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Correlation between mastitis occurrence and the count of microorganisms in bulk raw milk of bovine dairy herds in four selective culture media

Published online by Cambridge University Press:  26 November 2009

Luís I M Souto ,
Clarice Y Minagawa ,
Evelise O Telles ,
Márcio A Garbuglio ,
Marcos Amaku ,
Priscilla A Melville ,
Ricardo A Dias ,
Sonia T Sakata  and
Nilson R Benites
Luís I M Souto*
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Clarice Y Minagawa
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Evelise O Telles
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Márcio A Garbuglio
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Marcos Amaku
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Priscilla A Melville
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Ricardo A Dias
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Sonia T Sakata
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
Nilson R Benites
Affiliation:
University of São Paulo, Faculty of Veterinary Medicine, Department of Preventive Veterinary and Animal Health, Av. Prof. Dr. Orlando Marques de Paiva, 87 – CEP 05508-900, São Paulo, Brazil
*
*For correspondence; e-mail: [email protected]
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Abstract

Milk is the normal secretion of the mammary gland, practically free of colostrum and obtained by the complete milking of one or more healthy animals. Mastitis is an inflammatory process of the mammary gland and it may cause alterations in the milk. The present work aimed to verify whether it is possible, by means of the counts of microorganism in the bulk raw milk in four selective culture media, to establish a correlation with the occurrence of mastitis and therefore, to monitor this disease in bovine dairy herds. The following selective culture media were used: KF Streptococcus Agar, Edwards Agar, Baird-Parker Agar, Blood Agar plus potassium tellurite. Spearman's correlation coefficient was calculated in order to compare the occurrence of mastitis (percentage) in each herd with respective selective culture media counts of microorganisms in bulk raw milk. Thirty-six possibilities were analysed (Tamis and CMT-positive rates were compared with the log-transformed count in four selective culture media) and there was a negative correlation between Tamis 3 and the Baird-Parker Agar plate count. The total results of microbiological tests showed that there were three correlations of the counts in selective culture media. Fifty-two possibilities were analysed and there was a negative correlation between no-bacterial-growth mastitis rates and log10 of KF Streptoccocus Agar plate count and there were two positive correlations between coagulase-positive staphylococci and log10 of Baird-Parker Agar plate count and Blood Agar plus potassium tellurite plate count.

Keywords

Mastitisbulk raw milkselective culture media
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 1 , February 2010 , pp. 63 - 70
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

American Public Health Association (APHA) 2001 Compendium of Methods for the Microbiological Examination of Foods 4th Edn, p. 676. Washington DC, USA: APHAGoogle Scholar
Audist, MJ, Coats, S, Sutherland, BJ, Mayes, JJ, McDowell, GH & Rogers, GL 1996 Effects of somatic cell count and stage of lactation on raw milk composition and the yield and quality of Cheddar cheese. Journal of Dairy Research 63 269–280CrossRefGoogle Scholar
