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Effect of coagulase-negative staphylococci on somatic cell count in Dutch dairy herds

Published online by Cambridge University Press:  10 May 2010

Otlis Sampimon ,
Bart HP van den Borne ,
Inge Santman-Berends ,
Herman W Barkema  and
Theo Lam
Otlis Sampimon*
Affiliation:
GD Animal Health Service, Deventer, The Netherlands
Bart HP van den Borne
Affiliation:
Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
Inge Santman-Berends
Affiliation:
GD Animal Health Service, Deventer, The Netherlands
Herman W Barkema
Affiliation:
Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada
Theo Lam
Affiliation:
GD Animal Health Service, Deventer, The Netherlands Dutch Udder Health Centre, Deventer, The Netherlands
*
*For correspondence; e-mail: [email protected]
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Abstract

The effect was quantified of coagulase-negative staphylococci (CNS) intramammary infections on quarter- and cow-level somatic cell count (SCC) and on bulk milk somatic cell count (BMSCC) in different BMSCC cohorts in Dutch dairy herds. Two datasets were used for this purpose. In the first dataset, on 49 randomly selected dairy farms a total of 4220 quarter milk samples of 1072 cows were collected of all cows and heifers with a test-day SCC ⩾250 000 and ⩾150 000 cells/ml, respectively, and of 25% of cows and heifers below these thresholds. In the second dataset, on 39 selected dairy farms a total of 8329 quarter milk samples of 2115 cows were collected of all cows with a test-day SCC ⩾250 000 cells/ml following two consecutive SCC <250 000 cells/ml, and of heifers using the same SCC criteria but with a threshold of 150 000 cells/ml. These cows and heifers were defined as new high SCC. In both datasets, CNS was the most frequently isolated pathogen, 11% in the first dataset and 12% in the second dataset. In both datasets, quarters with CNS IMI had a lower SCC than quarters infected with major pathogens, and a higher SCC than culture-negative quarters. The same was found for SCC at cow level. Coagulase-negative staphylococci were more often found in quarters with SCC ⩾200 000 cells/ml in dairy farms with a BMSCC <150 000 cells/ml compared with dairy farms with a higher BMSCC. Prevalence of CNS in cows and heifers with a high SCC was higher in dairy farms with a BMSCC <150 000 cells/ml compared with dairy farms with a medium or high BMSCC: 30, 19 and 18%, respectively. This indicates that CNS IMI as a cause of subclinical mastitis is relatively more important in dairy farms with a low BMSCC and may become a point of attention in udder health management on that type of farm.

