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Enzymatic-fluorometric analyses for glutamine, glutamate and free amino groups in protein-free plasma and milk

Published online by Cambridge University Press:  02 March 2017

Torben Larsen  and
Carlos Fernández
Torben Larsen*
Affiliation:
Department of Animal Science, Aarhus University, Blichers Allé 20, PO Box 50, 8830 Tjele, Denmark
Carlos Fernández
Affiliation:
ACUMA Research Center, Polytechnic University of Valencia, 46022 Valencia, Spain
*
*For correspondence; e-mail: [email protected]
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Abstract

This Technical Research Communication describes new analytical methods for free, unbound glutamic acid and glutamine in protein-free blood plasma and milk and introduces the use of quantitation of free amino groups in the same matrices for descriptive and analytical purposes. The present enzymatic-fluorometric methods are easily performed within one working day, allowing for ‘high throughput’ assays of animal trials. These assays could support and enable further studies in lactation physiology with the objective of improved metabolic health.

Keywords

Milk metabolitesbiomarkersGlnGluOPA
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 1 , February 2017 , pp. 32 - 35
Copyright
Copyright © Proprietors of Journal of Dairy Research 2017 

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