Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-27T23:24:24.689Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of a biological response modifier on cellular death mechanisms at drying off

Published online by Cambridge University Press:  12 May 2008

Bibliana E Dallard ,
Celina Baravalle ,
Hugo Ortega ,
Valeria Ruffino ,
Silvia Heffel  and
Luis F Calvinho
Bibliana E Dallard
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Celina Baravalle
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Hugo Ortega
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Valeria Ruffino
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Silvia Heffel
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina
Luis F Calvinho
Affiliation:
Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Rvdo. Padre Kreder 2805, (3080) Esperanza, Santa Fe, Argentina Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria, C.C. 22 (2300) Rafaela, Santa Fe, Argentina
Get access Rights & Permissions [Opens in a new window]

Abstract

Agents that increase natural protective mechanisms have been proposed for prevention and treatment of intramammary infections. The objective of this study was to describe the effects of a single intramammary infusion of a lipopolysaccharide (LPS)-based biological response modifier (BRM) on cellular death mechanism in uninfected and Staphylococcus aureus-infected bovine mammary glands during involution. Three groups of 12 cows, each one including 6 Staph. aureus-infected and 6 uninfected, were infused in two mammary quarters with BRM or placebo and slaughtered at 7, 14 and 21 d of involution. In infected quarters, BRM treatment produced a significant increase in percent of stained epithelial cells for the apoptosis-promoting protein Bax at every observation period. In addition, BRM produced a significant increase of immunostained stromal cells for Bax compared with placebo-treated quarters. BRM treatment produced an increase in percentages of epithelial cells staining with active caspase-3 at 7 d and 14 d of involution compared with placebo-treated quarters and a significant decrease in percentages of terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive epithelial cells at 7 d and 21 d of involution. In addition, BRM treatment caused an increase in percentage of stromal cells immunostaining for active caspase-3 and TUNEL. An increase of active caspase-3 and TUNEL epithelial and stromal cell immunostaining was observed in Staph. aureus-infected compared with uninfected quarters. Cellular proliferation, determined by Ki-67 immunostaining, was increased in epithelial and stromal cells from Staph. aureus-infected compared with uninfected quarters at every observation period. These results provide new insights into the mechanism of mammary cell death in uninfected and Staph. aureus-infected bovine mammary gland during involution and illustrate the effects of LPS-based BRM on apoptosis and cell proliferation during mammary involution.

