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Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism

Published online by Cambridge University Press:  06 August 2001

CÉLIA MAUDET  and
PIERRE TABERLET
CÉLIA MAUDET
Affiliation:
Laboratoire de Biologie des Populations d'Altitude, CNRS UMR5553, Université Joseph Fourier, BP53, F-38041 Grenoble, Cedex 9 France
PIERRE TABERLET
Affiliation:
Laboratoire de Biologie des Populations d'Altitude, CNRS UMR5553, Université Joseph Fourier, BP53, F-38041 Grenoble, Cedex 9 France
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Abstract

A new polymerase chain reaction (PCR)-based method was developed to detect cows' milk in goat cheese. This method is based on mitochondrial DNA (mtDNA) control region sequence variations. DNA extractions from 150 mg of cheese were carried out using a spin column-based method. Subsequent PCR amplifications of DNA were performed with cow specific primers, demonstrating the ability to detect cows' milk in a variety of cheeses. This simple approach provides high quality DNA, and is shown to be very sensitive, with a detection limit of less than 0·1% of cows' milk. Analysis of an agarose gel digital image allows a rough estimation of the percentage of cows' milk used in adulteration.

Keywords

Goats' milkcheesecows' milkdetectionPCRmitochondrial DNA
Type
Original article
Information
Journal of Dairy Research , Volume 68 , Issue 2 , May 2001 , pp. 229 - 235
Copyright
Proprietors of Journal of Dairy Research 2001

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