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Effect of grazing fresh legumes or feeding silage on fatty acids and enzymes involved in the synthesis of milk fat in dairy cows

Published online by Cambridge University Press:  10 May 2010

Lars Wiking ,
Peter K Theil ,
Jacob H Nielsen  and
Martin T Sørensen
Lars Wiking*
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, Aarhus University, Research Centre Foulum, PO Box 50, DK-8830Tjele, Denmark
Peter K Theil
Affiliation:
Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, Research Centre Foulum, PO Box 50, DK-8830Tjele, Denmark
Jacob H Nielsen
Affiliation:
Department of Food Science, Faculty of Agricultural Sciences, Aarhus University, Research Centre Foulum, PO Box 50, DK-8830Tjele, Denmark
Martin T Sørensen
Affiliation:
Department of Animal Health and Bioscience, Faculty of Agricultural Sciences, Aarhus University, Research Centre Foulum, PO Box 50, DK-8830Tjele, Denmark
*
*For correspondence; e-mail: [email protected]
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Abstract

The impact of fresh legume types or silage on the composition of milk fatty acids and transcription of enzymes involved in the synthesis of milk fat in cows was studied. Three groups of cows grazed high proportions of white clover, red clover and lucerne, respectively. A fourth group of cows was fed maize/grass silage. The cows grazing high proportions of legumes produced significantly more 18:1 trans-11, 18:2 cis9-trans11, 18:2 trans10-cis12 and 18:3 fatty acids than cows fed silage. White clover and lucerne grazing resulted in significantly lower output of 18:1 trans9 in milk than red clover grazing and maize/grass silages. Transcription of stearoyl-CoA desaturase (SCD) in mammary tissue was significantly increased by grazing high proportions of legume whereas fatty acid synthase and acetyl-CoA carboxylase were not affected by type of feeding. Furthermore, average milk fat globule diameter was correlated to daily milk fat yield but was not affected by feeding. Although the fresh forage affected the transcription of SCD in mammary tissue, the largest effects were on the trans11-based fatty acids. It is concluded that type of forage, i.e. fresh or silage, had a greater impact on rumen fermentation pattern than on transcription of enzymes involved in the synthesis of milk fat.