Bennett, RM, Christiansen, K & Clifton-Handley, RS 1999 Estimating the costs associated with endemic diseases of dairy cattle. Journal of Dairy Research 66 455–459CrossRefGoogle ScholarPubMed
Blood, DC & Radostits, OM 1991 Veterinary Medicine 7th Edn, pp. 501–559. London, UK: Baillière TindallGoogle Scholar
Brazil 2002 Ministry of Agriculture, Livestock and Supply. Normative Instruction No. 51, September 20th 2002. Approving the Technical Regulations for the Production, Identity and Quality of Milk Type A, Type B, Type C, Pasteurized Milk and Refrigerated Raw Milk and the Technical Regulation for the Collection of Refrigerated Raw Milk and its In Bulk Transportation. Union Official Diary, Brasília, p. 13, 21 September 2002, Section 1Google Scholar
Cooney, S, Tiernan, D, Joyce, P & Kelly, AL 2000 Effect of somatic cell count and polymorphonuclear leucocyte content of milk on composition and proteolysis during ripening of Swiss-type cheese. Journal of Dairy Research 67 301–307CrossRefGoogle ScholarPubMed
Costa, EO, Coutinho, SD, Castilho, W, Teixeira, CM, Gambale, W, Gandra, CR & Pires, MF 1986 Bacterial aetiology of bovine mastitis, São Paulo State, Brazil. Brazilian Journal of Microbiology 17 107–112Google Scholar
Cullor, JS & Tyler, JW 1996 Mammary gland health and disorders. In: Large Animal Internal Medicine: Disease of Horses, Cattle, Sheep and Goats, 2nd Ednpp. 1177–1197 (Ed Smith, BP) St Louis MO, USA: Mosby-Year BookGoogle Scholar
Daniel, RCW, Biggs, DA & Barnum, DA 1966 The relationship between California Mastitis Test scores and monthly milk production and composition. Canadian Veterinary Journal 7 99–105Google ScholarPubMed
EU 2004 Corrigendum to Regulation (EC) No 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for food of animal origin. Official Journal of the European Union, april 29 of 2004. Available: <http://eur-lex.europa.eu/LexUriServ/LexUriServ.do?uri=OJ:L:2004:226:0022:0082:EN:PDF> Access: 02/21/2008+Access:+02/21/2008>Google Scholar
Ferguson, JD, Azzaro, G, Gambina, M & Licitra, G 2007 Prevalence of mastitis pathogens in Ragusa, Sicily, from 2000 to 2006. Journal of Dairy Science 90 5798–5813CrossRefGoogle ScholarPubMed
Fernandes, AM, Oliveira, CAF & Lima, CG 2007 Effects of somatic cell counts in milk on physical and chemical characteristics of yogurt. International Dairy Journal 17 111–115CrossRefGoogle Scholar
Fitz-Gerald, CH, Deeth, HC & Kitchen, BJ 1981 The relationship between the levels of free fatty acids, lipoprotein lipase, carboxilesterase, N-acetyl-β-D-glucosaminidase, somatic cell count and other mastitis indices in bovine milk. Journal of Dairy Research 48 253–265CrossRefGoogle Scholar
Haenlein, GFW, Schultz, LH & Zikakis, JP 1973 Composition of proteins in milk with varying leucocytes contents. Journal of Dairy Science 56 1017–1024CrossRefGoogle ScholarPubMed
Haas, Y, Veerkamp, RF, Barkema, HW, Gröhn, YT & Schukken, YH 2004 Association between pathogen-specific cases of clinical mastitis and somatic cell count patterns. Journal of Dairy Science 87 95–105CrossRefGoogle ScholarPubMed
Hortet, P & Seegers, H 1998 Loss in milk yield and related composition changes resulting from clinical mastitis in dairy cows. Preventive Veterinary Medicine 37 1–20CrossRefGoogle ScholarPubMed
Huijps, K, Lam, TJGM & Hogeveen, H 2008 Cost of mastitis: facts and perception. Journal of Dairy Research 75 113–120CrossRefGoogle ScholarPubMed
