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Crude goat whey fermentation by Kluyveromyces marxianus and Lactobacillus rhamnosus: contribution to proteolysis and ACE inhibitory activity

Published online by Cambridge University Press:  05 January 2009

Vanessa Hamme ,
Frederic Sannier ,
Jean-Marie Piot ,
Sandrine Didelot  and
Stephanie Bordenave-Juchereau
Vanessa Hamme
Affiliation:
Laboratoire LIENS, CNRS UMR 6250, site Marie Curie, UFR Sciences, Avenue Michel Crépeau, F-17042 La Rochelle cedex 01, France
Frederic Sannier
Affiliation:
Laboratoire LIENS, CNRS UMR 6250, site Marie Curie, UFR Sciences, Avenue Michel Crépeau, F-17042 La Rochelle cedex 01, France
Jean-Marie Piot
Affiliation:
Laboratoire LIENS, CNRS UMR 6250, site Marie Curie, UFR Sciences, Avenue Michel Crépeau, F-17042 La Rochelle cedex 01, France
Sandrine Didelot
Affiliation:
Laboratoire LIENS, CNRS UMR 6250, site Marie Curie, UFR Sciences, Avenue Michel Crépeau, F-17042 La Rochelle cedex 01, France
Stephanie Bordenave-Juchereau*
Affiliation:
Laboratoire LIENS, CNRS UMR 6250, site Marie Curie, UFR Sciences, Avenue Michel Crépeau, F-17042 La Rochelle cedex 01, France
*
*For correspondence; e-mail: [email protected]
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Abstract

Unsupplemented acid goat whey containing 0·96% protein and 2·76% lactose was fermented aerobically with 32 microflora extracted from various raw milk cheeses and dairy products. These microflora were screened for their ability to hydrolyse whey proteins (α-lactalbumin and/or β-lactoglobulin) and to generate peptides inhibitors of Angiotensin I Converting Enzyme. Five microflora were able to degrade whey protein. The most efficient microflora was able to fully hydrolyse α-lactalbumin and to a lesser extend β-lactoglobulin. It was extracted from Bamalou des Pyrenées cheese. Micro-organisms involved consisted of yeast Kluyveromyces marxianus and lactobacillus Lactobacillus rhamnosus. Both were able to produce ACE inhibitory peptides after whey fermentation.

Keywords

Whey fermentationKluyveromyces marxianusLactobacillus rhamnosusACE inhibition
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 152 - 157
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

