Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-28T05:44:33.217Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of 70 kDa heat shock protein in the saliva of dairy cows

Published online by Cambridge University Press:  19 June 2017

Elsa Lamy ,
Viktor Jurkovich ,
Lénia Rodrigues ,
Ana Geraldo ,
Liliana Cachucho ,
Flávio Silva ,
Catarina Matos ,
Fernando Capela e Silva ,
Cristina Pinheiro  and
László Könyves
...Show all authors
Elsa Lamy
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal
Viktor Jurkovich*
Affiliation:
Department of Animal Hygiene, Herd Health and Veterinary Ethology, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary
Lénia Rodrigues
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal
Ana Geraldo
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal
Liliana Cachucho
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal
Flávio Silva
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal
Catarina Matos
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal
Fernando Capela e Silva
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal Department of Biology, University of Evora, Largo dos Colegiais 2, 7000 Évora, Portugal
Cristina Pinheiro
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal Department of Zootechnics, University of Evora, Largo dos Colegiais 2, 7000 Évora, Portugal
László Könyves
Affiliation:
Department of Animal Hygiene, Herd Health and Veterinary Ethology, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary
Mikolt Bakony
Affiliation:
Department of Animal Hygiene, Herd Health and Veterinary Ethology, University of Veterinary Medicine, István utca 2, H-1078 Budapest, Hungary
Alfredo Pereira
Affiliation:
Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94 7006-554, Portugal Department of Zootechnics, University of Evora, Largo dos Colegiais 2, 7000 Évora, Portugal
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

This Research Communication describes, for the first time, the detection of HSP70 in saliva of dairy cows. Thermal stress is a major environmental stress that limits animal growth, metabolism, and productivity. The cellular response to heat stress involves the synthesis of heat shock proteins (HSPs), presumably to protect the functional stability of cells at increasing temperatures. HSP70 has been found to be present in cattle blood serum and may also be present in other secretory fluids, such as saliva, as already observed in humans. The aim of this study was to detect heat shock protein HSP70 in bovine saliva. Saliva samples were taken from higher- (n = 5) and lower milk producing (n = 5) Holstein-Friesian cows in summer and in winter for the detection of HSP70. HSP70 concentrations were assayed using the ELISA technique. Salivary HSP70 concentrations ranged from 0·524 to 12·174 ng/ml in cows. Higher salivary HSP70 concentrations were significantly associated with higher milk production and higher environmental temperature, but not with rectal temperature.

Keywords

heat shock proteinsdairy cowsaliva
Type
Research Article
Information
Journal of Dairy Research , Volume 84 , Issue 3 , August 2017 , pp. 280 - 282
Copyright
Copyright © Proprietors of Journal of Dairy Research 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

The authors contributed equally.

References

Allen, JD, Hall, LW, Collier, RJ & Smith, JF 2015 Effect of core body temperature, time of day, and climate conditions on behavioural patterns of lactating dairy cows experiencing mild to moderate heat stress. Journal of Dairy Science 98 118–127 Google Scholar
Banerjee, D, Upadhyay, RC, Chaudhary, UB, Kumar, R, Singh, S, Ashutosh, M., Polley, S, Mukherjee, A, Das, TK & De, S 2014 Seasonal variation in expression pattern of genes under HSP70 family in heat- and cold-adapted goats (Capra hircus). Cell Stress Chaperones 19 401–408 Google Scholar
Collier, RJ, Dahl, GE & VanBaale, MJ 2006 Major advances associated with environmental effects on dairy cattle. Journal of Dairy Science 89 1244–1253 CrossRefGoogle ScholarPubMed
Daugaard, M, Rohde, M & Jäättelä, M 2007 The heat shock protein 70 family: highly homogenous proteins with overlapping and distinct functions. FEBS Letters 581 3702–3710 Google Scholar
Fábián, TK, Gáspár, J, Fejérdy, L, Kaán, B, Bálint, M, Csermely, P & Fejérdy, P 2003 Hsp70 is present in human saliva. Medical Science Monitor 9 BR62–BR65 Google Scholar
Gaughan, JB, Bonner, SL, Loxton, I & Mader, TL 2013 Effects of chronic heat stress on plasma concentration of secreted heat shock protein 70 in growing feedlot cattle. Journal of Animal Science 91 120–129 CrossRefGoogle ScholarPubMed
Ireland, HE, Leoni, F, Altaie, O, Birch, CS, Coleman, RC, Hunter-Lavin, C & Williams, JH 2007 Measuring the secretion of heat shock proteins from cells. Methods 2007 43 176–183 CrossRefGoogle Scholar
Kregel, KC 2002 Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. Journal of Applied Physiology 5 2177–2186 CrossRefGoogle Scholar
Lamy, E & Mau, M 2012 Saliva proteomics as an emerging, non-invasive tool to study livestock physiology, nutrition and diseases. Journal of Proteomics 75 4251–4258 Google Scholar
Slimen, B, Najar, T, Ghram, A & Abdrranna, M 2016 Heat stress effects on livestock: molecular, cellular and metabolic aspects, a review. Journal of Animal Physiology and Animal Nutrition 100 401–412 CrossRefGoogle Scholar
West, JW 2003 Effects of heat-stress on production in dairy cattle. Journal of Dairy Science 86 2131–2144 CrossRefGoogle ScholarPubMed
Yániz, JL, López-Gatius, L, Almeri, S, Carretero, T, Garca-Ispierto, I, Serrano, B, Smith, RF, Dobson, H & Santolaria, P 2009 Dynamics of heat shock protein 70 concentrations in peripheral blood lymphocyte lysates during pregnancy in lactating Holstein-Friesian cows. Theriogenology 72 1041–1046 CrossRefGoogle ScholarPubMed