Keywords

Coagulase-negative staphylococciminor pathogensmastitissomatic cell count
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 3 , August 2010 , pp. 318 - 324
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Barkema, HW, Schukken, YH, Lam, TJGM, Galligan, DT, Beiboer, ML & Brand, A 1997 Estimation of interdependence among quarters of the bovine udder with subclinical mastitis and implications for analysis. Journal of Dairy Science 80 1592–1599CrossRefGoogle ScholarPubMed
Boddie, RL, Nickerson, SC, Owens, WE & Watts, JL 1987 Udder microflora in nonlactating heifers. Agri-Practice 8 22–25Google Scholar
Borne, van den BHP, Schaik, van G, Lam, TJGM & Nielen, M 2009 Bacteriological cure of recently acquired subclinical mastitis following antimicrobial treatment in lactating Dutch dairy cows. In: Proceedings of the 12th Conference of the International Society for Veterinary Epidemiology and Economics, Durban, South AfricaGoogle Scholar
Bradley, AJ, Leach, KA, Breen, JE, Green, LE & Green, MJ 2007 Survey of the incidence and aetiology of mastitis on dairy farms in England and Wales. Veterinary Record 160 253–258CrossRefGoogle ScholarPubMed
Chaffer, M, Leitner, G, Winkler, M, Glickman, A, Krifucks, O, Ezra, E & Saran, A 1999 Coagulase-negative staphylococci and mammary gland infections in cows. Zentralblatt fur Veterinarmedicine B 46 707–712Google ScholarPubMed
Djabri, B, Bareille, N, Beaudeau, F & Seegers, H 2002 Quarter milk somatic cell count in infected dairy cows—a meta-analysis. Veterinary Research 33 335–357CrossRefGoogle Scholar
Gillespie, BE, Headrick, SI, Boonyayatra, S & Oliver, SP 2009 Prevalence of coagulase-negative Staphylococcus species in three dairy research herds. Veterinary Microbiology 134 65–72CrossRefGoogle ScholarPubMed
Harmon, RJ, Eberhart, RJ, Jasper, DE, Langlois, BE & Wilson, RA 1990 Microbiological Procedures for the Diagnosis of Udder Infection. Arlington VA, USA: National Mastitis Council Inc.Google Scholar
Honkanen-Buzalski, T 1990 The role of coagulase-negative Staphylococcus species in bovine mastitis. In: Proceedings 23rd Annual Conference of the American Association of Bovine Practitioners, Indianapolis IN, USA, pp. 98–102Google Scholar
Jarp, J 1991 Classification of coagulase-negative staphylococci isolated from bovine clinical and subclinical mastitis. Veterinary Microbiology 27 151–158CrossRefGoogle ScholarPubMed
Lam, TJGM, van Wuijckhuise, LA, Franken, P, Morselt, ML, Hartman, EG & Schukken, YH 1996 Use of composite milk samples for diagnosis of Staphylococcus aureus mastitis in dairy cattle. Journal of American Veterinary Medical Association 208 1705–1708CrossRefGoogle ScholarPubMed
Makovec, JA & Ruegg, PL 2003 Results of milk samples submitted for microbiological examination in Wisconsin from 1994 to 2001. Journal of Dairy Science 86 3466–3472CrossRefGoogle ScholarPubMed
Matos, JS, White, DG, Harmon, RJ & Langlois, BE 1991 Isolation of Staphylococcus aureus from sites other than the lactating mammary gland. Journal of Dairy Science 74 1544–1549CrossRefGoogle Scholar
Neave, FK, Dodd, FH, Kingwill, RG & Westgarth, DR 1969 Control of mastitis in the dairy herd by hygiene and management. Journal of Dairy Science 52 696–707CrossRefGoogle ScholarPubMed
Piepers, SL, De Meulemeester, L, De Kruif, A, Opsomer, G, Barkema, HW & De Vliegher, S 2007 Prevalence and distribution of mastitis pathogens in subclinically infected dairy cows in Flanders, Belgium. Journal of Dairy Research 74 478–483CrossRefGoogle ScholarPubMed
Pitkälä, A, Haveri, M, Pyörälä, S, Myllys, V & Honkanen-Buzalski, T 2004 Bovine mastitis in Finland 2001 – prevalence, distribution of bacteria, and antimicrobial resistance. Journal of Dairy Science 87 2433–2441CrossRefGoogle ScholarPubMed
Rainard, P, Ducelliez, M & Poutrel, B 1990 The contribution of mammary infections by coagulase-negative staphylococci to the herd bulk milk somatic cell count. Veterinary Research Communications 14 193–198CrossRefGoogle Scholar
Sampimon, OC, Barkema, HW, Berends, IMGA, Sol, J & Lam, TJGM 2009a Prevalence of intramammary infection in Dutch dairy herds. Journal of Dairy Research 76 129–136CrossRefGoogle ScholarPubMed
Sampimon, OC, Barkema, HW, Berends, IMGA, Sol, J & Lam, TJGM 2009b Prevalence and herd-level risk factors for intramammary infection with coagulase-negative staphylococci in Dutch dairy herds. Veterinary Microbiology 134 37–44CrossRefGoogle ScholarPubMed
Schukken, YH, Wilson, DJ, Welcome, F, Tikofsky, L & Gonzalez, RN 2003 Monitoring udder health and milk quality using somatic cell count. Veterinary Research 34 579–596CrossRefGoogle Scholar
Schukken, YH, González, RN, Tikofsky, LL, Schulte, HF, Santisteban, CG, Welcome, FL, Bennett, GJ, Zurakowski, MJ & Zadoks, RN 2009 CNS mastitis: Nothing to worry about? Veterinary Microbiology 134 9–14CrossRefGoogle ScholarPubMed
Sears, PM, Smith, BS, English, PB, Herer, PS & Gonzalez, RN 1990 Shedding pattern of Staphylococcus aureus from intramammary infections. Journal of Dairy Science 73 2785–2789CrossRefGoogle ScholarPubMed
Simojoki, H, Orro, T, Taponen, S & Pyörälä, S 2009 Experimental model of bovine clinical mastitis caused by Staphylococcus chromogenes. Veterinary Microbiology 134 95–99CrossRefGoogle ScholarPubMed
Stabenfeldt, GH & Spencer, GR 1966 The lesions in bovine udders shedding nonhemolytic coagulase-negative staphylococci. Pathology Veterinary 3 27–39CrossRefGoogle ScholarPubMed
Taponen, S, Simojoki, H, Haveri, M, Larsen, HD & Pyörälä, S 2006 Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Veterinary Microbiology 115 199–207CrossRefGoogle ScholarPubMed
Taponen, S, Koort, J, Björkroth, J, Saloniemi, H & Pyörälä, S 2007 Bovine intramammary infections caused by coagulase-negative staphylococci may persist throughout lactation according to amplified fragment length polymorphism-based analysis. Journal of Dairy Science 90 3301–3307CrossRefGoogle ScholarPubMed
Tenhagen, BA, Koster, G, Wallmann, J & Heuwieser, W 2006 Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. Journal of Dairy Science 89 2542–2551CrossRefGoogle ScholarPubMed
White, DG, Harmon, RJ, Matos, JES & Langlois, BE 1989 Isolation and identification of coagulase-negative Staphylococcus species from bovine body sites and streak canals of nulliparous heifers. Journal of Dairy Science 72 1886–1892CrossRefGoogle ScholarPubMed
Zadoks, RN & Watts, JL 2009 Species identification of coagulase-negative staphylococci: genotyping is superior to phenotyping. Veterinary Microbiology 134 20–28CrossRefGoogle ScholarPubMed