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Differences in susceptibility to Mannheimia haemolytica-associated mastitis between two breeds of dairy sheep

Published online by Cambridge University Press:  24 April 2007

Ilectra A Fragkou ,
John Skoufos ,
Peter J Cripps ,
Ilias Kyriazakis ,
Nikos Papaioannou ,
Costas M Boscos ,
Athina Tzora  and
George C Fthenakis
Ilectra A Fragkou
Affiliation:
Veterinary Faculty, University of Thessaly, PO Box 199, 43100 Karditsa, Greece
John Skoufos
Affiliation:
Department of Animal Production, TEI Epirus, PO Box 110, 47100 Arta, Greece
Peter J Cripps
Affiliation:
Faculty of Veterinary Science, University of Liverpool, Neston, South Wirral, CH64 7TE, UK
Ilias Kyriazakis
Affiliation:
Veterinary Faculty, University of Thessaly, PO Box 199, 43100 Karditsa, Greece
Nikos Papaioannou
Affiliation:
School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Macedonia, Greece
Costas M Boscos
Affiliation:
School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Macedonia, Greece
Athina Tzora
Affiliation:
Department of Animal Production, TEI Epirus, PO Box 110, 47100 Arta, Greece
George C Fthenakis*
Affiliation:
Veterinary Faculty, University of Thessaly, PO Box 199, 43100 Karditsa, Greece
*
*For correspondence; e-mail: [email protected]
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Abstract

We used a Mannheimia haemolytica isolate to study differences in susceptibility to experimental mastitis between two breeds of dairy sheep. The isolate was deposited into the teat duct of Karagouniko (K, n=8) or Frisarta (F, n=8) ewes. The animals were monitored by means of clinical, bacteriological, cytological and pathological methods. K ewes did not develop any systemic or mammary clinical signs, whilst F ewes became ill and developed acute clinical mastitis 12 h later (P<0·001). Bacteria were isolated from 34/48 samples from K ewes and from 46/46 samples from F ewes. Positive California mastitis test (CMT) results were 17/24 samples from K ewes and 23/23 samples from F ewes; leucocytes were seen in Giemsa-stained films. Total pathology score summed over all group K ewes was 41 (maximum possible: 128); Man. haemolytica was isolated from 12/24 tissue samples. Total pathology score summed over all group F ewes was 93; Man. haemolytica was isolated from 24/24 tissue samples. Hyperplastic lymphoid nodules consisting of lymphocytes and plasma cells with germinal activity were characteristically present at the border between teat duct–teat cistern of group K ewes; no such structures were observed in teats of group F ewes. The results identified differences in susceptibility/resistance to a mastitis pathogen among animals of the two breeds. Defence mechanisms of the teat appeared to be inadequate against the invading organisms; as lymphoid nodules have been considered important defensive mechanisms of the ovine teat, their observed lack in Frisarta ewes might have predisposed them to development of mastitis.

Keywords

SheepmastitisMannheimia haemolyticabreed susceptibility
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 3 , August 2007 , pp. 349 - 355
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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References

