dairy Science 86 331–335CrossRefGoogle ScholarPubMed
NRC 2001 Nutrient Requirements of Dairy Cattle. 7th Revised Edn. Washington DC, USA: National Academy of SciencesGoogle Scholar
Russell, JB & Baldwin, RL 1978 Substrate preferences in rumen bacteria: evidence of catabolite regulatory mechanisms. Applied and Environmental Microbiology 36 319–329CrossRefGoogle ScholarPubMed
Rychlik, JL, LaVera, R & Russell, JB 2002 Amino acid deamination by ruminal Megasphaera elsdenii strains. Current Microbiology 45 340–345CrossRefGoogle ScholarPubMed
SAS Institute, 2003 SAS User's Guide: Statistics. Release 9.13. Sas Inst. Inc., Cary NC, USAGoogle Scholar
Satter, LD & Esdale, WJ 1968 In vitro lactate metabolism by ruminal ingesta. Journal of Applied Microbiology 16 680–688CrossRefGoogle ScholarPubMed
Van Soest, PJ, Robertson, JB & Lewis, BA 1991 Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74 3583–3597CrossRefGoogle ScholarPubMed
Zebeli, Q, Dijkstra, J, Tafaj, M, Steingass, H, Ametaj, BN & Drochner, W 2008 Modeling the adequacy of dietary fiber in dairy cows based on the responses of ruminal pH and milk fat production to composition of the diet. Journal of Dairy Science 91 2046–2066CrossRefGoogle ScholarPubMed
Zebeli, Q & Ametaj, BN 2009 Relationships between rumen lipopolysaccharide and mediators of inflammatory response with milk fat production and efficiency in dairy cows. Journal of Dairy Science 92 3800–3809CrossRefGoogle ScholarPubMed
Zebeli, Q, Mansmann, D, Ametaj, BN, Steingass, H & Drochner, W 2010 A model to optimise the requirements of lactating dairy cows for physically effective neutral detergent fibre. Archives of Animal Nutrition 64 265–278CrossRefGoogle Scholar