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Identification of Bacillus strains isolated from milk and cream with classical and nucleic acid hybridization methods

Published online by Cambridge University Press:  01 June 2009

Ralf Tatzel ,
Wolfgang Ludwig ,
Karl Heinz Schleifer  and
Peter R. Wallnöfer
Ralf Tatzel
Affiliation:
Bayerische Landesanstalt für Ernährung, 80638 München, Deutschland
Wolfgang Ludwig
Affiliation:
Lehrstuhl für Mikrobiologie, Technische Universität München, 80290 München, Deutschland
Karl Heinz Schleifer
Affiliation:
Lehrstuhl für Mikrobiologie, Technische Universität München, 80290 München, Deutschland
Peter R. Wallnöfer
Affiliation:
Bayerische Landesanstalt für Ernährung, 80638 München, Deutschland
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Summary

A total of 529 bacterial strains have been isolated from milk and cream sampled at different sites in a dairy production plant under conditions selective for aerobic sporeforming bacteria. Identification with classical methods based on morphological, physiological and biochemical criteria showed Bacillus licheniformis to be the most frequently occurring Bacillus sp. The investigation also revealed 62 unidentified strains. Classical identification methods were time consuming (3–7d), lacked specificity and —because of their dependence on phenotypic gene expression—sometimes produced ambiguous results. Consequently, a colony hybridization method developed for the identification of B. licheniformis strains and using non-radioactive labelled 23S rRNA targeted oligonucleotide probes was also used. Identification of B. licheniformis with borth classical and hybridization methods revealed diverging identification results for 70 strains.

Type
Original articles
Information
Journal of Dairy Research , Volume 61 , Issue 4 , November 1994 , pp. 529 - 535
Copyright
Copyright © Proprietors of Journal of Dairy Research 1994

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References

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