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References

Belem, MAF, Gibbs, BF & Lee, BH 1999 Proposing sequence for peptides derived from whey fermentation with potential bioactive sites. Journal of Dairy Science 82 486–493CrossRefGoogle ScholarPubMed
Bintsis, T, Vafopoulou-Mastrojiannaki, A, Litopoulou-Tzanetaki, E & Robinson, RK 2003 Protease, peptidase and esterase activities by lactobacilli and yeast isolates from Feta cheese brine. Journal of Applied Microbiology 95 68–77CrossRefGoogle ScholarPubMed
Blendford, DE 1996 Whey from waste to gold. International Food Ingredients 1 27–29Google Scholar
Crow, V, Curry, B & Hayes, M 2001 The ecology of non-starter lactic acid bacteria (NSLAB) and their use as adjuncts in New Zealand Cheddar. International Dairy Journal 11 275–283CrossRefGoogle Scholar
Didelot, S, Bordenave-Juchereau, S, Rosenfeld, E, Piot, JM & Sannier, F 2006a Peptides released from acid goat whey by a yeast-lactobacillus association isolated from cheese microflora. Journal of Dairy Research 73 163–170CrossRefGoogle ScholarPubMed
Didelot, S, Bordenave-Juchereau, S, Rosenfeld, E, Fruitier-Arnaudin, I, Piot, JM & Sannier, F 2006b Preparation of angiotensin-I-converting enzyme inhibitory hydrolysates from unsupplemented caprine whey fermentation by various cheese microflora. International Dairy Journal 16 976–983CrossRefGoogle Scholar
Dziekak, JD 1987 Yeast and Yeast derivatives, definitions, characteristics and processing. Food Technoogy 41 104–112Google Scholar
Ferreira, IMPLVO, Pino, O, Mota, MV, Tavares, P, Pereira, A, Gonçalves, MP, Torres, D, Rocha, C & Teixeira, JA 2007 Preparation of ingredients containing an ACE-inhibitory peptide by tryptic hydrolysis of whey protein concentrates. International Dairy Journal 17 481–487CrossRefGoogle Scholar
Gobbetti, M, Ferranti, P, Smacchi, E, Goffredi, F & Addeo, F 2000 Production of angiotensin-I-converting enzyme inhibitory peptides in fermented milks started by Lactobacilllus delbrueckii subsp. bulgaricus SS1 and Lactococcus lactis subsp. cremoris FT4. Applied and Environmental Microbiology 66 3898–3904CrossRefGoogle Scholar
Gonzalez-Siso, MI 1996 The biotechnological utilization of cheese whey: a review. Bioresource Technology 57 1–11CrossRefGoogle Scholar
Hernández-Ledesma, B, Recio, I, Ramos, M & Amigo, L 2002 Preparation of ovine and caprine β-lactoglobulin hydrolysates with ACE-inhibitory activity. Identification of active peptides from caprine β-lactoglobulin hydrolysed with thermolysin. International Dairy Journal 12 805–812CrossRefGoogle Scholar
Holmquist, B, Bunning, P & Riordan, JF 1979 A continuous spectrophotometric assay for angiotensin converting enzyme. Analytical Biochemistry 95 540–548CrossRefGoogle ScholarPubMed
Ichaurrondo, VA, Yantorno, OM & Voget, CE 1993 Yeast growth and β-galactosidase production during aerobic batch cultures in lactose-limited synthethic medium. Process Biochemistry 29 47–54CrossRefGoogle Scholar
Jauhiainen, T, Colline, M, Narva, M, Cheng, ZJ, Poussa, T, Vapaatalo, H & Korpela, R 2005 Effect of long-term intake of milk peptides and minerals on blood pressure and arterial function in spontaneously hypertensive rats. Milchwissenschaft 60 358–363Google Scholar
Jimenez-Colmenero, F, Carballo, J & Cofrades, S 2001 Healthier meat and meat products: their role as fonctional foods. Meat Science 59 5–13CrossRefGoogle Scholar
Klein, N, Zourari, A & Lortal, S 2002 peptidase activity of four yeast species frequently encountered in dairy products-comparison with several dairy bacteria. International Dairy Journal 12 853–861CrossRefGoogle Scholar
Leclerc, PL, Gauthier, SF, Bachelard, H, Santure, M & Roy, D 2002 Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus. International Dairy Journal 12 995–1004CrossRefGoogle Scholar
Lopez-Fandiño, R, Otte, J & Van Camp, J 2006 Physiological, chemical and technological aspects of milk-protein-derived peptides with antihypertensive and ACE-inhibitory activity. International Dairy Journal 16 1277–1293CrossRefGoogle Scholar
Loureiro, V & Querol, A 1999 The prevalence and control of spoilage yeasts in foods and beverages. Trends in Food Science and Technology 10 356–365CrossRefGoogle Scholar
Lourenço da Costa, E, da Rocha Gontijo, JA & Netto, FM 2007 Effect of heat and enzymatic treatment on the antihypertensive activity of whey protein hydrolysates. International Dairy Journal 17 632–640CrossRefGoogle Scholar
Mullaly, MM, Meisel, H & FitzGerald, RJ 1997a Angiotensin-I-converting enzyme inhibitory activities of gastric and pancreatic proteinase digests of whey proteins. International Dairy Journal 7 299–303CrossRefGoogle Scholar
Nguyen, HV, Pulvirenti, A & Gaillardin, C 2000 Rapid differentiation of the closely related Kluyveromyces lactis var. lactis and Kluyveromyces marxianus strains isolated from dairy products using selective media and PCR/RFLP of the rDNA non transcribed spacer 2. Canadian Journal of Microbiology 46 1115–1122CrossRefGoogle Scholar
Otte, J, Shalaby, SMA, Zakora, M & Nielsen, MS 2007 Fractionation and identification of ACE-inhibitory peptides from α-lactalbumin and β-casein produced by thermolysin-catalysed hydrolysis. International Dairy Journal 17 1460–1472CrossRefGoogle Scholar
Pitt, JL & Hocking, AD 1999 Fungi and food spoilage. New York: Chapman & HallGoogle Scholar
Plessas, S, Bosnea, L, Psarianos, C, Koutinas, AA, Marchant, R & Banat, IM 2007 Lactic acid production by mixed cultures of Kluyveromyces marxianus, Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus helveticus. Bioresource Technology 99 5951–5955CrossRefGoogle ScholarPubMed
Reddy, IM, Kella, NKD & Kinsella, JE 1988 Structural and conformational basis of the resistance of β-lactoglobulin to peptic and chymotryptic digestion. Journal of Agricultural and Food Chemistry 36 737–741CrossRefGoogle Scholar
Salminen, S, Isolauri, E & Salminen, E 1996 Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology 70 347–358CrossRefGoogle ScholarPubMed
Sannier, F, Bordenave, S & Piot, JM 2000 Purification of goat beta-lactoglobulin from whey by an ultrafiltration membrane enzymic reactor. Journal of Dairy Research 67 43–51CrossRefGoogle ScholarPubMed
Saxelin, M 1997 Lactobacillus GG-a human probiotic strain with thorough clinical documentation. Food Reviews International 13 293–313CrossRefGoogle Scholar
Schmidt, DG & Poll, JK 1991 Enzymatic hydrolysis of whey proteins. Hydrolysis of α-lactalbumin and β-lactoglobulin in buffer solutions by proteolytic enzymes. Netherlands Milk and Dairy Journal 45 225–240Google Scholar
Tuomilehto, J, Lindström, J, Hyrynen, J, Korpela, R, Karhunen, ML, Mikola, L, Jauhiainen, T, Seppo, L & Nissinen, A 2004 Effect of ingesting sour milk fermented using Lactobacillus helveticus bacteria producing tripeptides on blood pressure in subjects with mild hypertension. Journal of Human Hypertension 18 795–802CrossRefGoogle ScholarPubMed
Urbina, EC, Munoz, ARN, Rojas, FJM, Ramirez, CJ, Ordaz, NR & Mayer, JG 2000 Batch and fed-batch cultures for the treatment of whey with mixed yeast cultures. Process Biochemistry 25 657–694Google Scholar
Vermeirssen, V, Van Camp, J, Decroos, K, Van Wijmelbeke, L & Verstraete, W 2003 The impact of fermentation and in vitro digestion on the formation of angiotensin-I-converting enzyme inhibitory activity from pea and whey protein. Journal of Dairy Science 86 429–438CrossRefGoogle ScholarPubMed
Viljoen, BC 2001 The interaction between yeast and bacteria in dairy environments. International Journal of Food Microbiology 69 37–44CrossRefGoogle ScholarPubMed
Yamamoto, N, Maeno, M & Takano, T 1999 Purification and characterization of an antihypertensive peptide from a yogurt-like product fermented by Lactobacillus helveticus CPN4. Journal of Dairy Science 82 1388–1393CrossRefGoogle ScholarPubMed