Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-05T05:46:15.761Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of Staphylococcus aureus products on growth and function of bovine mammary myoepithelial cells in vitro

Published online by Cambridge University Press:  01 June 2009

Boris Zavizion ,
A. John Bramley  and
Ioannis Politis
Boris Zavizion
Affiliation:
Department of Animal and Food Sciences, University of Vermont, Burlington, VT 05405, USA
A. John Bramley
Affiliation:
Department of Animal and Food Sciences, University of Vermont, Burlington, VT 05405, USA
Ioannis Politis
Affiliation:
Department of Animal and Food Sciences, University of Vermont, Burlington, VT 05405, USA
Get access Rights & Permissions [Opens in a new window]

Summary

The effects of culture supernatants conditioned by the growth of Staphylococcus aureus M60 on in vitro growth and functional properties of bovine mammary myoepithelial cells were examined. Myoepithelial cell proliferation was reduced by Staph, aurexis M60 culture supernatants. Exposure of myoepithelial cells to culture supernatants of isogeneic mutants of Staph, aureus M60 that produced either α or β toxins reduced proliferation, but to a lesser extent than supernatants from the wild type strain. Thus, α and β toxins may play some role in affecting myoepithelial cell proliferation. Of the cells tested, 42% contracted following addition of oxytocin (10–7 M) in the culture medium. Treatment of myoepithelial cells for 15 min with Staph, aureus M60 supernatants, prior to addition of oxytocin in the culture medium, increased the number of cells that contracted to 92%. Exposure of cells for 3 h to the same supernatant, prior to addition of oxytocin in the culture medium, abolished oxytocin responsiveness, had no effect on immunolocalization of actin and vimentin, but affected the localization of α-actinin within myoepithelial cells. Treatment of myoepithelial cells for 3 h with a combination of purified staphylococcal proteinases XVI and XVII-B abolished oxytocin responsiveness and mimicked the effect of the Staph, aureus culture supernatant. We conclude that Staph, aureus M60 culture supernatant affected proliferation and functional properties of myoepithelial cells.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 4 , November 1995 , pp. 577 - 586
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bramley, A. J. & Dodd, F. H. 1984 Reviews of the progress of dairy science. Mastitis control—progress and prospects. Journal of Dairy Research 51 481–512CrossRefGoogle ScholarPubMed
Bramley, A. J., Patel, A. H., O'reilly, M., Foster, R. & Foster, T. J. 1989 Roles of alpha-toxin and betatoxin in virulence of Staphylococcus aureus for the mouse mammary gland. Infection and Immunity 57 2489–2494CrossRefGoogle ScholarPubMed
Darnell, J., Lodish, H. & Baltimore, D. 1990 Actin, myosin, and intermediate filaments: cell movements and cell shape.In Molecular Cell Biology, 2nd edn, pp. 859–902. New York: Scientific American BooksGoogle Scholar
Foster, T. J., O'Reilly, M., Patel, A. H. & Bramley, A. J. 1988 Genetic studies of Staphylococcus aureus virulence factors. Anionic van Leeuwenhoek Journal of Microbiology 54 475–182Google ScholarPubMed
Foster, T. J., O'Reilly, M., Patel, A. H., Nowlan, P. & Bramley, A. J. 1987 Genetic analysis of toxins and Protein A in the pathogenesis of Staphylococcus aureus infections: properties of an unexpressed alphatoxin locus from a clinical isolate. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Supplemente 17 343–349Google Scholar
Jonsson, P., Lindberg, M., Haraldsson, I. & Wadström, T. 1985 Virulence of Staphylococcus aureus in a mouse mastitis model: studies of alpha hemolysin, coagulase, and protein A as possible virulence determinants with protoplast fusion and gene cloning. Infection and Immunity 49 765–769CrossRefGoogle Scholar
Larson, B. L. 1985 Biosynthesis and cellular secretion of milk. In Lactation, 2nd edn, pp. 129–163 (Ed. Larson, B. L.). Ames, IA: Iowa State University PressGoogle Scholar
Matthews, K. R., Rejman, J. J., Turner, J. D. & Oliver, S. P. 1994 Proliferation of a bovine mammary epithelial cell line in the presence of bacterial virulence factors. Journal of Dairy Science 77 2959–2964CrossRefGoogle ScholarPubMed
Phonimdaeng, P., O'Reilly, M., Nowlan, P., Bramley, A. J. & Foster, T. J. 1990 The coagulase of Staphylococctis aureus 8325·4. Sequence analysis and virulence of site-specific coagulase-deficient mutants. Molecular Microbiology 4 393–404Google Scholar
SAS 1987 SAS User's Guide: Statistics. Cary, NC: SAS Institute.Google Scholar
Woolford, M. W. & Williamson, J. H. 1980 Milk production losses associated with clinical mastitis within identical-twin sets. Proceedings of the New Zealand Society of Animal Production 40 175–179Google Scholar
Zavizion, B., Politis, I. & Gorewit, R. C. 1992 a Bovine mammary myoepithelial cells. 1. Isolation, culture, and characterization. Journal of Dairy Science 75 3367–3380Google Scholar
Zavizion, B., Politis, I. & Gorewit, R. C. 1992 b Bovine mammary myoepithelial cells. 2. Interactions with epithelial cells in vitro. Journal of Dairy Science 75 3381–3393CrossRefGoogle ScholarPubMed
Zavizion, B., Bramley, A. J., Politis, I., Gilmore, J., Turner, J. D., Patel, A. H. & Foster, T. J. 1995 Effects of Staphylococcus aureus toxins on the growth of bovine mammary epithelial cells (MAC-T) in culture. Journal of Dairy Science 78 277–284CrossRefGoogle ScholarPubMed