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Contamination of pasteurized milk by Bacillus cereus in the filling machine

Published online by Cambridge University Press:  06 August 2001

ÅSA ENEROTH ,
BIRGITTA SVENSSON ,
GÖRAN MOLIN  and
ANDERS CHRISTIANSSON
ÅSA ENEROTH
Affiliation:
Laboratory of Food Hygiene, Department of Food Technology, Lund University, S-221 00 Lund, Sweden
BIRGITTA SVENSSON
Affiliation:
Swedish Dairy Association, Research and Development Dept., Scheelevögen 18, S-223 63 Lund, Sweden
GÖRAN MOLIN
Affiliation:
Laboratory of Food Hygiene, Department of Food Technology, Lund University, S-221 00 Lund, Sweden
ANDERS CHRISTIANSSON
Affiliation:
Swedish Dairy Association, Research and Development Dept., Scheelevögen 18, S-223 63 Lund, Sweden
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Abstract

The contamination of pasteurized milk by Bacillus cereus during the filling process was studied in two dairy plants. Samples of pasteurized milk were taken at four different sites along the production line. The samples were stored at 7 °C for 7 d, or at 10 °C for 5 d, before plate counting and random selection of B. cereus isolates. Isolates of B. cereus were typed by the polymerase chain reaction (PCR)-based method randomly amplified polymorphic DNA (RAPD). Samples taken at three different sites between the pasteurizer and the filling machine were all holding similar low concentrations of B. cereus, while an increase of the B. cereus count was seen in the consumer packages. More B. cereus of different RAPD types was growing in the consumer packages than in samples taken just before the filling machine. Several RAPD types found in the consumer packages were not detected in the samples taken just before the filling machine.

Keywords

Pasteurized milkBacillus cereusfilling machineRAPD method
Type
Original article
Information
Journal of Dairy Research , Volume 68 , Issue 2 , May 2001 , pp. 189 - 196
Copyright
Proprietors of Journal of Dairy Research 2001

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