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The dynamics of individual whey protein concentrations in cows’ mammary secretions during the colostral and early lactation periods

Published online by Cambridge University Press:  06 December 2018

Raquel F.S. Raimondo ,
Juliana S.P. Ferrão ,
Samantha I. Miyashiro ,
Priscila T. Ferreira ,
João Paulo E. Saut ,
Daniela B. Birgel  and
Eduardo H. Birgel Junior
Raquel F.S. Raimondo*
Affiliation:
Núcleo RuminAção, ensino, pesquisa e extensão em ruminantes. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, 90040-060, Brazil
Juliana S.P. Ferrão
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Sao Paulo, Brazil
Samantha I. Miyashiro
Affiliation:
Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Sao Paulo, Brazil
Priscila T. Ferreira
Affiliation:
Núcleo RuminAção, ensino, pesquisa e extensão em ruminantes. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre - RS, 90040-060, Brazil
João Paulo E. Saut
Affiliation:
Large Animal Health Laboratory, Faculty of Veterinary Medicine, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
Daniela B. Birgel
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Sao Paulo, Brazil
Eduardo H. Birgel Junior
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Sao Paulo, Brazil
*
Authors for correspondence: Raquel F.S. Raimondo, Email: [email protected]
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Abstract

The bovine whey consists of more than 200 different types of proteins, of which β-lactoglobulin, α-lactalbumin, serum albumin, immunoglobulins and lactoferrin predominate. However, their concentrations are not stable due to the existence of protein dynamics during a transition from colostrum secretion to mature milk. To evaluate the dynamics of whey proteins of Jersey cows during a colostral phase and first month of lactation and an influence of the number of lactations, 268 milk samples from 135 Jersey cows were selected through a clinical evaluation. Whey was obtained by rennet coagulation of the mammary secretion. The concentration of total proteins was determined by the biuret method and their fractions were identified by 12% dodecyl sulfate-polyacrylamide gel electrophoresis (12% SDS-PAGE). Maximum concentrations of all protein fractions were observed in the first 12 h of lactation, reducing over the course of the study. Modification of the protein predominance was also observed. The transition from colostrum secretion to milk occurred between 24 and 72 h postpartum. There was an influence of the number of lactations on the dynamics of whey proteins, indicating that multiparous cows had better immunological and nutritional quality when compared to primiparous cows.

Keywords

Alpha-lactalbumincolostrumimmunoglobulinSDS-PAGEwhey
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 1 , February 2019 , pp. 88 - 93
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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