Arriens, MA, Ruff, G, Schallibaum, M & Lazary, S 1994 Possible association between serologically detected haplotype of the bovine major histocompatibility complex and subclinical mastitis. Journal of Animal Breeding and Genetics 111 152–161CrossRefGoogle ScholarPubMed
Barillet, F, Rupp, R, Mignon-Grasteau, S, Astruc, JM & Jacquin, M 2001 Genetic analysis for mastitis resistance and milk somatic cell score in French Lacaune dairy sheep. Genetics Selection Evolution 33 397–415CrossRefGoogle ScholarPubMed
Baro, JA, Carriedo, JA & SanPrimitivo, F 1994 Genetic parameters of test day measures for somatic cell count, milk yield, and protein percentage of milking ewes. Journal of Dairy Science 77 2658–2662CrossRefGoogle ScholarPubMed
Barrow, GI & Feltham, RKA 1993 Manual for the Identification of Medical Bacteria, 3rd Edn. Cambridge, UK: Cambridge University PressCrossRefGoogle Scholar
Bergonier, D & Berthelot, X 2003 New advances in epizootiology and control of ewe mastitis. Livestock Production Science 79 1–16CrossRefGoogle Scholar
Bergonier, D, De Cremoux, R, Rupp, R, Lagriffoul, G, Berthelot, X 2003 Mastitis of dairy small ruminants. Veterinary Research 34 689–716CrossRefGoogle ScholarPubMed
Burriel, AR 1997 Evidence of breed susceptibility to experimentally produced ovine subclinical mastitis. Sheep and Goat Research Journal 13 20–23Google Scholar
Christodoulopoulos, G & Fthenakis, GC 2005 Respiratory infections in young lambs in Greece: status and field experience with their control. Proceedings of 6th International Sheep Veterinary Congress, Hersonissos, Greece, pp. 161–163Google Scholar
Detilleux, JC 2002 Genetic factors affecting susceptibility of dairy cows to udder pathogens. Veterinary Immunology and Immunopathology 88 103–110CrossRefGoogle ScholarPubMed
Detilleux, JC, Koehler, KJ, Freeman, AEF, Kehrli, ME Jr & Kelley, DH 1994 Immunological parameters of periparturient Holstein cattle, genetic variation. Journal of Dairy Science 77 2640–2645CrossRefGoogle ScholarPubMed
El-Masannat, ETS, Jones, JET & Scott, MJ 1991 The experimental production of mastitis in sheep by intramammary inoculation of Pasteurella haemolytica. Journal of Comparative Pathology 105 455–465CrossRefGoogle ScholarPubMed
Euzeby, JP 1997 List of bacterial names with standing in nomenclature: a folder available on the internet. International Journal of Systematic Bacteriology 47 590–592 (List of Prokaryotic Names with Standing in Nomenclature; last full update September 07, 2006. URL: http://www.bacterio.net)Google ScholarPubMed
Fragkou, IA, Mavrogianni, VS, Cripps, PJ, Gougoulis, DA & Fthenakis, GC 2007a The bacterial flora in the teat duct of ewes can prevent from and can cause mastitis. Veterinary Research (In Press)CrossRefGoogle Scholar
Fragkou, IA, Mavrogianni, VS, Papaioannou, N, Boscos, C, Cripps, PJ, Skoufos, J & Fthenakis, GC 2007b Presence of sub-epithelial lymphoid tissues in the teat of ewe-lambs and adult ewes. Small Ruminant Research doi: 0.1016/j.smallrumres.2006.05.003CrossRefGoogle Scholar
Fthenakis, GC 1994 Prevalence and aetiology of subclinical mastitis in ewes of Southern Greece. Small Ruminant Research 13 293–300CrossRefGoogle Scholar
Fthenakis, GC 1995 California Mastitis Test and Whiteside Test in diagnosis of subclinical mastitis of dairy ewes. Small Ruminant Research 16 271–276CrossRefGoogle Scholar
Fthenakis, GC & Jones, JET 1990a The effect of experimentally induced sublinical mastitis on milk yield of ewes and on the growth of lambs. British Veterinary Journal 146 43–49CrossRefGoogle Scholar
Fthenakis, GC & Jones, JET 1990b The effect of inoculation of coagulase-negative staphylococci into the ovine mammary gland. Journal of Comparative Pathology 102 211–219CrossRefGoogle ScholarPubMed
Gonzalez-Rodriguez, MC, Gonzalo, C, San Primitivo, F & Carmenes, P 1995 Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes. Journal of Dairy Science 78 2753–2759CrossRefGoogle ScholarPubMed
Kelm, SC, Detilleux, JC, Freeman, AE, Kehrli, ME Jr, Dietz, AB, Fox, LK, Butler, JE, Kasckovics, I & Kelley, DH 1997 Genetic association between parameters of innate immunity and measures of mastitis in periparturient Holstein cattle. Journal of Dairy Science 80 1767–1775CrossRefGoogle ScholarPubMed
