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The influence of milk composition on pH and calcium activity measured in situ during heat treatment of reconstituted skim milk

Published online by Cambridge University Press:  03 March 2010

Jayani Chandrapala*
Affiliation:
School of Chemistry, Monash University, VIC 3800, Australia CSIRO Division of Food and Nutritional Sciences, 671 Sneydes Road, WerribeeVIC 3030, Australia
Ian McKinnon
Affiliation:
School of Chemistry, Monash University, VIC 3800, Australia
Mary Ann Augustin
Affiliation:
CSIRO Division of Food and Nutritional Sciences, 671 Sneydes Road, WerribeeVIC 3030, Australia
Punsandani Udabage
Affiliation:
CSIRO Division of Food and Nutritional Sciences, 671 Sneydes Road, WerribeeVIC 3030, Australia
*
*For correspondence; e-mail: [email protected]

Abstract

The pH and calcium activity of reconstituted skim milk solutions (9–21% w/w milk solids non-fat) on heating and after cooling were studied as a function of milk pH prior to heating (pH 6·2–7·2 at 25°C) and added calcium complexing agents (phosphate or EDTA). The pH decreased as the temperature was raised from 25 to 90°C and the magnitude of the pH decrease was greater with increase in initial pH at 25°C before heating or milk concentration. The pH decrease on heating from 25 to 90°C in skim milk solutions with added calcium complexing agents was lower than that of milk without the addition of these salts. The calcium activity decreased on heating from 25 to 60°C. The magnitude of the change decreased with increase in initial pH at 25°C before heating and milk concentration. The decrease in calcium activity on heating from 25 to 60°C for skim milk solutions with added calcium complexing agents was lower than that of milk solutions without the addition of calcium complexing agents. The changes in pH and calcium activity on heating milk were largely reversible after cooling the milk. The results suggested that the pH and calcium activity at high temperatures are a function of the milk composition. Knowledge of the initial pH prior to heating alone is not sufficient for predicting the changes that occur during heating.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Aoki, T, Yamada, N & Kako, Y 1990 Relation between colloidal calcium phosphate cross-linkage and release of β-casein from bovine casein micelles on cooling. Agricultural Biological Chemistry 54(9) 22872292Google Scholar
Augustin, M & Clarke, PT 1990 Effects of added salts on the heat stability of recombined concentrated milk. Journal of Dairy Research 57 213226Google Scholar
Augustin, M & Clarke, PT 1991 Calcium ion activities of cooled and aged reconstituted and recombined milks. Journal of Dairy Research 58 219229CrossRefGoogle Scholar
Augustin, M & Udabage, P 2007 Chapter 1 Influence of processing on functionality of milk and dairy proteins. Advances in food and nutrition research 53 138Google Scholar
Castro, IM, Corzo, N & Olano, A 1986 Modifications and interactions of lactose with mineral components of milk during heating process. Food Chemistry 21 211221Google Scholar
Chaplin, LC & Lyster, RLJ 1988 Effect of temperature on the pH of skim milk. Journal of Dairy Research 55 277280CrossRefGoogle Scholar
CRC handbook of CHEMISTRY & PHYSICS 55th Edition. 19741975. CRC press, Cleveland, Ohio, 1974Google Scholar
Dalgleish, DG & Law, AJR 1989 pH induced dissociation of bovine casein micelles 2. Mineral solubilisation and its relation to casein release. Journal of Dairy Research 56 727735Google Scholar
De La Fuente, MA 1998 Changes in the mineral balance of milk submitted to technological treatments. Trends in Food Science and Technology 9 281288Google Scholar
De La Fuente, MA, Olano, A & Juarez, M 2002 Mineral balance in milk heated using microwave energy. Journal of Agricultural and Food Chemistry 50 22742277Google Scholar
Gaucher, I, Piot, M, Beacher, E & Gaucheron, F 2007 Physico-chemical characterization of phosphate added skim milk. International Dairy Journal 17 13751383CrossRefGoogle Scholar
Geertz, JP, Bekhof, JJ & Scherjon, JW 1983 Determination of calcium ion activities in milk with an ion selective electrode. Netherlands Milk Dairy Journal 37 197211Google Scholar
Goddard, SJ & Augustin, MA 1995 Formation of acid-heat induced skim milk gels in the pH range 5- 5·7: effect of the addition of salt and Ca chelating agents. Journal of Dairy Research 62 491500CrossRefGoogle Scholar
Hardy, EE, Muir, DD, Sweetsur, AWM & West, IG 1984 Changes of calcium phosphate partition and heat stability during manufacture of sterilized concentrated milk. Journal of Dairy Science 67 16661673Google Scholar
