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Development of an improved Streptococcus uberis experimental mastitis challenge model using different doses and strains in lactating dairy cows

Published online by Cambridge University Press:  20 July 2015

Manouchehr Khazandi ,
Patricia Eats ,
Darren Trott ,
Esmaeil Ebrahimie ,
Jeanette Perry ,
Elizabeth Hickey ,
Stephen Page ,
Sanjay Garg  and
Kiro R Petrovski
Manouchehr Khazandi
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Patricia Eats
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Darren Trott
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Esmaeil Ebrahimie
Affiliation:
Department of Genetics and Evolution, School of Biological Sciences, The University of Adelaide, South Australia, 5005, Australia Institute of Biotechnology, The University of Shiraz, Shiraz, Iran
Jeanette Perry
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Elizabeth Hickey
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
Stephen Page
Affiliation:
Luoda Pharma Pty Ltd, Caringbah, New South Wales 2229, Australia
Sanjay Garg
Affiliation:
School of Pharmacy and Medical Science, The University of South Australia, South Australia 5000, Australia
Kiro R Petrovski*
Affiliation:
School of Animal and Veterinary Sciences, The University of Adelaide, South Australia 5371, Australia
*
*For correspondence; e-mail: [email protected]
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Abstract

Developing a reliable mastitis challenge infection model is required to test new intramammary antimicrobial preparations, other novel bovine mastitis treatments, and study mastitis pathogenesis. Three treatment groups of Holstein Friesian cows in active lactation were administered two doses (104 and 106 cfu/quarter) on a single occasion with one of the three Streptococcus uberis strains (BFR6019, MFF1283 and SA002) suspended in 5 ml of sterile PBS, administered via intramammary inoculation immediately after milking. All quarters that were challenged with S. uberis strains MLF1283 and BFR6019 showed clinical signs of mastitis on day 1 and 2 after the challenge. Strain SA002 had a lower rate of inducing clinical mastitis which was detected later than day 3 after the challenge. We successfully developed a rapid and reliable model for inducing experimental S. uberis mastitis with 100% success rate in cows in active lactation. On the basis of the correlation results between strains, RAPD fingerprinting results, clinical findings, and a 100% success rate of mastitis induction for low and high doses S. uberis strains MLF1283 and BFR6019, strain virulence seems to be a more important effect than challenge dose in induction of clinical mastitis following experimental challenge.

Keywords

Mastitischallenge modeldairy cowsStreptococcus uberis
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 4 , November 2015 , pp. 470 - 477
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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References

