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Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil

Published online by Cambridge University Press:  14 May 2020

Nathália Brancato Junqueira ,
Anelise Salina ,
Gabriela Capriogli Oliveira ,
Elena Mettifogo ,
Sâmea Fernandes Joaquim ,
Felipe Freitas Guimarães ,
Felipe Morales Dalanezi  and
Helio Langoni
Nathália Brancato Junqueira
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
Anelise Salina
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
Gabriela Capriogli Oliveira
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
Elena Mettifogo
Affiliation:
Laboratti Veterinary Diagnostic Laboratory, Pedrinhas Paulista, São Paulo State, Brazil
Sâmea Fernandes Joaquim
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
Felipe Freitas Guimarães
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
Felipe Morales Dalanezi
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
Helio Langoni*
Affiliation:
Department of Veterinary Hygiene and Public Health, São Paulo State University (Unesp), School of Veterinary Medicine and Animal Science, Botucatu, São Paulo State, Brazil
*
Author for correspondence: Helio Langoni, Email: [email protected]
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Abstract

The work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence.

Keywords

Mastitismicrobiologymolecular biologyMycoplasma bovisMycoplasmosis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 3 , August 2020 , pp. 306 - 308
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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