Holt, C 2004 An equilibrium thermodynamic model of the sequestration of calcium phosphate by casein micelles and its application to the calculation of the partition of salts in milk. European Biophysics Journal 33 421434CrossRefGoogle Scholar
International Dairy Federation 1964 Dried Milk: Determination of the water content. Brussels: IDF (FIL-IDF Standard no. 26)Google Scholar
International Dairy Federation 1987 Dried Milk: Determination of Sodium and Potassium Contents (Flame Photometric Method) Brussels: IDF (FIL-IDF Standard no. 119A)Google Scholar
International Dairy Federation 1990 Milk determination of total Phosphorus content Spectrometric Method. Brussels: IDF (FIL-IDF Standard no. 42B)Google Scholar
International Dairy Federation 1993 Milk: Determination of Nitrogen Content. Brussels: IDF (FIL-IDF Standard no. 20B)Google Scholar
Le Ray, C, Maubois, JL, Gaucheron, F, Brule, G, Pronnier, P & Garnier, F 1998 Heat stability of reconstituted casein micelle dispersions: Changes induced by salt addition. Lait 78 375390CrossRefGoogle Scholar
Lin, M, Lewis, MJ & Grandison, AS 2006 Measurements of ionic calcium in milk. International Journal of Dairy Technology 59 192199CrossRefGoogle Scholar
Ma, Y & Barbano, DM 2003 Milk pH as a function of CO2 concentration, temperature and pressure in a heat exchanger. Journal of Dairy Science 86 38223830Google Scholar
McKinnon, IR & Chandrapala, J 2006 Calcium binding in milk and cheese –interactions between aggregates. The Australian Journal of Dairy Technology 61(2) 154156Google Scholar
Morr, CV 1985 Functionality of heated milk proteins in dairy and related foods. Journal of Dairy Science 68(10) 2773–81Google Scholar
Nieuwenhuijse, j, Timmermans, W & Walstra, P 1988 Calcium and phosphate partitions during the manufacture of sterilized concentrated milk and their relations to the heat stability. Netherlands Milk Dairy Journal 42 387421Google Scholar
O'Connell, JE & Fox, PF 2001 Effect of β-Lactoglobulin and precipitation of calcium phosphate on the thermal coagulation of milk. Journal of Dairy Research 68 8194Google Scholar
Oldfield, DJ, Singh, H & Taylor, MW 2005 Kinetics of heat-induced whey protein denaturation and aggregation in skim milks with adjusted whey protein concentration. Journal of Dairy Research 72 369378Google Scholar
Parris, N & Baginski, MA 1991 A rapid method for the determination of whey protein denaturation. Journal of Dairy Science 74 5864Google Scholar
Pouliot, Y, Boulet, M & Paquin, P 1989a Observations on the heat induced salt balance changes in milk: Effect of heating time between 4 and 90°C. Journal of Dairy Research 56 185192Google Scholar
Pouliot, Y, Boulet, M & Paquin, P 1989b Observations on the heat induced salt balance changes in milk: Reversibility on cooling. Journal of Dairy Research 56 193199Google Scholar
Singh, H 2004 Heat stability of milk. International Journal of Dairy Technology 57 111119CrossRefGoogle Scholar
Standards Association of Australia 1988 Methods of chemical and physical testing of the dairying industry. General methods and principles – Determination of ash (SAA AS2300.1.5)Google Scholar
Standards Association of Australia 1991 Methods of chemical and physical testing for the dairying industry. General methods and principles – Determination of Nitrogen -Reference Kjeldahl Method (AS2300.1.2.1)Google Scholar
Standards Association of Australia 1994 Methods of chemical and physical testing for the dairying industry. Dried milk and dried milk products – Determination of lactose – titrimetric method (AS2300.4.10)Google Scholar
Udabage, P 1999 The composition and the renneting behaviour of skim milk. PhD thesis – Department of Chemistry, Monash University, AustraliaGoogle Scholar
Udabage, P, McKinnon, IR & Augustin, M 2000 Mineral equillibria in milk: Effects of added salts and calcium chelating agents. Journal of Dairy Research 67 361370CrossRefGoogle Scholar
Van Boekel, MAJS, Nieuwenhuijse, JA & Walstra, P 1989 The heat coagulation of milk. 1. Mechanisms. Netherlands Milk Dairy Journal 43 97–127Google Scholar
Van Hooydonk, ACM, Hagedoorn, HG & Boerrigter, IJ 1986 pH-induced physico-chemical changes of casein micelles in milk and their effect on renneting. 1. Effect of acidification on physico-chemical properties. Netherlands Milk Dairy Journal 40 281296Google Scholar
Xiong, YL, Dawson, KA & Wan, L 1993 Thermal aggregation of β- Lactoglobulin: Effect of pH, ionic environment and thiol reagent. Journal of Dairy Science 76 7077CrossRefGoogle Scholar
Zhang, G & Aoki, T 1996 Behaviour of calcium and phosphate in bovine casein micelles. International Dairy Journal 6 769780Google Scholar
Zhang, G, Foegeding, EA & Hardin, CC 2004 Effect of sulfated polysaccharides on heat induced structural changes in β-Lactoglobulin. Journal of Agricultural and Food Chemistry 52 39753981CrossRefGoogle ScholarPubMed