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Effects of α-linolenic acid-enriched diets on gene expression of key inflammatory mediators in immune and milk cells obtained from Holstein dairy cows

Published online by Cambridge University Press:  12 February 2016

Pedram Rezamand ,
Brent P. Hatch ,
Kevin G. Carnahan  and
Mark A. McGuire
Pedram Rezamand*
Affiliation:
Department of Animal and Veterinary Science, University of Idaho, Moscow, ID 83844, USA
Brent P. Hatch
Affiliation:
Department of Animal and Veterinary Science, University of Idaho, Moscow, ID 83844, USA
Kevin G. Carnahan
Affiliation:
Department of Animal and Veterinary Science, University of Idaho, Moscow, ID 83844, USA
Mark A. McGuire
Affiliation:
Department of Animal and Veterinary Science, University of Idaho, Moscow, ID 83844, USA
*
*For correspondence; e-mail: [email protected]
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Abstract

Immune system and inflammatory responses are affected by α-linolenic acid (αLA: 18:3 ω-3). The objective of this study was to determine the effects of αLA-enriched rations on gene expression of systemic (blood) and local (mammary gland) inflammatory markers in Holstein dairy cattle. Further, the effect of dietary treatments was evaluated on the concentration of αLA in serum phospholipids. Camelina (Camelina sativa) meal (containing 24·2% αLA) was fed at 0, 3, 6, and 9% (dry matter basis) replacing canola meal (rich in 18:1 ω-9) to provide rations with incremental concentrations of αLA. Lactating primiparous Holstein cows (n = 18) were randomly assigned to a treatment sequence in a 4 × 4 Latin square design. Each period lasted 16 d and milk and blood samples were collected during the final 2 d of each period. Peripheral blood mononuclear cells (PBMC) and milk cells (MC) were harvested, and RNA extracted and converted to complementary DNA for quantitative real time PCR analysis. The effect of dietary treatments (αLA) on the relative abundance of pro- and anti-inflammatory genes in the PBMC and MC was tested by the MIXED procedure of SAS. Expression of pro-inflammatory tumour necrosis factor (TNF)-α in MC was linearly reduced (up to 40%) as dietary αLA increased. Expression of pro-inflammatory markers interleukin (IL)-1β, IL-8, and TNF-α was reduced (29, 20, and 27%, respectively) in PBMC isolated from cows fed 6% camelina meal ration as compared with cows fed 0% (control). Expression of IL-6 was, however, increased with inclusion of camelina meal. Greater dietary αLA linearly increased serum phospholipids αLA contents, and when fed up to 6% DM down-regulated expression of some of the local (milk) and systemic (blood) pro-inflammatory markers in vivo.

Keywords

Camelina mealgene expressioninflammatory markers
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 1 , February 2016 , pp. 20 - 27
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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References