Jayarao, BM, Pillai, SR, Sawant, AA, Wolfgang, DR & Hegde, NV 2004 Guidelines for monitoring bulk tank milk somatic cell and bacterial counts. Journal of Dairy Science 87 3561–3573CrossRefGoogle ScholarPubMed
Jayarao, BM & Wolfgang, DR 2003 Bulk-tank milk analysis: a useful tool for improving milk quality and herd udder health. Veterinary Clinics of North America 19 75–92Google ScholarPubMed
Kitchen, BJ 1981 Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. Journal of Dairy Research 48 167–188CrossRefGoogle ScholarPubMed
Langoni, H, Silva, AV, Cabral, KG & Domingues, PF 1998 Etiologic aspects of bovine mastitis: aerobic bacterial flora. Brazilian Journal of Veterinary Medicine 20 204–209Google Scholar
Ma, Y, Barbano, DM, Galton, DM, Rudan, MA & Boor, KJ 2000 Effects of somatic cell count on quality and shelf-life of pasteurized fluid milk. Journal of Dairy Science 83 264–274CrossRefGoogle ScholarPubMed
Mazal, G, Vianna, PCB, Santos, MV & Gigante, ML 2007 Effect of somatic cell count on prato cheese composition. Journal of Dairy Science 90 630–636CrossRefGoogle ScholarPubMed
Merck 2008 The Merck Veterinary Manual. Mastitis in large animals: Introduction. Whitehouse Station: Merck & Co. Avaliable: <http://www.merckvetmanual.com/mvm/htm/bc/110900.htm> Acess: 10/15/2008+Acess:+10/15/2008>Google Scholar
Murphy, SC, Cranker, K, Senyk, GF, Barbano, DM, Seaman, AI & Galton, DM 1989 Influence of bovine mastitis on lipolysis and proteolysis in milk. Journal of Dairy Science 72 620–626CrossRefGoogle ScholarPubMed
Murray, PR, Baron, EJ, Pfaller, MA, Tenover, FC & Yolken, H 1999 Manual of Clinical Microbiology. 7th Edn, Washington DC, USA: American Society of MicrobiologyGoogle Scholar
Nader Filho, A, Schocken-Iturrino, RB & Rossi Júnior, OD 1983 Subclinical mastitis in dairy herds producing high quality milk type B. Brazilian Journal of Veterinarian and Animal Sciences 35 621–630Google Scholar
Nader Filho, A, Schocken-Iturrino, RB, Rossi Júnior, OD 1984 Subclinical mastitis in dairy herds producing milk type 3,2% fat. Brazilian Journal of Veterinarian and Animal Sciences, 36 549–558Google Scholar
National Mastitis Council 1987 Laboratory and Field Handbook on Bovine Mastitis. p. 288. Fort Atkinson WI, USA: Hoard & Sons Co.Google Scholar
Østerås, O 2005 Economic consequences of mastitis. Bulletin of the International Dairy Federation 394 25 ppGoogle Scholar
Park, YK, Koo, HC, Kim, SH, Hwang, SY, Jung, WK, Kim, JM, Shin, S, Kim, RT & Park, YH 2007 The analysis of milk components and pathogenic bacteria isolated from bovine raw milk in Korea. Journal of Dairy Science 90 5404–5414CrossRefGoogle ScholarPubMed
Phuektes, P, Browning, GF, Anderson, G & Mansell, PD 2003 Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in bulk milk samples. Journal of Dairy Research 70 149–155CrossRefGoogle ScholarPubMed
Piepers, S, Meulemeester, LD, Kruif, A, Opsomer, G, Barkema, HW & Vliegher, SD 2007 Prevalence and distribution of mastitis pathogens in subclinically infected dairy cows in Flanders, Belgium. Journal of Dairy Research 74 478–483CrossRefGoogle ScholarPubMed
Quinn, PJ, Markey, BK, Carter, ME, Donnelly, WJ & Leonard, FC 2005 Veterinary Microbiology and Microbial Disease. pp. 512. Porto Alegre, Brazil: ARTMEDGoogle Scholar
Randolph, HE & Erwin, RE 1974 Influence of mastitis on properties of milk. X. Fatty acid composition. Journal of Dairy Science 57 865–868CrossRefGoogle ScholarPubMed
Rogers, SA & Mitchell, GE 1994 The relationship between somatic cell count, composition and manufacturing properties of bulk milk. 6. Cheddar cheese and skim milk yoghurt. Australian Journal of Dairy Technology 49 70–74Google Scholar