Abureema, S, Smooker, P, Malmo, J & Deighton, M 2014 Molecular epidemiology of recurrent clinical mastitis due to Streptococcus uberis: evidence of both an environmental source and recurring infection with the same strain. Journal of Dairy Science 97 285–290CrossRefGoogle ScholarPubMed
Akers, RM & Nickerson, SC 2011 Mastitis and its impact on structure and function in the ruminant mammary gland. Journal of Mammary Gland Biology and Neoplasia 16 275–289CrossRefGoogle ScholarPubMed
Bannerman, DD, Paape, MJ, Goff, JP, Kimura, K, Lippolis, JD & Hope, JC 2004 Innate immune response to intramammary infection with Serratia marcescens and Streptococcus uberis. Veterinary Research 35 681–700CrossRefGoogle ScholarPubMed
Bennett, R, Christiansen, K & Clifton-Hadley, R 1999 Preliminary estimates of the direct costs associated with endemic diseases of livestock in Great Britain. Preventive Veterinary Medicine 39 155–171CrossRefGoogle ScholarPubMed
Halasa, T, Huijps, K, Osteras, O & Hogeveen, H 2007 Economic effects of bovine mastitis and mastitis management: a review. Veterinary Quarterly 29 18–31CrossRefGoogle ScholarPubMed
Harmon, RJ 1994 Physiology of mastitis and factors affecting somatic cell counts. Journal of Dairy Science 77 2103–2112CrossRefGoogle ScholarPubMed
Hendriksen, RS, Mevius, DJ, Schroeter, A, Teale, C, Meunier, D, Butaye, P, Franco, A, Utinane, A, Amado, A, Moreno, M, Greko, C, Stark, K, Berghold, C, Myllyniemi, AL, Wasyl, D, Sunde, M & Aarestrup, FM 2008 Prevalence of antimicrobial resistance among bacterial pathogens isolated from cattle in different European countries: 2002–2004. Acta Veterinaria Scandinavica 50 28CrossRefGoogle ScholarPubMed
Hogan, JS, Gonzales, RJ, Harmon, RJ, Nickerson, SC, Oliver, SP, Pankey, JW & Smith, KL 1999 Laboratory Handbook on Bovine Mastitis. Madison, WI, USA: National Mastitis Council IncWIGoogle Scholar
Jackson, KA, Nickerson, SC, Kautz, FM & Hurley, DJ 2012 Technical note: development of a challenge model for Streptococcus uberis mastitis in dairy heifers. Journal of Dairy Science 95 7210–7213CrossRefGoogle ScholarPubMed
Jayarao, BM, Gillespie, BE & Oliver, SP 1996 Application of randomly amplified polymorphic DNA fingerprinting for species identification of bacteria isolated from bovine milk. Journal of Food Protection 59 615–620CrossRefGoogle ScholarPubMed
Leigh, JA 1999 Streptococcus uberis: a permanent barrier to the control of bovine mastitis? Veterinary Journal 157 225–238CrossRefGoogle Scholar
Lopez-Benavides, MG, Williamson, JH, Pullinger, GD, Lacy-Hulbert, SJ, Cursons, RT & Leigh, JA 2007 Field observations on the variation of Streptococcus uberis populations in a pasture-based dairy farm. Journal of Dairy Science 90 5558–5566CrossRefGoogle Scholar
McDougall, S, Parker, K, Swift, S, Harcourt, S & Sutherland, G 2004 Effect of dose of Streptococcus uberis infused into the mammary gland of lactating cows on clinical signs, bacterial count, somatic cell count and milk production. Proceedings of the New Zealand Society of Animal Science 64 143–146Google Scholar
Milinovich, GJ, Trott, DJ, Burrell, PC, van Eps, AW, Thoefner, MB, Blackall, LL, Al Jassim, RA, Morton, JM & Pollitt, CC 2006 Changes in equine hindgut bacterial populations during oligofructose-induced laminitis. Environmental Microbiology 8 885–898CrossRefGoogle ScholarPubMed
Milner, P, Page, KL & Hillerton, JE 1997 The effects of early antibiotic treatment following diagnosis of mastitis detected by a change in the electrical conductivity of milk. Journal of Dairy Science 80 859–863CrossRefGoogle ScholarPubMed
Nickerson, SC, Boddie, RL, Owens, WE & Watts, JL 1990 Effects of novel intramammary device models on incidence of mastitis after experimental challenge. Journal of Dairy Science 73 2774–2784CrossRefGoogle ScholarPubMed
Paape, MJ, Schultze, WD, Corlett, NJ & Weinland, BT 1988 Effect of abraded intramammary device on outcome in lactating cows after challenge exposure with Streptococcus uberis. American Journal of Veterinary Research 49 790–792Google ScholarPubMed
Petrovski, KR, Trajcev, M & Buneski, G 2006 A review of the factors affecting the costs of bovine mastitis. Journal of the South African Veterinary Association 77 52–60CrossRefGoogle ScholarPubMed
Petrovski, KR, Heuer, C, Parkinson, TJ & Williamson, NB 2009 The incidence and aetiology of clinical bovine mastitis on 14 farms in Northland, New Zealand. New Zealand Veterinary Journal 57 109–115CrossRefGoogle ScholarPubMed
Petrovski, KR, Caicedo-Caldas, A, Williamson, NB, Lopez-Villalobos, N, Grinberg, A, Parkinson, TJ & Tucker, IG 2011a Efficacy of a novel internal dry period teat sealant containing 0·5% chlorhexidine against experimental challenge with Streptococcus uberis in dairy cattle. Journal of Dairy Science 94 3366–3375CrossRefGoogle ScholarPubMed
Petrovski, KR, Williamson, NB, Lopez-Villalobos, N, Parkinson, TJ & Tucker, IG 2011b Culture results from milk samples submitted to veterinary diagnostic laboratories from August 2003 to December 2006 in New Zealand. New Zealand Veterinary Journal 59 317–322CrossRefGoogle ScholarPubMed
Phuektes, P, Mansell, PD, Dyson, RS, Hooper, ND, Dick, JS & Browning, GF 2001 Molecular epidemiology of Streptococcus uberis isolates from dairy cows with mastitis. Journal of Clinical Microbiology 39 1460–1466CrossRefGoogle ScholarPubMed
Reinoso, EB, Lasagno, MC, Dieser, SA & Odierno, LM 2011 Distribution of virulence-associated genes in Streptococcus uberis isolated from bovine mastitis. FEMS Microbiology Letters 318 183–188CrossRefGoogle ScholarPubMed
Sharma, N, Singh, NK & Bhadwal, MS 2011 Relationship of somatic cell count and mastitis: an overview. Asian-Australasian Journal of Animal Sciences 24, 429–438CrossRefGoogle Scholar
Tamilselvam, B, Almeida, RA, Dunlap, JR & Oliver, SP 2006 Streptococcus uberis internalizes and persists in bovine mammary epithelial cells. Microbial Pathogenesis 40 279–285CrossRefGoogle ScholarPubMed
Tiwari, JG, Babra, C, Tiwari, H, Williams, V, De Wet, S, Gibson, J, Paxman, A, Morgan, E, Costantino, P, Sunagar, R, Isloor, S & Mukkur, T 2013 Trends in therapeutic and prevention strategies for management of bovine mastitis: an overview. Journal of Vaccines and Vaccination 4 1–11CrossRefGoogle Scholar
Wang, L, Chen, W, Zhang, L & Zhu, Y 2013 Genetic diversity of Streptococcus uberis isolates from dairy cows with subclinical mastitis in Southern Xinjiang Province, China. Journal of General and Applied Microbiology 59 287–293CrossRefGoogle ScholarPubMed
Wang, W, Song, Y, Petrovski, K, Eats, P, Trott, DJ, Wong, HS, Page, SW, Perry, J & Garg, S 2015 Development of intramammary delivery systems containing lasalocid for the treatment of bovine mastitis: impact of solubility improvement on safety, efficacy, and milk distribution in dairy cattle. Journal of Drug Design, Development and Therapy 9 631–642Google ScholarPubMed
Watts, JL, Papich, MG, Bade, DJ, Brown, SD, Fajt, VR, Fritsche, TR, Heine, HS, Hunter, RP, Schwartz, S, Silley, P, Wu, CC & Zurenko, GE 2013 Performance standard for antimicrobial disk and dilution susceptibility testing for bacteria isolated from animals. In Approved Standard, 4th edition., Vol. VET-01-A4. CLSI document M31-A4. Wayne, PA, USA: Clinical and Laboratory Standards InstituteGoogle Scholar
Zadoks, RN, Gillespie, BE, Barkema, HW, Sampimon, OC, Oliver, SP & Schukken, YH 2003 Clinical, epidemiological and molecular characteristics of Streptococcus uberis infections in dairy herds. Epidemiology and Infection 130 335–349CrossRefGoogle ScholarPubMed