Keywords

Conjugated linoleic acidstearoyl desaturasewhite cloverred cloverlucerne
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 3 , August 2010 , pp. 337 - 342
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Baumgard, LH, Matitashvili, E, Corl, BA, Dwyer, DA & Bauman, DE 2002 trans-10, cis-12 conjugated linoleic acid decreases lipogenic rates and expression of genes involved in milk lipid synthesis in dairy cows. Journal of Dairy Science 85 2155–2163CrossRefGoogle ScholarPubMed
Bernard, L, Leroux, C, Faulconnier, Y, Durand, D, Shingfield, KJ & Chilliard, Y 2009 Effect of sunflower-seed oil or linseed oil on milk fatty acid secretion and lipogenic gene expression in goats fed hay-based diets. Journal of Dairy Research 76 241–248CrossRefGoogle ScholarPubMed
Bernard, L, Rouel, J, Leroux, C, Ferlay, A, Faulconnier, Y, Legrand, P & Chilliard, Y 2005 Mammary lipid metabolism and milk fatty acid secretion in Alpine goats fed vegetable lipids. Journal of Dairy Science 88 1478–1489CrossRefGoogle ScholarPubMed
Brownsey, RW, Boone, AN, Elliot, JE, Kulpa, JE & Lee, WM 2006 Regulation of acetyl-CoA carboxylase. Biochemical Society Transactions 34 223–227CrossRefGoogle ScholarPubMed
Butler, G, Nielsen, JH, Slots, T, Seal, C, Eyre, MD, Sanderson, R & Leifert, C 2008 Fatty acids and fat-soluble antioxidant concentration in milk from high- and low-input conventional and organic systems: seasonal variation. Journal of the Science of Food and Agriculture 88 1430–1441CrossRefGoogle Scholar
Collomb, M, Bütikofer, UR, Sieber, B, Jeangros, B & Bosset, JO 2002 Correlation between fatty acids in cows' milk fat produced in lowlands, mountains and highland of Switzerland and botanical composition of the fodder. International Dairy Journal 12 661–666CrossRefGoogle Scholar
Couvreur, S, Hurtaud, C, Marnet, PG, Faverdin, P & Peyraud, JL 2007 Composition of milk fat from cows selected for milk fat globule size and offered either fresh pasture or a corn silage-based diet. Journal of Dairy Science 90 392–403CrossRefGoogle ScholarPubMed
Delbecchi, L, Ahnadi, CE, Kennelly, JJ & Lacasse, P 2001 Milk fatty acid composition and mammary lipid metabolism in Holstein cows fed protected or unprotected canola seeds. Journal of Dairy Science 84 1375–1381CrossRefGoogle ScholarPubMed
Deeney, JT, Valivullah, HM, Dapper, CH, Dylewski, DP & Keenan, TW 1985 Microlipid droplets in milk secreting mammary epithelial cells: evidence that they originate from endoplasmic reticulum and are precursors of milk lipid globules. European Journal of Cell Biology 38 16–26Google ScholarPubMed
Dewhurst, RJ, Fisher, WJ, Tweed, JKS & Wilkins, RJ 2003a Comparison of grass and legume silages for milk production. 2. In vivo and in sacco evaluations of rumen function. Journal of Dairy Science 86 2598–2611CrossRefGoogle ScholarPubMed
Dewhurst, RJ, Evans, RT, Scolland, ND, Moorby, JM, Merry, RJ & Wilkins, RJ 2003b Comparison of grass and legume silages for milk production. 1. Production response with different levels of concentrate. Journal of Dairy Science 86 2612–2621CrossRefGoogle ScholarPubMed
Garnsworthy, PC, Masson, LL, Lock, AL & Mottram, TT 2006 Variation of milk citrate with stage of lactation and de novo fatty acid synthesis in dairy cows. Journal Dairy Science 89 1604–1612CrossRefGoogle ScholarPubMed
Jensen, RG 2002 The composition of bovine milk lipids: January 1995 to December 2000. Journal of Dairy Science 85 295–350CrossRefGoogle ScholarPubMed
Knudsen, J 1972 Fatty acid synthetase from cow mammary gland tissue cells. Biochimica Biophysica Acta 280 408–414CrossRefGoogle ScholarPubMed
Nørgaard, JV, Baumgard, LH, Nielsen, TS, Theil, PK, Sørensen, MT & Sejersen, K 2008 Mammary cell turnover and expression of lipogenic genes in response to silage induces CLA and diet energy density in lactating cows. Acta Agriculturae Scandinavica A 58 113–120Google Scholar
Ortis-Gonzalez, G, Jimenz-Flores, R, Bremmes, DR, Clark, JH, DePeters, EJ, Schimdt, SJ & Drackley, JK 2007 Functional properties of butter oil made from bovine milk with experimentally altered fat composition. Journal of Dairy Science 90 5018–5031CrossRefGoogle Scholar
Paton, CM & Ntambi, JM 2009 Biochemical and physiological function of stearoyl-CoA desaturase. American Journal of Physiology Endocrinology and Metabolism 297 E28–37CrossRefGoogle ScholarPubMed
Peterson, DG, Matitashvili, EA & Bauman, DE 2003 Diet-induced milk fat depression in dairy cows results in increased trans-10, cis-12 CLA in milk fat and coordinate suppression of mRNA abundance for mammary enzymes involved in milk fat synthesis. Journal of Nutrition 133 3098–3102CrossRefGoogle ScholarPubMed
Poulsen, KA, Young, JF, Theil, P, Kolko, M, Oksbjerg, N & Lambert, N 2006 Role of phospholipase A2 in the induction of drip loss in pork. Journal of Agricultural and Food Chemistry 55 1970–1976CrossRefGoogle Scholar
Slots, T, Butler, G, Leifert, C, Kristensen, T, Skibsted, LH & Nielsen, JH 2009 Potentials to differentiate milk composition by different feeding strategies. Journal of Dairy Science 92 2057–2066CrossRefGoogle ScholarPubMed
Smith, S 1994 The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB Journal 8 1248–1259CrossRefGoogle ScholarPubMed
Sorensen, MT, Nørgaard, JV, Theil, PK, Vestergaard, M & Sejrsen, K 2006 Cell turnover and activity in mammary tissue during lactation and the dry period in dairy cows. Journal of Dairy Science 89 4632–4639CrossRefGoogle ScholarPubMed
Soyeurt, H, Dehareng, F, Mayeres, P, Bertozzi, C & Gengler, N 2008 Variation of Δ9-desaturase activity in dairy cattle. Journal of Dairy Science 91 3211–3224CrossRefGoogle Scholar
Timmen, H & Patton, S 1988 Milk fat globules: Fatty acid composition, size and in vivo regulation of fat liquidity. Lipids 23 685–689CrossRefGoogle ScholarPubMed
Theil, PK, Sørensen, IL, Therkildsen, M & Oksbjerg, N 2006 Changes in proteolytic enzyme mRNAs relevant for meat quality during myogenesis of primary porcine satellite cell. Meat Science 73 335–343CrossRefGoogle Scholar
Van Dorland, HA, Kreuzer, M, Leuenberger, H & Wettstein, H-R 2008 Comparative potential of white and red clover to modify the milk fatty acid profile of cows fed ryegrass-based diets from zero-grazing and silage systems. Journal of the Science of Food and Agriculture 88 75–85CrossRefGoogle Scholar
Weisbjerg, MR, Wiking, L, Kristensen, NB and Lund, P 2008 Effects of supplemental dietary fatty acids on milk yield and fatty acid composition in high and medium yielding cows. Journal of Dairy Research 75 142–152CrossRefGoogle ScholarPubMed
Whiting, CM, Mutsvangwa, T, Walton, JP, Cant, JP & McBride, BW 2004 Effects of feeding either fresh alfalfa or alfalfa silage on milk fatty acid content in Holstein dairy cows. Animal Feed Science and Technology 113 27–37CrossRefGoogle Scholar
Wiking, L, Björck, L & Nielsen, JH 2003 The influence of feed on stability of fat globules during pumping of raw milk. International Dairy Journal 13 797–803CrossRefGoogle Scholar
Wiking, L, Stagsted, J, Björck, L & Nielsen, JH 2004 Milk fat globule size is affected by fat production in dairy cows. International Dairy Journal 14 909–913CrossRefGoogle Scholar