Ruegg, PL 2003 Investigation of mastitis problems on farms. Veterinary Clinics of North America 19 47–74Google ScholarPubMed
Ruegg, PL & Tabone, TJ 2000 The relationship between antibiotic residue violations and somatic cell count in Wisconsin dairy herds. Journal of Dairy Science 83 2805–2809CrossRefGoogle ScholarPubMed
Rysanek, D & BabaK, V 2005 Bulk tank milk somatic cell count as an indicator of the hygiene status of primary milk production. Journal of Dairy Research 72 400–405CrossRefGoogle ScholarPubMed
Santos, MV, Ma, Y & Barbano, DM 2003 Effect of somatic cell count on proteolysis and lipolysis in pasteurized fluid milk during shelf-life storage. Journal of Dairy Science 86 2491–2503CrossRefGoogle ScholarPubMed
Schalm, OW & Noorlander, DO 1957 Experimental and observation leading to development of California Mastitis Test. Journal of American Veterinary Research 15 60–63Google Scholar
Schukken, YH, Grommers, FJ, van de Geer, D & Brand, A 1989 Incidence of clinical mastitis on farms with low somatic cell counts in bulk milk. Veterinary Record 125 60–63CrossRefGoogle ScholarPubMed
Somers, JM, O'Brien, B, Meaney, W & Kelly, AL 2003 Heterogeneity of proteolytic enzyme activities in milk samples of different somatic cell count. Journal of Dairy Research 70 45–50CrossRefGoogle ScholarPubMed
Sumner, J 1999 Hygiene management system on the farm. Bulletin of the International Dairy Federation 345 25Google Scholar
SPSS® 9.0 Statistics Software. SPSS Inc., Chicago IL, USAGoogle Scholar
Souto, LIM, Minagawa, CY, Telles, EO, Garbuglio, MA, Amaku, M, Dias, RA, Sakata, ST & Benites, NR 2008 Relationship between occurrence mastitis pathogens in dairy cattle herds and raw-milk indicators of hygienic-sanitary quality. Journal of Dairy Research 75 121–127CrossRefGoogle ScholarPubMed
Tenhagen, BA, Köster, G, Wallmann, J & Heuwieser, W 2006 Prevalence of mastitis pathogens and their resistance against antimicrobial agents in dairy cows in Brandenburg, Germany. Jorunal of Dairy Science 89 2542–2551CrossRefGoogle ScholarPubMed
US Food and Drugs Administration 2001 Center for Food Safety and Applied Nutrition. Bacteriological Analytical Manual (On line). Available: <http://www.cfsan.fda.gov/~ebam/bam-toc.html> Access: 08/20/2002+Access:+08/20/2002>Google Scholar
US Food and Drugs Administration 2003 Center for Food Safety and Applied Nutrition. Grade “A” Pasteurized Milk Ordinance (2003 Revision). Section 7 – Standards for Grade “A” milk and milk products. Available: <http://www.cfsan.fda.gov/~ear/pmo03-2.html> Access: 10/19/2008+Access:+10/19/2008>Google Scholar
US Food and Drugs Administration 2007 Center for Food Safety and Applied Nutrition. Grade “A” Pasteurized Milk Ordinance (2007 Revision). Section 7 – Standards for Grade “A” Milk and Milk Products. Available: <http://www.fda.gov/Food/FoodSafety/Product-SpecificInformation/MilkSafety/NationalConferenceonInterstateMilkShipmentsNCIMSModelDocuments/PasteurizedMilkOrdinance2007/default.htm> Access: 06/29/2009+Access:+06/29/2009>Google Scholar
Vianna, PCB, Mazal, G, Santos, MV, Bolini, HMA & Gigante, ML 2008 Microbial and sensory changes throughout the ripening of prato cheese made from milk with different levels of somatic cells. Journal of Dairy Science 91 1743–1750CrossRefGoogle ScholarPubMed
Zecconi, A & Hahn, G 1999 Staphylococcus aureus in raw milk and human health risk. Bulletin of the International Dairy Federation 345 15–18Google Scholar