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Interleukin-6 in quarter milk as a further prediction marker for bovine subclinical mastitis

Published online by Cambridge University Press:  07 December 2010

Yoko Sakemi ,
Yutaka Tamura  and
Katsuro Hagiwara
Yoko Sakemi
Affiliation:
Food Microbiology and Food Safety, School of Veterinary Medicine, Rakuno Gakuen University, 582, Midorimachi, Bunkyodai, Ebetsu-shi, Hokkaido 069-8501, Japan
Yutaka Tamura
Affiliation:
Food Microbiology and Food Safety, School of Veterinary Medicine, Rakuno Gakuen University, 582, Midorimachi, Bunkyodai, Ebetsu-shi, Hokkaido 069-8501, Japan
Katsuro Hagiwara*
Affiliation:
Veterinary Virology, School of Veterinary Medicine, Rakuno Gakuen University, 582, Midorimachi, Bunkyodai, Ebetsu-shi, Hokkaido 069-8501, Japan
*
*For correspondence; e-mail: [email protected]
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Abstract

Subclinical mastitis (SM) is a major concern in the dairy industry because it causes economic losses and can lead to clinical mastitis. The mechanisms of the onset and progression of SM are not fully understood, and a new procedure for the detection and appropriate prediction of SM leading to clinical mastitis is necessary for dairy cow management. Inflammatory cytokines such as interleukin (IL)-6 are known to be present in inflamed mammary glands at the onset of mastitis, and IL-6 concentrations correlate with the levels of inflammation. In this study, the detection of IL-6 was examined for the evaluation for the future prediction of SM in 77 quarter milk samples from 20 cows. IL-6 concentrations in quarter milk were measured by sandwich ELISA, and the data were compared with milk somatic cell count (SCC) levels to diagnose SM. Average IL-6 concentration was significantly higher in SM quarter milk (207·0±441·6 pg/ml) than in healthy control quarter milk (12·6±33·4 pg/ml, P<0·01). Results of the cross-tabulation table show that SM prediction accuracy based on IL-6 concentration was almost equal or superior to SM prediction accuracy based on SCC. The detection of IL-6 in milk indicated SM earlier than did the detection of elevated SCC. Thus, the detection of IL-6 in milk could be a future prediction marker for SM.

Keywords

Interleukinsubclinical mastitisquarter milk
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 1 , February 2011 , pp. 118 - 121
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Ball, HJ & Greer, D 1991 N-acetyl-beta-D-glucosaminidase test for screening milk samples for subclinical mastitis. Veterinary Record 129 507–509Google ScholarPubMed
Droogmans, L, Cludts, I, Cleuter, Y, Kettmann, R & Burny, A 1992 Nucleotide sequence of bovine interleukin-6 cDNA. DNA Sequence 2 411–413CrossRefGoogle ScholarPubMed
Hagiwara, K, Domi, M & Ando, J 2008 Bovine colostral CD8-positive cells are potent IFN-gamma-producing cells. Veterinary Immunology and Immunopathology 124 93–98CrossRefGoogle ScholarPubMed
Hagiwara, K, Yamanaka, H, Hisaeda, K, Taharaguchi, S, Kirisawa, R & Iwai, H 2001 Concentrations of IL-6 in serum and whey from healthy and mastitic cows. Veterinary Research and Communication 25 99–108CrossRefGoogle ScholarPubMed
LeBlanc, SJ, Lissemore, KD, Kelton, DF, Duffield, TF & Leslie, KE 2006 Major advances in disease prevention in dairy cattle. Journal of Dairy Science 89 1267–1279CrossRefGoogle ScholarPubMed
Middleton, JR, Hardin, D, Steevens, B, Randle, R & Tyler, JW 2004 Use of somatic cell counts and California mastitis test results from individual quarter milk samples to detect subclinical intramammary infection in dairy cattle from a herd with a high bulk tank somatic cell count. Journal of American Veterinary Medical Association 224 419–423CrossRefGoogle ScholarPubMed
Musser, JM, Anderson, KL, Caballero, M, Amaya, D & Maroto-Puga, J 1998 Evaluation of a hand-held electrical conductivity meter for detection of subclinical mastitis in cattle. American Journal of Veterinary Research 59 1087–1091CrossRefGoogle ScholarPubMed
Nakajima, Y, Mikami, O, Yoshioka, M, Motoi, Y, Ito, T, Ishikawa, Y, Fuse, M, Nakano, K & Yasukawa, K 1997 Elevated levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) activities in the sera and milk of cows with naturally occurring coliform mastitis. Research in Veterinary Science 62 297–298CrossRefGoogle ScholarPubMed
Okada, H, Ohtsuka, H, Kon Nai, S, Kirisawa, R, Yokomizo, Y, Yoshino, T & Rosol, TJ 1999 Effects of lipopolysaccharide on production of interleukin-1 and interleukin-6 by bovine mammary epithelial cells in vitro. Journal of Veterinary Medical Science 61 33–35CrossRefGoogle ScholarPubMed
Pyorala, S 2003 Indicators of inflammation in the diagnosis of mastitis. Veterinary Research 34 565–578CrossRefGoogle Scholar
Riollet, C, Rainard, P & Poutrel, B 2000 Cells and cytokines in inflammatory secretions of bovine mammary gland. Advances in Experimental Medicine and Biology 480 247–258CrossRefGoogle ScholarPubMed
Ruegg, PL 2003 Investigation of mastitis problems on farms. Veterinary Clinics of North America Food Animal Practice 19 47–73CrossRefGoogle ScholarPubMed
Tyler, JW & Cullor, JS 2002 Mammary gland health and disorders. In: Large Animal Internal Medicine 3rd Edition, (Ed. Smith, BP) pp. 1019–1038. St. Louis MO, USA: Mosby Inc.Google Scholar
Urech, E, Puhan, Z & Schallibaum, M 1999 Changes in milk protein fraction as affected by subclinical mastitis. Journal of Dairy Science 82 2402–2411CrossRefGoogle ScholarPubMed