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Hypoglycaemic effect of galactooligosaccharides in alloxan-induced diabetic rats

Published online by Cambridge University Press:  10 November 2014

Vikas Sangwan ,
Sudhir K Tomar ,
Babar Ali ,
Ram R B Singh  and
Ashish K Singh
Vikas Sangwan
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
Sudhir K Tomar*
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
Babar Ali
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
Ram R B Singh
Affiliation:
Dairy Technology Division, National Dairy Research Institute, Karnal 132001, India
Ashish K Singh
Affiliation:
Dairy Technology Division, National Dairy Research Institute, Karnal 132001, India
*
*For correspondence; e-mail: [email protected]
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Abstract

This study was conducted to assess the effect of prebiotic galactooligosaccharides (GOS) on alloxan-induced diabetes in male Sprague-Dawley (SD) rats. Diabetes was induced by administration of alloxan (100 mg/kg) and rats were divided in 4 groups: normal control group (NCG), prebiotic control group (PCG), diabetic control group (DCG) and diabetic prebiotic group (DPG). While PCG and DPG were fed with GOS supplemented (10% w/w) diet, NCG and DCG were administered with basal diet. Rats were sacrificed after 42 d for collection of blood and liver. Faecal samples were collected at the interval of 7 d throughout the study for measurement of lactobacilli and coliform count. Feeding of GOS decreased or delayed the severity of diabetes by amelioration of diabetes associated markers including fasting blood glucose, haemoglobin, glycosylated haemoglobin triglycerides, total cholesterol, low density lipoproteins, creatinine and urea. GOS was also found to improve the levels of antioxidative enzymes (superoxide dismutase, catalase and glutathione peroxidase) in liver and blood. Improvement in lactobacilli count along with a concomitant decrease in coliform count was observed in GOS fed groups.

Keywords

Galactooligosaccharidesdiabetesprebioticshyperglycaemiaoxidative stress
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 1 , February 2015 , pp. 70 - 77
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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