Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-09T22:34:28.207Z Has data issue: false hasContentIssue false
  • English
  • Français

Disulphide arrangement in bovine caseins: localization of intrachain disulphide bridges in monomers of κ- and αs2-casein from bovine milk

Published online by Cambridge University Press:  01 June 2009

Lone K. Rasmussen ,
Peter Højrup  and
Torben E. Petersen
Lone K. Rasmussen
Affiliation:
Protein Chemistry Laboratory, The Science Park, University of Aarhus, DK-8000 Aarhus C, Denmark
Peter Højrup
Affiliation:
Protein Chemistry Laboratory, The Science Park, University of Aarhus, DK-8000 Aarhus C, Denmark
Torben E. Petersen
Affiliation:
Protein Chemistry Laboratory, The Science Park, University of Aarhus, DK-8000 Aarhus C, Denmark
Get access Rights & Permissions [Opens in a new window]

Summary

Naturally occurring monomeric κ-casein and αs2-casein in bovine milk were purified by ion-exchange chromatography in order to localize potential intrachain disulphide bridges. Enzymic cleavage of the proteins followed by mass spectrometry and amino acid sequence analysis of cystine-containing peptides revealed the presence of an intrachain disulphide bond in both proteins.

Type
Original articles
Information
Journal of Dairy Research , Volume 61 , Issue 4 , November 1994 , pp. 485 - 493
Copyright
Copyright © Proprietors of Journal of Dairy Research 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andrews, A. T., Taylor, M. D. & Owen, A. J. 1985 Rapid analysis of bovine milk proteins by fast protein liquid chromatography. Journal of Chromatography 348 177–185CrossRefGoogle ScholarPubMed
Annan, W. D. & Manson, W. 1969 A fractionation of the αs-casein complex of bovine milk. Journal of Dairy Research 36 259–268CrossRefGoogle Scholar
Barkholt, V. & Jensen, A. L. 1989 Amino acid analysis: determination of eysteine plus half-cystine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive. Analytical Biochemistry 177 318–322CrossRefGoogle Scholar
Brignon, G., Ribadeau Dumas, B., Mercier, J.-C., Pélissier, J.-P. & Das, B. C. 1977 Complete amino acid sequence of bovine αs2-casein. FEBS Letters 76 274–279CrossRefGoogle ScholarPubMed
Carroll, R. J. & Farrell, H. M. 1983 Immunological approach to location of κ-casein in the casein micelle by electron microscopy. Journal of Dairy Science 66 679–686CrossRefGoogle Scholar
Dalgleish, D. G. 1986 Analysis by fast protein liquid chromatography of variants of κ-casein and their relevance to micellar structure and renneting. Journal of Dairy Research 53 43–51CrossRefGoogle ScholarPubMed
Griffin, M. C. A. & Roberts, G. C. K. 1985 A 1H-n.m.r. study of casein micelles. Biochemical Journal 228 273–276CrossRefGoogle ScholarPubMed
Hirs, C. H. W. 1967 Performic acid oxidation. Methods in Enzymology 11 197–199CrossRefGoogle Scholar
Højrup, P. & Magnusson, S. 1987 Disulphide bridges of bovine Factor X. Biochemical Journal 245 887–891CrossRefGoogle ScholarPubMed
Hollar, C. M., Law, A. J. R., Dalgleish, D. G. & Brown, R. J. 1991 Separation of major casein fractions using cation-exchange fast protein liquid chromatography. Journal of Dairy Science 74 2403–2409CrossRefGoogle Scholar
Jollès, P., Alais, C. & Jollès, J. 1962 Amino acid composition of κ-casein and terminal amino acids of κ and para-κ-casein. Archives of Biochemistry and Biophysics 98 56–57CrossRefGoogle Scholar
Jones, W. K., Yu-Lee, L., Clift, S. M., Brown, T. L. & Rosen, J. M. 1985 The rat casein multigene family. Fine structure and evolution of the β-casein gene. Journal of Biological Chemistry 260 7042–7050CrossRefGoogle ScholarPubMed
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Mercier, J.-C. 1981 Phosphorylation of caseins. Present evidence for an amino acid triplet code post-translationally recognized by specific kinases. Biochimie 63 1–17CrossRefGoogle Scholar
Mercier, J.-C., Brignon, G. & Ribadeau-Dumas, B. 1973 [Primary structure of bovine κB-casein. Complete sequence.] European Journal of Biochemistry 35 222–235CrossRefGoogle ScholarPubMed
Nielsen, P. F., Klarskov, K., Højrup, P. & Roepstorff, P. 1988 Optimization of sample preparation for plasma desorption mass spectrometry of peptides and proteins using a nitrocellulose matrix. Biomedical and Environmental Mass Spectrometry 17 355–362CrossRefGoogle Scholar
Rasmussen, L. K., Højrup, P. & Petersen, T. E. 1992 a Localization of two interchain disulfide bridges in dimers of bovine αs2-casein. Parallel and antiparallel alignments of the polypeptide chains. European Journal of Biochemistry 203 381–386CrossRefGoogle Scholar
Rasmussen, L. K., Højrup, P. & Petersen, T. E. 1992 b The multimeric structure and the disulfide-bonding pattern of bovine κ-casein. European Journal of Biochemistry 207 215–222CrossRefGoogle ScholarPubMed
Rasmussen, L. K. & Petersen, T. E. 1991 Purification of disulphide-linked αs2- and κ-casein from bovine milk. Journal of Dairy Research 58 187–193CrossRefGoogle Scholar
Rollema, H. S., Brinkhuis, J. A. & Vreeman, H. J. 1988 1H-NMR studies of bovine κ-casein and casein micelles. Netherlands Milk and Dairy Journal 42 233–248Google Scholar
St-Martin, M. & Paquin, P. 1990 Ion-exchange fast protein liquid chromatography: optimization of the purification of caseins using a non-denaturing detergent. Journal of Dairy Research 57 63–68CrossRefGoogle Scholar
Snoeren, T. H. M., Van Markwijk, B. & Van Montfort, R. 1980 Some physicochemical properties of bovine αs2-casein. Biochimica el Biophysica Acta 622 268–276CrossRefGoogle Scholar
Sørensen, H. H., Thomsen, J. & Bayne, S. 1990 Strategies for determination of disulphide bridges in proteins using plasma desorption mass spectrometry. Biochemical and Environmental Mass Spectrometry 19 713–720CrossRefGoogle ScholarPubMed
Swaisgood, H. E., Brunner, J. R. & Lillevik, H. A. 1964 Physical parameters of κ-casein from cow's milk. Biochemistry 3 1616–1623CrossRefGoogle ScholarPubMed
Talbot, B. & Waugh, D. F. 1970 Micelle-forming characteristics of monomeric and covalent polymeric κ-caseins. Biochemistry 9 2807–2813CrossRefGoogle ScholarPubMed
Yu-Lee, L., Richter-Mann, L., Couch, C. H., Stewart, A. F., Mackinlay, A. G. & Rosen, J. M. 1986 Evolution of the casein multigene family: conserved sequences in the 5′ flanking and exon regions. Nucleic Acids Research 14 1883–1902CrossRefGoogle ScholarPubMed