Lydyard, PM & Rossi, CE 2001 Cells, tissues and organs of the immune system. In Immunology, 6th Edn, pp. 15–46 (Eds Roitt, I, Brostoff, J & Male, DK). Philadelphia PA, USA: MosbyGoogle Scholar
McShane, RD, Gallagher, DS, Newkirk, H, Taylor, JF, Burzlaff, JD, Davis, SK & Skow, LC 2001 Physical localization and order of genes in the class I region of the bovine MHC. Animal Genetics 32 235–239CrossRefGoogle Scholar
Mavrogianni, VS, Cripps, PJ, Brooks, H, Taitzoglou, IA & Fthenakis, GC 2007 Presence of sub-epithelial lymphoid nodules in the teat of ewes. Anatomia Histologia Embryologia doi: 10.1111/j.1439-0264.2006.00720.xCrossRefGoogle Scholar
Mavrogianni, VS, Cripps, PJ & Fthenakis, GC 2006a Description and validation of a novel technique to study the bacterial flora of the teat duct of ewes. Small Ruminant Research 66 258–264CrossRefGoogle Scholar
Mavrogianni, VS, Cripps, PJ, Papaioannou, N, Taitzoglou, I & Fthenakis, GC 2006b Teat disorders predispose ewes to mastitis after challenge with Mannheimia haemolytica. Veterinary Research 37 89–105CrossRefGoogle ScholarPubMed
Mavrogianni, VS, Fthenakis, GC, Brooks, H, Papaioannou, N, Cripps, PJ, Taitzoglou, I, Brellou, G & Saratsis, P 2005 The effects of inoculation of Mannheimia haemolytica into the teat of lactating ewes. Veterinary Research 36 13–25CrossRefGoogle ScholarPubMed
Miles, AA & Misra, JS 1938 The estimation of the bactericidal power of the blood. Journal of Hygiene, Cambridge 38 732–749Google ScholarPubMed
Moyron-Quiroz, JE, Rangel-Moreno, J, Kusser, K, Hartson, L, Sprague, F, Goodrich, S, Woodland, DL, Lund, FE & Randall, TD 2004 Role of inducible bronchus associated lymphoid tissue (iBALT) in respiratory immunity. Nature Medicine 10 927–934CrossRefGoogle ScholarPubMed
Saini, SS, Allore, B, Jacobs, RM & Kaushik, AT 1999 Exceptionally long CDR3H region with multiple cysteine residues in functional bovine IgM antibodies. European Journal of Immunology 29 2420–24263.0.CO;2-A>CrossRefGoogle ScholarPubMed
Saratsis, Ph, Alexopoulos, C, Tzora, A & Fthenakis, GC 1999 The effect of experimentally induced subclinical mastitis on the milk yield of dairy ewes. Small Ruminant Research 32 205–209CrossRefGoogle Scholar
Schalm, OW, Carroll, EJ & Jain, NC 1971 Bovine Mastitis. Philadelphia PA, USA: Lea and FebigerGoogle Scholar
Schukken, YH, Mallard, BA, Dekkers, JCM, Leslie, KE & Stear, MJ 1994 Genetic impact of intramammary infection following Staphylococcus aureus challenge. Journal of Dairy Science 77 639–647CrossRefGoogle ScholarPubMed
Schwerin, W, Solinas-Toldo, S, Eggen, A, Bruner, R, Seyfert, HM & Fries, R 1994 The bovine lactoferrin gene (LTF) maps to chromosome 22 and syntenic group U12. Mammalian Genome 5 486–489CrossRefGoogle ScholarPubMed
Shuster, DE, Kehrli, ME Jr, Rainard, P & Paape, M 1997 Complement fragment C5a and inflammatory cytokines in neutrophils recruitment during intramammary infection with Escherichia coli. Infection and Immunity 65 3286–3292CrossRefGoogle ScholarPubMed
Skoufos, J, Mavrogianni, VS, Tzora, A, Mavrommatis, I, Alexopoulos, C & Fthenakis, GC 2006 Use of lincomycin to control respiratory infections in lambs: effects on health and production. Small Ruminant Research 66 214–221CrossRefGoogle Scholar
Swiderek, WP, Charon, KM, Winnicka, A & Gruszczynska, J 2006 Relationship between blood lymphocyte phenotype, DRB1 (MHC class II) gene polymorphism and somatic cell count in ewe milk. Bulletin of the Veterinary Institute in Pulawy 50 73–77Google Scholar
Takeshima, SN & Aida, Y 2006 Structure, function and disease susceptibility of the bovine major histocompatibility complex. Animal Science Journal 77 138–150CrossRefGoogle Scholar
Watson, DL, Franklin, NA, Davies, HI, Kettlewell, P & Frost, AJ 1990 Survey of intramammary infections in ewes on the New England tableland of New South Wales. Australian Veterinary Journal 67 6–8CrossRefGoogle ScholarPubMed
Zygoyiannis, D 1999 Sheep Production. Thessaloniki, Greece: Sinchroni PediaGoogle Scholar
Zygoyiannis, D 2006 Sheep production in the world and in Greece. Small Ruminant Research 62 143–147CrossRefGoogle Scholar