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The folate-binding protein in milk

Published online by Cambridge University Press:  01 June 2009

J. E. Ford ,
D. N. Salter  and
K. J. Scott
J. E. Ford
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
D. N. Salter
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
K. J. Scott
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
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Summary

The folate in cow's milk was strongly and specifically bound to a minor whey protein (FP), forming a complex of primary M of about 38000, but exhibiting concentration-dependent reversible aggregation. The binding protein was present in excess, and the milk had the capacity to bind about 50 μg added folic acid/l. An enriched concentrate of FP was prepared by ammonium sulphate fractionation—FP was precipitated at between 50 and 60% saturation—and further purified by chromatography in DEAE-cellulose and filtration in Sephadex gel G150. Its identity as a distinct minor whey protein was confirmed by comparative starch gel electrophoresis at various pH values.

Some properties of the protein are described, and its physiological significance discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 36 , Issue 3 , October 1969 , pp. 435 - 448
Copyright
Copyright © Proprietors of Journal of Dairy Research 1969

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