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Detection of Brucella spp. in soft cheese by semi-nested polymerase chain reaction

Published online by Cambridge University Press:  09 May 2003

Giuseppina M Tantillo ,
Angela Di Pinto  and
Canio Buonavoglia
Giuseppina M Tantillo
Affiliation:
Dipartimento di Sanità e Benessere degli Animali – Università degli Studi – Bari, Provinciale per Casamassima, km 3, 70010 Valenzano – Bari, Italy
Angela Di Pinto
Affiliation:
Dipartimento di Sanità e Benessere degli Animali – Università degli Studi – Bari, Provinciale per Casamassima, km 3, 70010 Valenzano – Bari, Italy
Canio Buonavoglia
Affiliation:
Dipartimento di Sanità e Benessere degli Animali – Università degli Studi – Bari, Provinciale per Casamassima, km 3, 70010 Valenzano – Bari, Italy
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Abstract

Brucellae are Gram-negative bacteria with a predilection for the reticuloendothelial system and the reproductive tract of cattle, sheep, goats and pigs. In these species brucellosis is of great economic significance because of the resulting abortion and infertility (Samartino et al. 1993; Corbel, 1997; Altecruse et al. 1998). Human brucellosis, caused by contamination from infected animals (percutaneous, conjunctival or nasal infection), is very common and remains a serious health hazard in Mediterranean Europe, western Asia, and parts of Africa and South America (Boschiroli et al. 2001). Raw milk, butter, ice-cream and unpasteurized cheese are also potential vehicles for the transmission of Brucella.

Keywords

Brucella spp.soft cheesessemi-nested PCR
Type
Brief Report
Information
Journal of Dairy Research , Volume 70 , Issue 2 , May 2003 , pp. 245 - 247
Copyright
Proprietors of Journal of Dairy Research 2003

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