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Identification of bovine mastitis pathogens using MALDI-TOF mass spectrometry in Brazil

Published online by Cambridge University Press:  10 August 2021

Thais Cristina de Assis Oliveira ,
Maria Aparecida Vasconcelos Paiva Brito ,
Marcia Giambiagi-de Marval ,
Nívea Maria Vicentini  and
Carla Christine Lange
Thais Cristina de Assis Oliveira
Affiliation:
Graduate Program in Science and Technology of Milk and Derivatives, Federal University of Juiz de Fora, Juiz de Fora, Brazil
Maria Aparecida Vasconcelos Paiva Brito
Affiliation:
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil
Marcia Giambiagi-de Marval
Affiliation:
Department of Medical Microbiology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
Nívea Maria Vicentini
Affiliation:
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil
Carla Christine Lange*
Affiliation:
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil
*
Author for correspondence: Carla Christine Lange, Email: [email protected]
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Abstract

In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.

Keywords

Bovine intramammary infectiongram-negative bacteriamass spectrometrystaphylococcistreptococci
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 302 - 306
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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References

Alnakip, MEA, Rhouma, NR, Abd-Elfatah, EM, Quintela-Baluja, M, Böhme, K, Fernandez-No, I, Bayoumi, MA, Abdelhafez, MM, Taboada-Rodríguez, A, Calo-Mata, P and Barros-Velázquez, J (2020) Discrimination of major and minor streptococci incriminated in bovine mastitis by MALDI-TOF MS fingerprinting and 16S rRNA gene sequencing. Research in Veterinary Science 132, 426–438.CrossRefGoogle ScholarPubMed
Cameron, M, Saab, M, Heider, L, McClure, JT, Rodrigues-Lecompte, JC and Sanchez, J (2016) Antimicrobial susceptibility patterns of environmental streptococci recovered from bovine milk samples in the maritime provinces of Canada. Frontiers in Veterinary Science 3, 1–14.CrossRefGoogle ScholarPubMed
Cameron, M, Barkema, HW, De Buck, J, De Vliegher, S, Chaffer, M, Lewis, J and Keefe, GP (2017) Identification of bovine-associated coagulase-negative staphylococci by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a direct transfer protocol. Journal of Dairy Science 100, 2137–2147.CrossRefGoogle ScholarPubMed
Cherkaoui, A, Emonet, S, Fernandes, J, Schorderet, D and Scherezel, J (2011) Evaluation of matrix-assisted laser desorption-time of flight mass spectrometry for rapid identification of beta-hemolytic streptococci. Journal of Clinical Microbiology 49, 3004–3005.CrossRefGoogle Scholar
Condas, LAZ, De Buck, J, Nobrega, DB, Carson, DA, Naushad, S, De Vliegher, S, Zadoks, RN, Middleton, JR, Dufour, S, Kastelic, JP and Barkema, HW (2017) Prevalence of non-aureus Staphylococcus species causing intramammary infections in Canadian dairy herds. Journal of Dairy Science 100, 5592–5612.CrossRefGoogle ScholarPubMed
Lange, CC, Brito, MAVP, Reis, DRL, Machado, MA, Guimarães, AS, Azevedo, ALS, Sales, EB, Alvim, MCT, Silva, FS and Meurer, IR (2015) Species-level identification of staphylococci isolated from bovine mastitis in Brazil using partial 16S rRNA sequencing. Veterinary Microbiology 176, 382–388.CrossRefGoogle ScholarPubMed
NMC – National Mastitis Council (2004) Microbiological Procedures for the Diagnosis of Bovine Udder Infection and Determination of Milk Quality, 4 Edn. Verona: National Mastitis Council.Google Scholar
Nonnemann, B, Lyhs, U, Svennesen, L, Kristensen, KA, Klaas, IC and Pedersen, K (2019) Bovine mastitis bacteria resolved by MALDI-TOF mass spectrometry. Journal of Dairy Science 102, 2515–2524.CrossRefGoogle ScholarPubMed
Raemy, A, Meylan, M, Casati, S, Gaia, V, Berchtold, B, Boss, R and Wyder, A (2013) Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections. Acta Veterinaria Scandinavica 55, 53–61.CrossRefGoogle ScholarPubMed
Rodrigues, NMB, Bronzato, GF, Santiago, GS, Botelho, LAB, Moreira, BM, Coelho, IS, Souza, MMS and Coelho, SMO (2016) The Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) identification vs. biochemical tests: a study with enterobacteria from a dairy cattle environment. Brazilian Journal of Microbiology 48, 132–138.CrossRefGoogle Scholar
Schmidt, T, Kock, MM and Ehlers, MM (2018) Identification and characterization of Staphylococcus devriesei isolates from bovine intramammary infections in KwaZulu-Natal, South Africa. BMC Veterinary Research 14, 1–10.CrossRefGoogle ScholarPubMed
Singhal, N, Kumar, M, Kanaujia, PK and Virdi, JS (2015) MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Frontiers in Microbiology, 6, 791.CrossRefGoogle ScholarPubMed
Wilson, DJ, Middleton, JR, Adkins, PRF and Goodell, GM (2019) Test agreement among biochemical methods, matrix-assisted laser desorption ionization-time of flight mass spectrometry, and 16S rRNA sequencing for identification of microorganisms isolated from bovine milk. Journal of Clinical Microbiology 57, 1–8.CrossRefGoogle ScholarPubMed
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