Keywords

Biological response modifiermammary gland involutionStaphylococcus aureusapoptosis
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 2 , May 2008 , pp. 167 - 175
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Calvinho, LF, Ortega, HH, Dallard, BB, Iguzquiza, IA, Salvetti, NR & Quaino, OA 2004 Effect of intramammary infusion of a biological response modifier in Holstein cows at the end of lactation. Abstr. 409.00308 In Proceedings of XIXth Panamerican Congress of Veterinary Science, Buenos Aires, ArgentinaGoogle Scholar
Capuco, AV, Li, M, Long, E, Ren, S, Hruska, KS, Schorr, K & Furth, PA 2002 Concurrent pregnancy retards mammary involution: effects on apoptosis and proliferation of the mammary epithelium after forced weaning of mice. Biology of Reproduction 66 1471–1476CrossRefGoogle ScholarPubMed
Colitti, M, Wilde, CJ & Stefanon, B 2004 Functional expression of Bcl-2 protein family and AIF in bovine mammary tissue in early lactation. Journal of Dairy Research 71 20–27CrossRefGoogle ScholarPubMed
Dallard, BE, Ruffino, V, Heffel, S & Calvinho, LF 2007 Effect of a biological response modifier on expression of growth factors and cellular proliferation at drying off. Journal of Dairy Science 90 2229–2240CrossRefGoogle ScholarPubMed
du Manoir, S, Guillaud, P, Camus, E, Seigneurin, D & Brugal, G 1991 Ki-67 labeling in postmitotic cells defines different Ki-67 pathways within the 2c compartment. Cytometry 12 455–463CrossRefGoogle ScholarPubMed
Gerdes, J, Lemke, H, Baisch, H, Wacker, HH, Schwab, U & Stein, H 1984 Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. Journal of Immunology 133 1710–1705CrossRefGoogle ScholarPubMed
Hall, PA, Coates, PJ, Ansari, B & Hopwood, D 1994 Regulation of cell number in the mammalian gastrointestinal tract: the importance of apoptosis. Journal of Cell Science 107 3569–3577CrossRefGoogle ScholarPubMed
Heermeier, K, Benedict, M, Li, M, Furth, P, Nunez, G & Hennighausen, L 1996 Bax and Bcl-xs are induced at the onset of apoptosis in involuting mammary epithelial cells. Mechanisms of Development 56 197–207CrossRefGoogle ScholarPubMed
Hogan, JS, Harmon, RJ, González, RN, Nickerson, SC, Oliver, SP, Pankey, JW & Smith, KL 1999 Laboratory handbook on bovine mastitis. Madison WI, USA: National Mastitis Council, p. 222Google Scholar
Long, E, Capuco, AV, Wood, DL, Sonstegard, T, Tomita, G, Paape, MJ & Zhao, X 2001 Escherichia coli induces apoptosis and proliferation of mammary cells. Cell Death and Differentiation 8 808–816CrossRefGoogle ScholarPubMed
Löhr, CV, Teifke, JP, Failing, K & Weiss, E 1997 Characterization of the proliferation state in canine mammary tumors by the standardized AgNoR method with postfixation and immunologic detection of Ki-67 and PCNA. Veterinary Pathology 34 212–221CrossRefGoogle Scholar
Mahajan, NP, Linder, K, Berry, G, Gordon, GW, Heim, R & Herman, B 1998 Bcl-2 and Bax interactions in mitochondria probed with green fluorescent protein and fluorescence resonance energy transfer. Nature Biotechnology 16 547–552CrossRefGoogle ScholarPubMed
Merlo, GR, Graus-Porta, D, Cella, N, Marte, BM, Taverna, D & Hynes, NE 1996 Growth, differentiation and survival of HC11 mammary epithelial cells: diverse effects of receptor tyrosine kinase-activating peptide growth factors. European Journal of Cell Biology 70 97–105Google ScholarPubMed
Motyl, T, Gajkowska, B, Wojewodzka, U, Wareski, P, Rekiel, A & Ploszaj, T 2001 Expression of apoptosis-related proteins in involuting mammary gland of sow. Comparative Biochemistry & Physiology B 128 635–646CrossRefGoogle ScholarPubMed
Nickerson, SC, Owens, WE, Boddie, RL & Boddie, NT 1992 The effect of chronic immunostimulation of the nonlactating bovine mammary gland with interleukin-2, pokeweed mitogen and lipopolysaccharide. Journal of Dairy Science 75 3339–3351CrossRefGoogle ScholarPubMed
Oliver, SP & Smith, KL 1982a Bovine mammary involution following intramammary infusion of colchicine and endotoxin at drying off. Journal of Dairy Science 65 801–813CrossRefGoogle ScholarPubMed
Oliver, SP & Smith, KL 1982b Nonantibiotic approach in control of bovine mastitis during dry period. Journal of Dairy Science 65 2119–2124CrossRefGoogle ScholarPubMed
Oliver, SP & Sordillo, LM 1988 Udder health in the periparturient period. Journal of Dairy Science 71 2584–2606CrossRefGoogle ScholarPubMed
Ortega, HH, Salvetti, NR, Amable, P, Dallard, BE, Baravalle, C, Barbeito, CG & Gimeno, EJ 2007 Intraovarian localization of growth factors in induced cystic ovaries in rats. Anatomia, Histologia, Embryologia: Journal of Veterinary Medicine Series C 36 94–102CrossRefGoogle ScholarPubMed
Osborn, MJ 1963 Studies on the Gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium. Proceedings of the National Academy of Sciences of USA 50 499–506CrossRefGoogle ScholarPubMed
Quarrie, L, Addey, C & Wilde, C 1996 Programmed cell death during mammary involution induced by weaning, litter removal and milk stasis. Journal of Cellular Physiology 168 559–5693.0.CO;2-O>CrossRefGoogle ScholarPubMed
Quarrie, LH, Addey, CV & Wilde, CJ 1994 Local regulation of mammary apoptosis in the lactating goat. Biochemical Society Transactions 22 178–185CrossRefGoogle ScholarPubMed
Reed, CJ 1997 Cytochrome c: Can't live with it – Can't live without it. Cell 91 559–562CrossRefGoogle Scholar
Reed, CJ 2000 Mechanism of apoptosis. American Journal of Pathology 157 1415–1430CrossRefGoogle Scholar
SAS Institute 1999 SAS OnlineDoc®, Version 8, Cary NC, USA: SAS Institute Inc.Google Scholar
Schorr, K, Li, M, Krajewski, S, Reed, JC & Furth, PA 1999 Bcl-2 gene family and related proteins in mammary gland involution and breast cancer. Journal of Mammary Gland Biology and Neoplasia 4 153–164CrossRefGoogle ScholarPubMed
Sheffield, LG 1997 Mastitis increases growth factor messenger ribonucleic acid in bovine mammary glands. Journal of Dairy Science 80 2020–2024CrossRefGoogle ScholarPubMed
Takahashi, H, Odai, M, Mitani, K, Inumaru, S, Arai, S, Horino, R & Yokomizo, Y 2004 Effect of intramammary injection of rboGM-CSF on milk levels of chemiluminiscence activity, somatic cell count, and Staphylococcus aureus count in Holstein cows with S. aureus subclinical mastitis. Canadian Journal of Veterinary Research 68 182–187Google Scholar
Thornberry, NA & Lazebnik, Y 1998 Caspases: enemies within. Science 281 1312–1316CrossRefGoogle Scholar
Tzianabos, AO 2000 Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function. Clinical Microbiology Reviews 13 523–533CrossRefGoogle ScholarPubMed
Van Miert, ASJPAM 1991 Acute phase response and non cellular defence mechanisms. Flemish Veterinary Journal 62 69–72Google Scholar
Wareski, P, Motyl, T, Ryniewicz, Z, Orzechowski, A, Gajkowska, B, Wojewodzka, U & Ploszaj, T 2001 Expression of apoptosis-related proteins in mammary gland of goat. Small Ruminant Research 40 279–289CrossRefGoogle ScholarPubMed
Wesson, CA, Deringer, J, Liou, LE, Bayles, KW, Bohach, GA & Trumble, WR 2000 Apoptosis induced by Staphylococcus aureus in epithelial cells utilizes a mechanism involving caspases 8 and 3. Infection and Immunity 68 2998–3001CrossRefGoogle Scholar
Wilde, CJ, Addei, CV, Li, P & Fenig, DG 1997 Programmed cell death in bovine mammary tissue during lactation and involution. Experimental Physiology 82 943–953CrossRefGoogle ScholarPubMed
Zhang, H, Heim, J & Meyhack, B 1998 Redistribution of bax from cytosol to membranes is induced by apoptotic stimuli and is an early step in the apoptotic pathway. Biochemical and Biophysical Research Communication 251 454–459CrossRefGoogle ScholarPubMed
Zecconi, A 2000 Present and future of modulation of mammary gland immunity. In Proceedings IDF Symposium on Immunology of Ruminant Mammary Gland (Ed. Zecconi, A.), pp. 397–402Google Scholar