Aiko, S, Yoshizumi, Y, Ishizuka, T, Horio, T, Sakano, T, Kumano, I, Kanai, N & Maehara, T 2008 Enteral immuno-enhanced diets with arginine are safe and beneficial for patients early after esophageal cancer surgery. Diseases of the Esophagus 21 619–627CrossRefGoogle ScholarPubMed
Aiko, S, Yoshizumi, Y, Tsuwano, S, Shimanouchi, M, Sugiura, Y & Maehara, T 2005 The effects of immediate enteral feeding with a formula containing high levels of omega-3 fatty acids in patients after surgery for esophageal cancer. Journal of Parenteral and Enteral Nutrition 29 141–147CrossRefGoogle ScholarPubMed
Alluwaimi, AM 2004 The cytokines of bovine mammary gland: prospects for diagnosis and therapy. Research in Veterinary Science 77 211–222CrossRefGoogle ScholarPubMed
Alluwaimi, AM, Farver, TB & Cullor, JS 2003 The transcriptional activity of IL-8 in healthy bovine mammary gland at mid and late stage of lactation period. Pakistan Journal of Biological Science 6 729–731CrossRefGoogle Scholar
Calder, PC 2002 Dietary modification of inflammation with lipids. Proceedings of the Nutrition Society 61 345–358CrossRefGoogle Scholar
Calder, PC 2006 Polyunsaturated fatty acids and inflammation. Prostaglandins Leukotrienes Essential Fatty Acids 75 197–202CrossRefGoogle ScholarPubMed
Christie, WW 1982 A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. Journal of Lipid Research 23 1072–1075CrossRefGoogle ScholarPubMed
Clark, RM, Ferris, AM, Fey, M, Brown, PB, Hundricser, KE & Jensen, RG 1982 Changes in the lipids of human milk from 2–16 weeks postpartum. Journal of Pediatric Gastroenterology and Nutrition 1 311–315CrossRefGoogle Scholar
Corl, CM, Gandy, JC & Sordillo, LM 2008 Platelet activating factor production and proinflammatory gene expression in endotoxin-challenged bovine mammary endothelial cells. Journal of Dairy Science 91 3067–3078CrossRefGoogle ScholarPubMed
Eigersma, A, Ellen, G, van der Horst, H, Boer, H, Dekker, PR & Tamminga, S 2004 Quick changes in milk fat composition from cows after transition from fresh grass to a silage diet. Animal Feed Science & Technology 117 13–27CrossRefGoogle Scholar
Feng, S, Salter, AM, Parr, T & Garnsworthy, PC 2007 Extraction and quantitative analysis of stearoyl-coenzyme A desaturase mRNA from dairy cow milk somatic cells. Journal of Dairy Science 90 4128–4136CrossRefGoogle ScholarPubMed
Field, C, Johnson, JIR & Schley, JD 2002 Nutrients and their role in host resistance to infection. Journal of Leukocyte Biology 71 16–32CrossRefGoogle ScholarPubMed
Griinari, JM, Dwyer, DA, McGuire, MA, Bauman, DE, Palmquist, DL & Nurmela, KVV 1998 Trans-octadecenoic acids and milk fat depression in lactating dairy cows. Journal of Dairy Science 81 1251–1261CrossRefGoogle ScholarPubMed
Hamilton, J & Comai, K 1988 Rapid separation of neutral lipids, free fatty acids and polar lipids using prepacked silica Sep-Pak columns. Lipids 23 1146–1149CrossRefGoogle ScholarPubMed
Hatch, BP 2013 Evaluation of Camelina Meal as a Protein and Omega-3 Source for Lactating Dairy Cattle. Master of Science Thesis. Moscow, ID, USA: Department of Animal and Veterinary Sciences, University of Idaho. http://www.worldcat.org/search?q=no%3A881857099.Google Scholar
Hurtaud, C & Peyraud, JL 2007 Effects of feeding camelina (seeds or meal) on milk fatty acid composition and butter spreadability. Journal of Dairy Science 90 5134–5145CrossRefGoogle ScholarPubMed
Kaplanski, G, Marin, V, Montero-Julian, F, Mantovani, A & Farnarier, C 2003 IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunology 24 25–29CrossRefGoogle ScholarPubMed
Lacetera, N, Scalia, D, Mashek, DG, Bernabucci, U & Grummer, RR 2007 Effects of intravenous triacylglycerol emulsions on lymphocyte responses to mitogens in fasted dairy cows undergoing intense lipomobilization. Journal of Dairy Research 74 323–328CrossRefGoogle ScholarPubMed
Lee, JY, Plakidas, A, Lee, WH, Heikkinen, A, Chanmugam, P, Bray, G & Hwang, DH 2003 Differential modulation of Toll-like receptors by fatty acids: preferential inhibition by n-3 polyunsaturated fatty acids. Journal of Lipid Research 44 479–486CrossRefGoogle ScholarPubMed
