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Flavour sulphides are produced from methionine by two different pathways by Geotrichum candidum

Published online by Cambridge University Press:  19 October 2000

YANN DEMARIGNY ,
CÉLINE BERGER ,
NATHALIE DESMASURES ,
MICHELINE GUEGUEN  and
HENRY E. SPINNLER
YANN DEMARIGNY
Affiliation:
Laboratoire de Microbiologie Alimentaire, Institut de Biochimie et de Biologie Appliqué, Esplanade de la Paix, Université de Caen Basse-Normandie, F-14000 Caen, France
CÉLINE BERGER
Affiliation:
Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National Agronomique Paris–Grignon, F-78850 Thiverval Grignon, France
NATHALIE DESMASURES
Affiliation:
Laboratoire de Microbiologie Alimentaire, Institut de Biochimie et de Biologie Appliqué, Esplanade de la Paix, Université de Caen Basse-Normandie, F-14000 Caen, France
MICHELINE GUEGUEN
Affiliation:
Laboratoire de Microbiologie Alimentaire, Institut de Biochimie et de Biologie Appliqué, Esplanade de la Paix, Université de Caen Basse-Normandie, F-14000 Caen, France
HENRY E. SPINNLER
Affiliation:
Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National Agronomique Paris–Grignon, F-78850 Thiverval Grignon, France
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Abstract

We have investigated the capacities of Geotrichum candidum strains to produce sulphides from methionine. This attribute is very important in cheese technology because of the flavouring potential of sulphur compounds. A spectrophotometric procedure using 5,5′-dithiobis(2-nitrobenzoic acid) to determine sulphides was tested on a collection of G. candidum strains, and confirmed by gas chromatography–mass spectrometry. The strains were distinguished on the basis of their ability to produce methanethiol. Gas chromatography–mass spectrometry also made it possible to identify other sulphides, such as dimethyl disulphide, dimethyl trisulphide and dimethyl sulphide. Using sonicated cells, the specific production of these four sulphides was studied in presence of L-[S-methyl-2H]methionine. Both methanethiol and dimethyl sulphide were produced from methionine, but two different pathways were used by G. candidum.

Type
Research Article
Information
Journal of Dairy Research , Volume 67 , Issue 3 , August 2000 , pp. 371 - 380
Copyright
Proprietors of Journal of Dairy Research 2000

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