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Identification and verification of differentially expressed genes in yak mammary tissue during the lactation cycle

Published online by Cambridge University Press:  19 March 2020

Mao Yuan ,
Wei Xia ,
Xiaolei Zhang ,
Yongtao Liu  and
Mingfeng Jiang
Mao Yuan
Affiliation:
The Research Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, State Ethnic Affairs Commission and Ministry of Education, Chengdu, China
Wei Xia
Affiliation:
The Research Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, China College of Life Science and Technology, Southwest Minzu University, Chengdu, China
Xiaolei Zhang
Affiliation:
College of Life Science and Technology, Southwest Minzu University, Chengdu, China
Yongtao Liu
Affiliation:
College of Life Science and Technology, Southwest Minzu University, Chengdu, China
Mingfeng Jiang*
Affiliation:
The Research Institute of Qinghai-Tibet Plateau, Southwest Minzu University, Chengdu, China College of Life Science and Technology, Southwest Minzu University, Chengdu, China Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, State Ethnic Affairs Commission and Ministry of Education, Chengdu, China
*
Author for correspondence: MingFeng Jiang, Email: [email protected]
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Abstract

Yaks (Bos grunniens) live primarily in the Qinghai-Tibetan plateau (altitude: 2000–5000 m). Their milk presents unusual characteristics, containing large amounts of solids including fat and protein, and it is, therefore, important to understand the genetic makeup of the yak. To identify potentially critical genes playing a role in yak mammary tissue from colostrum to mature milk phase of lactogenesis, the early lactation (colostrum) stage (ELS; day 1 after parturition) and mature lactation (milk) stage (MLS; day 15) were chosen for comparison. An ELS-specific cDNA library was established by suppression subtractive hybridization and 25 expressed sequence tags at ELS were identified by sequencing and alignment. To further confirm our results the expression levels of 21 genes during the lactation cycle were measured using quantitative real-time RT-PCR (qRT-PCR). The qRT-PCR results confirmed 9 significantly up-regulated genes at ELS vs. MLS in yak mammary tissue, in which the l-amino acid oxidase 1 (LAO1) and collagen, type I, alpha I (COL1A1) were the most significantly up-regulated. During the lactation cycle, the highest expression of some milk fat genes (i.e., XDH and FABP3) in yak mammary tissue appears earlier than that in dairy cow. Our data also indicate MYC potentially playing a central role through putative regulation of COL1A1, CD44, SPARC, FASN and GPAM.

Keywords

Differentially expressed genesearly lactation stagemammary tissueYak
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 158 - 165
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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Footnotes

*

Mao Yuan and Wei Xia contributed equally to this work.

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