Lessard, M, Gagnon, N & Petit, HV 2003 Immune response of postpartum dairy cows fed flaxseed. Journal of Dairy Science 86 2647–2657CrossRefGoogle ScholarPubMed
Lopez-Garcia, E, Schulze, MB, Meigs, JB, Manson, JE, Rifai, N, Stampfer, MJ, Willett, WC & Hu, FB 2005 Consumption of trans-fatty acids is related to plasma biomarkers of inflammation and endothelial dysfunction. Journal of Nutrition 135 562–566CrossRefGoogle Scholar
Meydani, SN 1996 Effect of (n-3) polyunsaturated fatty acids on cytokine production and their biological function. Nutrition 12(1 Suppl.) S8–S12Google Scholar
Miles, EA & Calder, PC 1998 Modulation of immune function by dietary fatty acids. Procedures of the Nutrition Society 57 277–292CrossRefGoogle ScholarPubMed
Mosley, EE, Shafii, B, Moate, PJ & McGuire, MA 2006 cis-9, trans-11 conjugated linoleic acid Is synthesized directly from vaccenic acid in lactating dairy cattle. Journal of Nutrition 136 570–575CrossRefGoogle ScholarPubMed
Mosley, SA, Mosley, EE, Hatch, B, Szasz, JI, Corato, A, Zacharias, N, Howes, D & McGuire, MA 2007 Effect of varying levels of fatty acids from palm oil on feed intake and milk production in Holstein cows. Journal of Dairy Science 90 987–993CrossRefGoogle ScholarPubMed
Nickerson, SC, Owens, WE, Rejman, JJ & Oliver, SP 1993 Effects of interleukin-1 and interleukin-2 on mammary gland leukocyte populations and histology during the early nonlactating period. Zentralblatt fur Veterinarmedizin B 40 621–633Google ScholarPubMed
Park, YH, Fox, LK, Hamilton, MJ & Davis, WC 1993 Suppression of proliferative response of BoCD4+ T lymphocytes by activated BoCD8+ T lymphocytes in the mammary gland of cows with Staphylococcus aureus mastitis. Veterinary Immunology and Immunopathology 36 137–151CrossRefGoogle ScholarPubMed
Petit, H, Dewhurst, VRJ, Scollan, ND, Proulx, JG, Khalid, M, Haresign, W, Twagiramungu, H & Mann, GE 2002 Milk production and composition, ovarian function, and prostaglandin secretion of dairy cows fed omega-3 fats. Journal of Dairy Science 85 889–899CrossRefGoogle ScholarPubMed
Roy, A, Ferlay, A, Shingfield, KJ & Chilliard, Y 2006 Examination of the persistency of milk fatty acid composition responses to plant oils in cows given different basal diets, with particular emphasis on trans-C18:1 fatty acids and isomers of conjugated linoleic acid. Journal of Animal Science 82 479–492CrossRefGoogle Scholar
Shields, SL, Rezamand, P, Sevier, DL, Seo, KS, Price, W & McGuire, MA 2011 Effects of increased milking frequency during early postpartum on selected measures of mammary gland health, milk yield, and milk composition. Journal of Dairy Research 78 301–307CrossRefGoogle Scholar
Skeaff, CM, Hodson, L & McKenzie, JE 2006 Dietary-induced changes in fatty acid composition of human plasma, platelet, and erythrocyte lipids follow a similar time course. Journal of Nutrition 136 565–569CrossRefGoogle ScholarPubMed
von der Thüsen, JH, Kuiper, J, van Berkel, TJ & Biessen, EA 2003 Interleukins in atherosclerosis: molecular pathways and therapeutic potential. Pharmacology Review 55 133–166CrossRefGoogle ScholarPubMed
Wanten, GJ & Calder, PC 2007 Immune modulation by parenteral lipid emulsions. American Journal of Clinical Nutrition 85 1171–1184CrossRefGoogle ScholarPubMed
Watts, JS, Rezamand, P, Sevier, DL, Price, W & McGuire, MA 2013 Short-term ffects of dietary trans fatty acids compared with saturated fatty acids on selected measures of inflammation, fatty acid profiles, and production in early lactating dairy cows. Journal of Dairy Science 96 6932–6943CrossRefGoogle Scholar
Yang, L, Froio, RM, Sciuto, TE, Dvorak, AM, Alon, R & Luscinskas, FW 2005 ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium under flow. Blood 106 584–593CrossRefGoogle ScholarPubMed
Yaqoob, P & Calder, PC 1995 The effects of dietary lipid manipulation on the production of murine T cell-derived cytokines. Cytokine 7 548–553CrossRefGoogle ScholarPubMed