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Indirect assay of milk clotting activity of Mucor miehei proteinase (Fromase) in combined rennets

Published online by Cambridge University Press:  01 June 2009

Eva Beránková ,
Pavel Rauch ,
Jan Káš ,
Vladimír Hušek  and
Eduard Paluska
Eva Beránková
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Pavel Rauch
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Jan Káš
Affiliation:
Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28 Prague 6, Czechoslovakia
Vladimír Hušek
Affiliation:
Dairy Research Institute, 198 00 Prague 9, Czechoslovakia
Eduard Paluska
Affiliation:
Institute of Hematology and Blood Transfusion, 128 20 Prague 2, Czechoslovakia
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Summary

Milk clotting activity of Mucor miehei proteinase (commercial name Fromase) was determined in combined rennets as the difference between the total activity of combined rennet and the residual activity after inhibition of Fromase by the purified immunoglobulin fraction of a Fromase antiserum. The proposed method is simple, rapid, and makes possible the use of any arbitrary method for assaying milk clotting activity. The application of the immunoglobulin fraction, instead of the whole antiserum, is necessary owing to the presence of nonspecific proteinase inhibitors in blood serum.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 3 , August 1987 , pp. 407 - 412
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

REFERENCES

Arnon, R. 1973 In The Antigens vol. 1, p. 87 (Ed. Sela, M.). New York, London: Academic PressCrossRefGoogle Scholar
Berridge, N. J. 1955 Purification and assay of rennin. Methods in Enzymology 2 69–77CrossRefGoogle Scholar
Davjssant, J. 1977 Immunochemical approach to questions related to α- and β-amylases in barley and wheat. In Enzymes in Food and Beverage Processing pp. 80–99 (Eds Ory, R. L. and Angelo, A. J. St). Washington, DC: American Chemical Society (ACS Symposium Series no. 47)CrossRefGoogle Scholar
Daussant, J. & Skakoun, A. 1974 Combination of absorption technique and α-amylase activity determination in the same gel medium. Journal of Immunological Methods 4 127–133CrossRefGoogle ScholarPubMed
Green, M. L. 1977 Review of the progress of Dairy Science: milk coagulants. Journal of Dairy Research 44 159–188CrossRefGoogle Scholar
Harboe, M. K. 1981 Analysis of milk-clotting enzymes in commercial rennets. Netherlands Milk and Dairy Journal 35 367–399Google Scholar
Harboe, N. & Inoild, A. 1973 Immunization, isolation of immunoglobulins, estimation of antibody titre. In Quantitative Immunoelectrophoresis pp. 1–167 (Ed. Axelsen, N. H.) Oslo: Universitetsforlaget (Scandinavian Journal of Immunology 2 (Supplement I))Google Scholar
Matheson, A. R. 1981 The immunochemical determination of chymosin activity in cheese. New Zealand Journal of Dairy Science and Technology 16 33–41Google Scholar
McPhie, P. 1976 A turbidimetric milk-clotting assay for pepsin. Analytical Biochemistry 73 258–261CrossRefGoogle ScholarPubMed
Mulvihill, D. M. & Fox, P. F. 1977 Selective denaturation of milk coagulants in 5 M-urea. Journal of Dairy Research 44 319–324CrossRefGoogle Scholar
Ouchterlony, Ö. 1949 Antigen-antibody reaction in gels. Acta Pathologica et Microbiologic Scandinavica 26 507–515CrossRefGoogle ScholarPubMed
Rothe, G. A. L., Axelsen, N. H., Johnk, P. & Foltmann, B. 1976 Immunochemical, chromatographic, and milk-clotting activity measurements for quantification of milk-clotting enzymes in bovine rennets. Journal of Dairy Research 43 85–95CrossRefGoogle ScholarPubMed