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Effects of FABP4 variation on milk fatty-acid composition for dairy cattle grazed on pasture in late lactation

Published online by Cambridge University Press:  31 January 2020

Yunhai Li ,
Huitong Zhou ,
Long Cheng ,
Miriam Hodge ,
Jenny Zhao ,
Rosy Tung ,
Grant Edwards  and
Jonathan Hickford
Yunhai Li
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Huitong Zhou
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Long Cheng
Affiliation:
Faculty of Veterinary and Agricultural Sciences, Dookie College, The University of Melbourne, Victoria3647, Australia
Miriam Hodge
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Jenny Zhao
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Rosy Tung
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Grant Edwards
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
Jonathan Hickford*
Affiliation:
Department of Agricultural Sciences, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
*
Author for correspondence: Jonathan Hickford, Email: [email protected]
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Abstract

This research communication describes associations between variation in the fatty acid binding protein 4 gene (FABP4) and milk fat composition in New Zealand Holstein-Friesian × Jersey cross dairy cows. After correcting for the effect of the amino acid substitution p.K232A in diacylglycerol O-acyltransferase 1 (DGAT1), which is associated with variation in many milk fatty acid (FA) component levels, the effect of FABP4 c.328A/G on milk FA levels was typically small. For the five genotypes analysed, the AB cows produced more medium-chain fatty acids than CC cows (P < 0.05), and more C14:0 FA than AA and AC cows (P < 0.05). The AA and AC cows produced less C22:0 FA (P < 0.01) than the BC cows, and the AC cows produced more C24:0 FA (P < 0.05) than was produced by the BC cows. Cows of genotype CC produce more long-chain fatty acids than cows of genotype BC (P < 0.05).

Keywords

Fatty acid binding protein 4 (FABP4) gene (FABP4)genetic variationhaplotypemilk fatty acid
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 1 , February 2020 , pp. 32 - 36
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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References

Bionaz, M and Loor, JJ (2008) ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation. The Journal of Nutrition 138(6), 1019–1024.Google ScholarPubMed
Byun, SO, Fang, Q, Zhou, H and Hickford, JGH (2009) An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Analytical Biochemistry 385(1), 174–175.Google ScholarPubMed
Dewhurst, RJ, Shingfield, KJ, Lee, MRF and Scollan, ND (2006) Increasing the concentrations of beneficial polyunsaturated fatty acids in milk produced by dairy cows in high-forage systems. Animal Feed Science and Technology 131(3), 168–206.Google Scholar
Garnsworthy, PC, Masson, LL, Lock, AL and Mottram, TT (2006) Variation of milk citrate with stage of lactation and de novo fatty acid synthesis in dairy cows. Journal of Dairy Science 89(5), 1604–1612.Google ScholarPubMed
Khatkar, MS, Thomson, PC, Tammen, I and Raadsma, H (2004) Quantitative trait loci mapping in dairy cattle: review and meta-analysis. Genetics Selection Evolution 36(2), 163–190.Google ScholarPubMed
Li, YH, Zhou, H, Cheng, L, Edwards, GR and Hickford, JGH (2019) Effect of DGAT1 variant (K232A) on milk traits and milk fat composition in outdoor pasture-grazed dairy cattle. New Zealand Journal of Agricultural Research. doi: 10.1080/00288233.2019.1589537Google Scholar
Marchitelli, C, Contarini, G, De Matteis, G, Crisà, A, Pariset, L, Scatà, M, Catillo, G, Napolitano, F and Moioli, B (2013) Milk fatty acid variability: effect of some candidate genes involved in lipid synthesis. The Journal of Dairy Research 80(2), 165–173.10.1017/S002202991300006XGoogle ScholarPubMed
Nafikov, RA, Schoonmaker, JP, Korn, KT, Noack, K, Garrick, DJ, Koehler, KJ, Minick-Bormann, J, Reecy, JM, Spurlock, DE and Beitz, DC (2013) Association of polymorphisms in solute carrier family 27, isoform A6 (SLC27A6) and fatty acid-binding protein-3 and fatty acid-binding protein-4 (FABP3 and FABP4) with fatty acid composition of bovine milk. Journal of Dairy Science 96(9), 6007–6021.10.3168/jds.2013-6703Google ScholarPubMed
Palladino, RA, Buckley, F, Prendiville, R, Murphy, JJ, Callan, J and Kenny, DA (2010) A comparison between Holstein-Friesian and Jersey dairy cows and their F1 hybrid on milk fatty acid composition under grazing conditions. Journal of Dairy Science 93(5), 2176–2184.Google Scholar
Schennink, A, Stoop, WM, Visker, MHPW, Heck, JML, Bovenhuis, H, Van Der Poel, JJ, Van Valenberg, HJF and Van Arendonk, JAM (2007) DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Animal Genetics 38(5), 467–473.Google ScholarPubMed
Schwendel, BH, Morel, PC, Wester, TJ, Tavendale, MH, Deadman, C, Fong, B, Shadbolt, NM, Thatcher, A and Otter, DE (2015) Fatty acid profile differs between organic and conventionally produced cow milk independent of season or milking time. Journal of Dairy Science 98(3), 1411–1425.Google ScholarPubMed
Soyeurt, H, Dardenne, P, Gillon, A, Croquet, C, Vanderick, S, Mayeres, P, Bertozzi, C and Gengler, N (2006) Variation in fatty acid contents of milk and milk fat within and across breeds. Journal of Dairy Science 89(12), 4858–4865.Google ScholarPubMed
Zhou, H, Hickford, JGH and Fang, Q (2006) A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification. Analytical Biochemistry 354(1), 159–161.Google ScholarPubMed
Zhou, H, Cheng, L, Azimu, W, Hodge, S, Edwards, GR and Hickford, JGH (2015) Variation in the bovine FABP4 gene affects milk yield and milk protein content in dairy cows. Scientific Reports 5, 10023.Google ScholarPubMed
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