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Effect of a pre-milking teat foam and a liner disinfectant on the presence of mesophilic and (proteolytic) psychrotrophic bacteria prior to milking

Published online by Cambridge University Press:  26 November 2019

M. Verhegghe ,
J. De Block ,
S. Van Weyenberg ,
L. Herman ,
M. Heyndrickx  and
E. Van Coillie
M. Verhegghe
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090Melle, Belgium
J. De Block
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090Melle, Belgium
S. Van Weyenberg
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090Melle, Belgium
L. Herman
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090Melle, Belgium
M. Heyndrickx
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090Melle, Belgium Ghent University, Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Salisburylaan 133, 9820Merelbeke, Belgium
E. Van Coillie*
Affiliation:
Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090Melle, Belgium
*
Author for correspondence: E. Van Coillie, Email: [email protected]
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Abstract

Contamination of raw milk by psychrotrophs can lead to the production of heat-resistant proteases and subsequent spoilage of UHT milk. Therefore, this research communication evaluated the effect of a pre-milking teat disinfectant (active components: L-(+)-lactic acid and salicylic acid) and a liner disinfectant (active components: peracetic acid and hydrogen peroxide) on the number of mesophilic and (proteolytic) psychrotrophic bacteria prior to milking. The teat orifices of 10 cows were sampled using a swabbing procedure before and after treatment with a pre-milking teat disinfectant on six subsequent days. On the teat orifices, there was a small but statistically significant decrease in the psychrotrophic bacterial counts between pre and post dipping. No differences were observed for the mesophilic bacterial counts and proteolytic active counts. Liners were also sampled using swabs pre and post disinfection. No statistically significant decrease in the bacterial counts was observed post liner disinfection, although there was a numerical decrease. Sixty-two percent of the proteolytic psychrotrophs were pseudomonads: 16.5% of which were P. fragi, 14.3% P. lundensis, 10.0% P. fluorescens and 2.9% P. putida. Trinitrobenzenesulfonic acid (TNBS) analysis revealed a wide variety in proteolytic activity (from 0 to 55 µmol glycine/ml milk) and the presence of high producers. It can be concluded that there was only a minor effect of teat and liner disinfection on the psychrotrophic bacterial counts indicating that the measures presented did not result in a reduction of the targeted bacteria on teat orifices and liners.

Keywords

(Proteolytic) psychrotrophic bacteriadisinfectantsmilking devicePseudomonasudder
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 4 , November 2019 , pp. 432 - 435
Copyright
Copyright © Hannah Dairy Research Foundation 2019

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References

De Jonghe, V, Coorevits, A, Van Hoorde, K, Messens, W, Van Landschoot, A, De Vos, P and Heyndrickx, M (2011) Influence of storage conditions on the growth of Pseudomonas species in refrigerated raw milk. Applied and Environmental Microbiology 77, 460–470.CrossRefGoogle ScholarPubMed
Fitzpatrick, S, Garvey, M and Gleeson, D (2018) A review of test protocols for the evaluation of teat disinfectants. International Journal of Dairy Technology 71, 553–563.CrossRefGoogle Scholar
Guarin, JF, Baumberger, C and Ruegg, PL (2017) Anatomical characteristics of teats and premilking bacterial counts of teat skin swabs of primiparous cows exposed to different types of bedding. Journal of Dairy Science 100, 1436–1444.CrossRefGoogle ScholarPubMed
Marchand, S (2009) Identification and characterization of milk spoiling Pseudomonas spp. and their heat resistant proteases: impact on the shelf life of UHT milk. Thesis submitted in fulfillment of the requirements of the degree of Doctor (PhD) in Applied Biological Sciences. Faculty of Bioscience Engineering, Ghent University.Google Scholar
Nucera, DM, Lomonaco, S, Morra, P, Ortoffi, MF, Giaccone, D and Grassi, MA (2016) Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms. Italian Journal of Food Safety 5, 91–94.CrossRefGoogle ScholarPubMed
Verdier-Metz, I, Gagne, G, Bornes, S, Monsallier, F, Veisseire, P, Delbès-Paus, P and Montela, MC (2012) Cow teat skin, a potential source of diverse microbial populations for cheese production. Applied and Environmental Microbiology 78, 326–333.CrossRefGoogle ScholarPubMed
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