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Focusing on casein gene cluster and protein profile in Garganica goat milk

Published online by Cambridge University Press:  05 January 2009

Marzia Albenzio ,
Antonella Santillo ,
Francesca d'Angelo  and
Agostino Sevi
Marzia Albenzio*
Affiliation:
Dipartimento PRIME. Università di Foggia, Italy Istituto per la Ricerca e le Applicazioni Biotecnologie per la Sicurezza e la Valorizzazione dei Prodotti Tipici e di Qualità. Università di Foggia, Italy
Antonella Santillo
Affiliation:
Dipartimento PRIME. Università di Foggia, Italy
Francesca d'Angelo
Affiliation:
Dipartimento PRIME. Università di Foggia, Italy
Agostino Sevi
Affiliation:
Dipartimento PRIME. Università di Foggia, Italy Istituto per la Ricerca e le Applicazioni Biotecnologie per la Sicurezza e la Valorizzazione dei Prodotti Tipici e di Qualità. Università di Foggia, Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

A survey was carried out in eight goat dairy farms, a total of 71 individual Garganica goat milk samples were collected for genomic DNA extraction. Casein alleles and haplotype frequencies of Garganica population were estimated. Individual milks were also analysed for chemical composition, rheological properties, and protein profile. The strong A* allele of CSN1S1 was predominant in the population investigated, the weak allele F of CSN1S1 showed a relatively high frequency and the null alleles N and 01 were first observed in this breed. At CSN1S2 locus the strong A* allele was the most frequent, followed by the F allele and the null allele. The strong A* allele was predominant at CSN2 locus, and relatively high incidence of null allele 0 was observed. CSN3 locus was monomorphic for B* allele. The exact test of sample differentiation based on haplotype frequencies discriminate the farms into two groups characterized by the highest frequency of strong (S-CSN1S1) or weak (W-CSN1S1) alleles at CSN1S1. Protein and casein contents were higher in the group characterized by strong allele than in the group with weak allele at CSN1S1. The 2D electrophoresis technique was performed to screen goat casein variability at the protein level and to evaluate global casein genotype (αs1, αs2, β and κ-CN). Gels displayed the protein profile associated with casein genotype, and demonstrated differences in the protein expression deriving from interactions between loci. The variability of goat casein loci in Garganica goat breed could be exploited to differentiate the population on the basis of milk utilization and could represent a strategy to preserve the genotype of this autochthonous breed.

Keywords

goatcasein genotypecasein polymorphismcheesemaking propertiesallergenicity
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 1 , February 2009 , pp. 83 - 89
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

