Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-05T08:32:45.169Z Has data issue: false hasContentIssue false
  • English
  • Français

Dissemination of staphylococci in mice with experimental mastitis

Published online by Cambridge University Press:  01 June 2009

James C. Anderson
James C. Anderson
Affiliation:
AFRC Institute for Animal Diseases Research, Compton Laboratory, Newbury RG16 0NN, UK
Get access Rights & Permissions [Opens in a new window]

Summary

During the course of an acute, fatal mastitis in mice caused by intra-mammary injection of ∼ 105 cfu of Staphylococcus aureus, small numbers of staphylococci were detected in liver, spleen, kidney and heart. Transport from the mammary gland was by the venous blood system with no evidence of lymph node involvement. Intravenous injection of 100 times more staphylococci (∼107 cfu) than were fatal by the intramammary route failed to kill the mice and small numbers of staphylococci were found in the visceral organs. However, intravenous injection of ∼ 108 cfu of Staph. aureus was fatal and large numbers of staphylococci were found in the visceral organs. Treatment with cloxacillin (500 μg) by the intramammary or subcutaneous routes, or equally divided between these two routes, cleared the visceral organs of staphylococci; subcutaneous therapy had a limited and variable effect on numbers of staphylococci in the mammary gland while intramammary and divided treatments significantly reduced the numbers of staphylococci. Thus, staphylococci are disseminated to visceral organs during acute staphylococcal mastitis, but this makes a negligible contribution to the clinical disease.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 3 , August 1987 , pp. 339 - 345
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Anderson, J. C. 1976 The contribution of the mouse mastitis model to our understanding of staphylococcal infection. In Staphylococci and Staphylococcal Diseases (International Symposium 3, 1975) pp. 783–790 (Ed. Jeljaszewicz, J.) Stuttgart: Gustav Fischer Verlag (Zentralblatt für Bakteriologie, Parasitenkunde, Infektions-krankheiten und Hygiene, 1. Abteilung, Supplement 5)Google Scholar
Anderson, J. C. 1978 The effect of colonization of the mouse mammary gland by Staphylococcus epidermidis on subsequent infection with Staphylococcus aureus or Escherichia coli. Journal of Comparative Pathology 88 545–553CrossRefGoogle ScholarPubMed
Anderson, J. C. 1981 The behaviour of a capsulate strain of Staphylococcus aureus in the mammary gland of the mouse. In Staphylococci and Staphylococcus Infections (International Symposium 4,1979) pp. 667–672 (Ed. Jeljaszewicz, J.) Stuttgart: Gustav Fischer Verlag (Zentralblatt für Balcteriologie, Mikrobiologie und Hygiene, 1. Abteilung, Supplement 10)Google Scholar
Anderson, J. C. 1983 Veterinary aspects of staphylococci. In Staphylococci and Staphylococcal Infections vol. 1 pp. 193–241 (Ed. Easmon, C. S. F. and Adlam, C.) London: Academic PressGoogle Scholar
Anderson, J. C. & Craven, N. 1984 An assessment in the mouse of cefoperazone as a treatment for mastitis. Veterinary Record 114 607–612CrossRefGoogle ScholarPubMed
Gorrill, R. H. & McNeil, E. M. 1963 Staphylococcal infection in the mouse. I. The effect of route of injection. British Journal of Experimental Pathology 44 404–415Google ScholarPubMed
MacDiarmid, S. C. 1978 Antibacterial drugs used against mastitis in cattle by the systemic route. New Zealand Veterinary Journal 26 290–295CrossRefGoogle ScholarPubMed
Miles, A. A., Misra, S. S. & Irwin, J. O. 1938 The estimation of the bactericidal power of the blood. Journal of Hygiene 38 732–749Google ScholarPubMed
Moore, G. A. & Heider, L. E. 1984 Treatment of mastitis. Veterinary Clinics of North America 6 323–333Google ScholarPubMed
Schenkman, D. I., Berman, D. T. & Yandell, B. S. 1985 Effect of stage of lactation on transport of colloidal carbon or Staphylococcus aureus from the mammary gland lumen to lymph nodes in guinea pigs. Journal of Dairy Research 52 491–500CrossRefGoogle ScholarPubMed