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Effect of g.9476869G>A myeloperoxidase (MPO) gene polymorphism on the antioxidant activity of milk from Polish Holstein-Friesian cows of the Black-and-White variety (HO)

Published online by Cambridge University Press:  19 May 2017

Joanna Pokorska ,
Karolina Poskart ,
Dominika Kułaj ,
Andrzej Ochrem ,
Magdalena Dusza ,
Zygmunt Gil ,
Ewa Świętek  and
Joanna Makulska
Joanna Pokorska*
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Karolina Poskart
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Dominika Kułaj
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Andrzej Ochrem
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Magdalena Dusza
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Zygmunt Gil
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Ewa Świętek
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
Joanna Makulska
Affiliation:
Department of Cattle Breeding, Institute of Animal Sciences, University of Agriculture in Cracow, Poland
*
*For correspondence; e-mail: [email protected]
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Abstract

Myeloperoxidase (MPO) is an important enzyme, which is one of the components of the antibacterial system in neutrophils and monocytes. MPO participates in the inflammatory response in multiple locations in the body, including the mammary glands. As a result of the activity of MPO, many oxidising compounds as well as reactive oxygen species are generated. It seems that myeloperoxidase may be a marker linking inflammation processes and oxidative stress. So far, there are no literature data on the association between the MPO gene polymorphism and the antioxidant properties of milk. The aim of the study was to analyse the effect of g.9476869G > A polymorphism of myeloperoxidase (MPO) gene and age of cows on the antioxidant activity of milk and other milk traits in Polish Holstein-Friesian cows. Polymorphism of MPO gene was identified by the PCR-RFLP method using the HphI endonuclease. The total antioxidant capacity of milk samples was measured by the Trolox Equivalent Antioxidant Capacity (TEAC) method. It was found that the GG genotype was the most frequent (0·606). The genotype at the tested MPO locus and the age of the animals affected the antioxidant activity of milk. Milk from cows with the GA genotype was characterised by a significantly higher antioxidant activity than milk from cows with the GG genotype (P < 0·0001). The analysis of interaction showed that cows with the GA genotype and older than 6·5 years produced milk with a significantly higher antioxidant activity compared with younger cows with the same genotype (P < 0·0001), as well as cows with the GG genotype of all ages (P < 0·0001).

Keywords

Myeloperoxidasemilk antioxidant activitygene polymorphism
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 2 , May 2017 , pp. 159 - 164
Copyright
Copyright © Proprietors of Journal of Dairy Research 2017 

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