Albenzio, M, Caroprese, M, Marino, R, Muscio, A, Santillo, A & Sevi, A 2006 Characteristics of Garganica goat milk and Cacioricotta cheese. Small Ruminant Research 66 35–44CrossRefGoogle Scholar
Amigo, L, Recio, I & Ramos, M 2000 Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk-a review. International Dairy Journal 10 135–149CrossRefGoogle Scholar
Bevilacqua, C, Martin, P, Candalh, C, Fauquant, J, Piot, M, Roucayroll, AM, Pilla, F & Heyman, M 2001 Goat's milk of defective alpha (s1) – casein genotype decreases intestinal and systemic sensitization to beta- lactoglobulin in guinea pigs. Journal of Dairy Research 68 217–227CrossRefGoogle ScholarPubMed
Chessa, S, Budelli, E, Chiatti, F, Cito, AM, Bolla, P & Caroli, A 2005. Predominance of β-casein (CSN2) C allele in goat breeds reared in Italy. Journal of Dairy Science 88 1878–1881CrossRefGoogle ScholarPubMed
Clark, S & Sherbon, JW 2000 Alpha s1-casein, milk composition and coagulation properties of goat milk. Small Ruminant Research 38 123–134CrossRefGoogle Scholar
Cosenza, G, Pauciullo, A, Gallo, D, Colimoro, L, D'Avino, A, Mancasi, A & Ramunno, L 2008 Genotyping at the CSN1S1 locus by PCR-RFLP and AS-PCR in a Napolitan goat population. Small Ruminant Research 74 84–90CrossRefGoogle Scholar
d'Angelo, F, Santillo, A, Sevi, A & Albenzio, M 2007 Technical Note: A simple salting-out method for DNA extraction from milk somatic cells: investigation into the goat CSN1S1 gene. Journal of Dairy Science 90 3550–3552CrossRefGoogle ScholarPubMed
El-Agamy, EI 2007 The challenge of cow milk protein allergy. Small Ruminant Research 68 64–72CrossRefGoogle Scholar
Excoffier, L, Laval, G & Schneider, S 2005 Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1 47–50Google Scholar
Feligini, M, Cubrik-Curik, V, Parma, P, Curik, I, Greppi, GF & Enne, G 2002 Polymorphism of k-Casein in Italian goat breeds: a new ACRS-PCR designed DNA test for discrimination of A and B alleles. Food Technology and Biotechnology 40 293–298Google Scholar
Feligini, M, Frati, S, Cubrik-Curik, V, Brambilla, A, Parma, P, Curik, I, Greppi, GF & Enne, G 2005 Caprine αs1-casein polymorfism: characterization of A, B, E and F variants by means of various biochemical and molecular techniques. Food Technology and Biotechnology 43 123–132Google Scholar
Grosclaude, F 1988 Genetic polymorphism of the main cow milk proteins. Relations with milk's quantity, composition and cheesemaking attitude. INRA Productions Animales 1 5–17CrossRefGoogle Scholar
Haenlein, G F W 2004 Goat milk in human nutrition. Small Ruminant Research 51 155–163CrossRefGoogle Scholar
Lagonigro, R, Pietrola, E, D'Andrea, M, Veltri, C & Pilla, F 2001 Molecular genetic characterization of the goat αs2-casein E allele. Animal Genetics 32 391–393CrossRefGoogle Scholar
Lara-Villoslada, F, Olivares, M & Xaus, J 2005 The balance between caseins and whey proteins in cow's milk determines its allergenicity. Journal of Dairy Science 88 1654–1660CrossRefGoogle ScholarPubMed
Leroux, C, Mazure, N & Martin, P 1992 Mutations away from splice site recognition sequences might cis-modulate alternative splicing of goat as1-casein transcripts. Structural organization of the relevant gene. Journal Biology Chemistry 267 6147–6157CrossRefGoogle Scholar
Martin, P, Szymanowska, M, Zwierzchowski, L & Leroux, C 2002 The impact of genetic polymorphisms on the protein composition of ruminant milks. Reproduction Nutrition Development 42 433–459CrossRefGoogle ScholarPubMed
Moioli, B, D'Andrea, M & Pilla, F 2007 Candidate genes affecting sheep and goat milk quality. Small Ruminant Research 68 179–192CrossRefGoogle Scholar
Natale, M, Bisson, C, Monti, G, Peltran, A, Perono Garoffo, L, Valentini, S, Fabris, C, Bertino, E, Coscia, A & Conti, A 2004 Cow's milk allergens identification by two-dimensional immunoblotting and mass spectrometry. Molecular Nutritional Food Research 48 363–369CrossRefGoogle ScholarPubMed
Pappalardo, M, Rando, A, Di Gregorio, P, Masina, P & Ramunno, L 1997 A MseI RFLP in the 5' DNA region of the goat β-casein gene. Animal Genetics 28 238–246Google Scholar
Park, YW, Juárez, M, Ramos, M & Haenlein, GFW 2007 Physico-chemical characteristics of goat and sheep milk. Small Ruminant Research 68 88–113CrossRefGoogle Scholar
Ramunno, L, Cosenza, G, Pappalardo, M, Longobardi, E, Gallo, D, Pastore, N, Di Gregorio, P & Rando, A 2001a Characterization of two new alleles at the goat CSN1S2 locus. Animal Genetics 32 264–268CrossRefGoogle ScholarPubMed
Ramunno, L, Longobardi, E, Pappalardo, M, Rando, A, Di Gregorio, P, Cosenza, G, Mariani, P, Pastore, N & Masina, P 2001b An allele associated with a non detectable amount of αs2-casein in goat milk. Animal Genetics 32 19–26CrossRefGoogle Scholar
Roncada, P, Gaviraghi, A, Liberatori, S, Canas, B, Bini, L & Greppi, GF 2002 Identification of caseins in goat milk. Proteomics 2 723–7263.0.CO;2-I>CrossRefGoogle ScholarPubMed
Sacchi, P, Chessa, S, Budelli, E, Bolla, P, Ceriotti, G, Soglia, D, Rasero, R, Cauvin, E & Caroli, A 2005 Casein haplotype structure in five Italian goat breeds. Journal of Dairy Science 88 1561–1568CrossRefGoogle ScholarPubMed
SAS 1999 SAS/STAT User's Guide (Version 8.1). Statistical Analysis System Inst, Cary, NCGoogle Scholar
Shapiro, SS & Wilk, M 1965 An analysis of variance test for normality. Biometrika 52 591–601CrossRefGoogle Scholar
Vassal, L, Delacroix-Buchet, A & Buillon, J 1994 Influence des variants AA, EE et FF de la caséine αs1 caprine sur le rendiment fromager et les caractéristiques sensorielles des fromages traditioanells: Premières observations. Le Lait 74 89–103CrossRefGoogle Scholar
Xie, X & Ott, J 1993 Testing linkage disequilibrium between a disease gene and marker loci. The American Journal of Human Genetics 53 1107Google Scholar
Yahyaoui, MH, Angiolillo, A, Pilla, F, Sanchez, A & Folch, JM 2003 Characterization and genotyping of the caprine kappa casein variants. Journal of Dairy Science 86 2715–2720CrossRefGoogle ScholarPubMed
Zullo, AC, Barone, AM, Chianese, L, Colatruglio, P, Occidente, M & Matassino, D 2005 Protein polymorphisms and coagulation properties of Cilentana goat milk. Small Ruminant Research 58 223–230CrossRefGoogle Scholar